Ferritin expression in the periodontal tissues of primates

  • Wenxue Huang | hwx_1027@163.com Peking University School and Hospital of Stomatology, China.
  • Wei Li Department of Periodontology, The Affiliated Hospital of Qingdao University; Key Laboratory of Oral Clinical Medicine, College of Stomatology, Qingdao University, China.
  • Juan Liu Department of Periodontology, Peking University School and Hospital of Stomatology, China.
  • Jianxia Hou Department of Periodontology, Peking University School and Hospital of Stomatology, China.
  • Huanxin Meng Department of Periodontology, Peking University School and Hospital of Stomatology, China.

Abstract

Ferritin, an iron-binding protein, is composed of two subunits, a heavy chain and a light chain. It regulates many biological functions, such as proliferation, angiogenesis, and immunosuppression. The objective of this study was to determine the expression and distribution of ferritin in the periodontal tissuesof primates.First, we assessed the expression of ferritin in primary cultured cells isolated from human periodontal tissues using the polymerase chain reaction and immunofluorescent staining. Second, we investigated the expression and distribution of ferritin in the periodontal tissues of Macaca fascicularis, human gingival tissues, and human gingival carcinoma tissues using immunohistochemistry.Both protein and mRNA of ferritin were constitutively present in human primary cultured cells, including those from the dental apical papilla, periodontal ligament, dental pulp, and gingival epithelium, as well as gingival fibroblasts. In M. fascicularistissues, the immunohistochemical staining was particularly strong in blood vessel and mineralizing areas of the dental pulp and periodontal ligament. Ferritin heavy chain exhibited specific immunopositivity in in the stratum basale of the epithelium in human gingival tissue and strong immunostaining was found in peripheral regions of gingival carcinoma sites. Ferritin is constitutivelypresent andwidelydistributed in the periodontal tissues of primates. Ferritin may play roles in epithelial proliferation, vascular angiogenesis, and mineralization in these tissues.

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Published
2019-09-03
Section
Original Papers
Supporting Agencies
National Natural Science Foundation of China (No. 81470738 to JXH)
Keywords:
Ferritin, ferritin heavy chain, Macaca fascicularis, periodontal ligament, dental pulp, gingiva, carcinoma of gingiva
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How to Cite
Huang, W., Li, W., Liu, J., Hou, J., & Meng, H. (2019). Ferritin expression in the periodontal tissues of primates. European Journal of Histochemistry, 63(3). https://doi.org/10.4081/ejh.2019.3046