Expression of acetylated tubulin in the postnatal developing mouse cochlea

  • Wenjing Liu Shanghai Jiaotong University Ear Institute, Department of Otorhinolaryngology-Head and Neck Surgery, China.
  • Chuanxi Wang Wannan Medical College, Department of Otorhinolaryngology-Head and Neck Surgery, China.
  • Hao Yu Anhui Province Key Laboratory of Biological Macro-Molecules Research, Wannan Medical College, China.
  • Shaofeng Liu Wannan Medical College, Department of Otorhinolaryngology-Head and Neck Surgery, China.
  • Jun Yang | yangjun@xinhuamed.com Shanghai Jiaotong University Ear Institute, Department of Otorhinolaryngology-Head and Neck Surgery, China.

Abstract

Acetylation tubulin is one of the major post-translational modifications of microtubules. Stable microtubules are well known to contain acetylated tubulin. Here, we examined the spatiotemporal expression of acetylated tubulin in the mouse cochlea during postnatal development. At postnatal day 1 (P1), acetylated tubulin was localized primarily to the auditory nerve inside the cochlea and their synaptic contacts with the inner and outer hair cells (IHCs and OHCs). In the organ of Corti, acetylated tubulin occurred first at the apex of pillar cells. At P5, acetylated tubulin first appeared in the phalangeal processes of Deiters’ cells. At P8, staining was maintained in the phalangeal processes of Deiters’ cells. At P10, labeling in Deiters’ cells extended from the apices of OHCs to the basilar membrane. Labeling was expressed throughout the cytoplasm of pillar cells. At P12, acetylated tubulin displayed prominent and homogeneous labeling along the full length of the pillar cells. Linear labeling was present mainly in the Deiters’ cell bodies underlying OHCs. Between P14 and P17, acetylated tubulin was strongly expressed in inner and outer pillar cells and Deiters’ cells in a similar pattern as observed in the adult, and labeling in these cells were arranged in bundles. In addition, acetylated tubulin was intensely expressed in stria vascularis, root cell bodies, and a small number of fibrocytes of the spiral ligament until the adult. In the adult mouse cochlea, immunostaining continued to predominate in Deiters’ cells and pillar cells. Immunolabeling formed cups securing OHCs basal portions, and continued presence of acetylated tubulin-labeled nerve terminals below IHCs was shown. Our results presented here underscored the essential role played by acetylated tubulin in postnatal cochlear development, auditory neurotransmission and cochlear mechanics.

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Published
2018-08-08
Section
Original Papers
Supporting Agencies
National Natural Science Foundation of China (No.81170919, No. 81470689);, Shanghai Municipal Science and Technology Commission (No. 14DJ1400201, No. 14DZ2260300).
Keywords:
Acetylated tubulin, immunofluorescence, expression, mouse, cochlea, development.
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How to Cite
Liu, W., Wang, C., Yu, H., Liu, S., & Yang, J. (2018). Expression of acetylated tubulin in the postnatal developing mouse cochlea. European Journal of Histochemistry, 62(3). https://doi.org/10.4081/ejh.2018.2942