Distribution of non-myelinating Schwann cells and their associations with leukocytes in mouse spleen revealed by immunofluorescence staining

  • Bin Ma Murdoch University, School of Veterinary and Life Sciences, Australia.
  • Changfu Yin Hebei Medical University, Clinical College, China.
  • Dailun Hu Hebei Medical University, Clinical College, China.
  • Mark Newman Murdoch University, School of Veterinary and Life Sciences, Australia.
  • Philip K. Nicholls Murdoch University, School of Veterinary and Life Sciences, Australia.
  • Zhanjun Wu Hebei Academy of Agriculture and Forestry Sciences, Institute of Cereal and Oil Crop, China.
  • Wayne K. Greene Murdoch University, School of Veterinary and Life Sciences, Australia.
  • Zhongli Shi | shizhongli@hebmu.edu.cn Hebei Medical University, Clinical College, China.

Abstract

The nervous system and the immune system communicate extensively with each other in order to maintain homeostasis and to regulate the immune response. The peripheral nervous system (PNS) communicates specifically with the immune system according to local interactions, including the “hardwiring” of sympathetic/parasympathetic (efferent) and sensory nerves (afferent) to lymphoid tissue and organs. To reveal this type of bidirectional neuroimmune interaction at the microscopic level, we used immunofluorescent staining of glial fibrillary acidic protein (GFAP) coupled with confocal microscopy/3D reconstruction to reveal the distribution of non-myelinating Schwann cells (NMSCs) and their interactions with immune cells inside mouse spleen. Our results demonstrate i) the presence of  an extensive network of  NMSC processes in all splenic compartments including the splenic nodules, periarteriolar lymphoid sheath (PALS), marginal zone,  trabecula, and red pulp; ii) the close association of  NMSC processes with blood vessels (including central artries and their branches, marginal sinuses, penicillar arterioles and splenic sinuses); iii) the close “synapse-like” interaction/association of NMSC processes with various subsets of dendritic cells (DCs; e.g., CD4+CD11c+ DCs, B220+CD11c+ DCs, and F4/80+ CD11c+ DCs), macrophages (F4/80+), and lymphocytes (B cells, CD4+ T helper cells). Our novel findings concerning the distribution of NMSCs and NMSC-leukocytes interactions inside mouse spleen should improve our understanding of the mechanisms through which the PNS affects cellular- and humoral-mediated immune responses in a variety of health conditions and infectious/non-infectious diseases.

Downloads

Download data is not yet available.
Published
2018-04-03
Section
Original Papers
Keywords:
Non-myelinating Schwann cells (NMSC), lymphocyte, dendritic cell (DC), spleen, immunofluorescence staining.
Statistics
Abstract views: 1176

PDF: 236
Supplementary: 34
HTML: 16
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Ma, B., Yin, C., Hu, D., Newman, M., Nicholls, P. K., Wu, Z., Greene, W. K., & Shi, Z. (2018). Distribution of non-myelinating Schwann cells and their associations with leukocytes in mouse spleen revealed by immunofluorescence staining. European Journal of Histochemistry, 62(2). https://doi.org/10.4081/ejh.2018.2890