https://doi.org/10.4081/ejh.2019.3026
Embedding cell monolayers to investigate nanoparticle-plasmalemma interactions at transmission electron microscopy
Accepted: 26 March 2019
HTML: 20
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Transmission electron microscopy is the technique of choice to visualize the spatial relationships between nanoconstructs and cells and especially to monitor the uptake process of nanomaterials. It is therefore crucial that the cell surface be preserved in its integrity, to obtain reliable ultrastructural evidence: the plasmalemma represents the biological barrier the nanomaterials have to cross, and the mode of membrane-nanoconstruct interaction is responsible for the intracellular fate of the nanomaterials. In this paper, we describe a simple and inexpensive method to process cell monolayers for ultrastructural morphology and immunocytochemistry, ensuring consistent preservation of the cell surface and of the occurring interactions with nanoparticles of different chemical composition.
How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Zhiyang Wang, Jing Li, Ziwei Liu, Ling Yue, Nrf2 as a novel diagnostic biomarker for papillary thyroid carcinoma , European Journal of Histochemistry: Vol. 67 No. 2 (2023)
- R. M. Ruggeri, E. Vitarelli, G. Barresi, F. Trimarchi, S. Benvenga, M. Trovato, The tyrosine kinase receptor c-met, its cognate ligand HGF and the tyrosine kinase receptor trasducers STAT3, PI3K and RHO in thyroid nodules associated with Hashimoto's thyroiditis: an immunohistochemical characterization , European Journal of Histochemistry: Vol. 54 No. 2 (2010)
- Andrea Conz, Clara Alice Musi, Luca Russo, Tiziana Borsello, Luca Colnaghi, Super-resolution study of PIAS SUMO E3-ligases in hippocampal and cortical neurons , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
- Laura Calderan, Flavia Carton, Ilaria Andreana, Valeria Bincoletto, Silvia Arpicco, Barbara Stella, Manuela Malatesta, An ex vivo experimental system to track fluorescent nanoparticles inside skeletal muscle , European Journal of Histochemistry: Vol. 67 No. 1 (2023)
- Meiying Zhang, Min Zhou, Xia Cai, Yan Zhou, Xueling Jiang, Yan Luo, Yue Hu, Rong Qiu, Yanrong Wu, Yuejin Zhang, Yan Xiong, VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-κB/ICAM-1 signaling pathway , European Journal of Histochemistry: Vol. 66 No. 4 (2022)
- G. Caocci, M. Greco, D. Fanni, G. Senes, R. Littera, S. Lai, P. Risso, C. Carcassi, G. Faa, G. La Nasa, HLA-G expression and role in advanced-stage classical Hodgkin lymphoma , European Journal of Histochemistry: Vol. 60 No. 2 (2016)
- W. Chen, J. Liang, L. Huang, J. Cai, Y. Lei, J. Lai, L. Liang, K. Zhang, Characterizing the activation of the Wnt signaling pathway in hilar cholangiocarcinoma using a tissue microarray approach , European Journal of Histochemistry: Vol. 60 No. 1 (2016)
- T. Petr, V. Å mÃd, J. Å mÃdová, H. Hůlková, M. Jirkovská, M. Elleder, L. Muchová, L. Vitek, F. Å mÃd, Histochemical detection of GM1 ganglioside using cholera toxin-B subunit. Evaluation of critical factors optimal for in situ detection with special emphasis to acetone pre-extraction , European Journal of Histochemistry: Vol. 54 No. 2 (2010)
- Liqin Xi, Chen Wang, Pengyu Chen, Qi Yang, Ruiqi Hu, Haolin Zhang, Qiang Weng, Meiyu Xu, Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii) , European Journal of Histochemistry: Vol. 61 No. 4 (2017)
- L. Wang, J. Wang, X. Zhou, J. Li, Y. Shi, Z. Han, X. Wang, S. Li, Z. Yang, R. Wang, D. Fan, Y. Han, CM2 antigen, a potential novel molecule participating in glucuronide transport on rat hepatocyte canalicular membrane , European Journal of Histochemistry: Vol. 56 No. 3 (2012)
<< < 31 32 33 34 35 36 37 38 39 40 > >>
You may also start an advanced similarity search for this article.
