Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results

  • Cecilia Dall'Aglio Department of Veterinary Medicine, University of Perugia, Italy.
  • Paola Scocco School of Biosciences and Veterinary Medicine, University of Camerino, Italy.
  • Margherita Maranesi | margherita.maranesi@unipg.it Department of Veterinary Medicine, University of Perugia, Italy.
  • Linda Petrucci Department of Veterinary Medicine, University of Perugia, Italy.
  • Gabriele Acuti Department of Veterinary Medicine, University of Perugia, Italy.
  • Elena De Felice School of Biosciences and Veterinary Medicine, University of Camerino, Italy.
  • Francesca Mercati

Abstract

Resistin is a polypeptide hormone of the adipokine-family, primarily, but not exclusively, produced by the adipose tissue. Recent studies suggested that resistin may affect the male and female reproductive activity. The study aim was to immunohistochemically evaluate the presence and distribution of resistin in the ovine uterus. Uterine samples were collected from two groups of ewes at the end of an experimental trial during which the animals of the first group (CTRL) were fed only by grazing while those of the second one (EXP) were supplemented with barley and corn. Using a monoclonal antibody against resistin, tested by Western Blot, the immunopositive reaction was identified in the cytoplasm of epithelial lining cells and uterine glands. The endogenous production of resistin seemed to be affected by different diet, as evidenced by staining differences between the CTRL and EXP groups. Our findings support the existence of a peripheral resistin system in the sheep uterus. It is possible that this system is involved in the functionality of the uterus, which is also affected by the animal’s nutritional status. 

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Published
2019-05-03
Section
Brief Reports
Keywords:
Sheep, resistin, immunohistochemistry, uterus, diet
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How to Cite
Dall’Aglio, C., Scocco, P., Maranesi, M., Petrucci, L., Acuti, G., De Felice, E., & Mercati, F. (2019). Immunohistochemical identification of resistin in the uterus of ewes subjected to different diets: Preliminary results. European Journal of Histochemistry, 63(2). https://doi.org/10.4081/ejh.2019.3020

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