https://doi.org/10.4081/ejh.2019.3049
Molecular characterization of nephron progenitors and their early epithelial derivative structures in the nephrogenic zone of the canine fetal kidney
Accepted: 10 September 2019
HTML: 44
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Nephron progenitors (NPs) and nephrogenesis have been extensively studied in mice and humans and have provided insights into the mechanisms of renal development, disease and possibility of NP-based therapies. However, molecular features of NPs and their derivatives in the canine fetal kidney (CFK) remain unknown. This study was focused to characterize the expression of potential markers of canine NPs and their derivatives by immuno-fluorescence and western blot analysis. Transcription factors (TFs) SIX1 and SIX2, well-characterized human NP markers, were expressed in NPs surrounding the ureteric bud in the CFK. Canine NPs also expressed ITGA8 and NCAM1, surface markers previously used to isolate NPs from the mouse and human fetal kidneys. TF, PAX2 was detected in the ureteric bud, NPs and their derivative structures such as renal vesicle and S-shaped body. This study highlights the similarities in dog, mouse and human renal development and characterizes markers to identify canine NPs and their derivatives. These results will facilitate the isolation of canine NPs and their functional characterization to develop NP-based therapies for canine renal diseases.
Supporting Agencies
This research was supported by funds provided by the Tuskegee University College of Veterinary Medicine (PP), Research in principal investigator's laboratory is supported by funding from the 1SC2GM130475-01(PP) grant awarded by the National Institute of General Medical Sciences, AIA was supported by NIH/SRE 5T35OD010432How to Cite
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Roberta Sferra, Simona Pompili, Luca Ventura, Caroline Dubuquoy, Silvia Speca, Eugenio Gaudio, Giovanni Latella, Antonella Vetuschi, Interaction between sphingosine kinase/sphingosine 1 phosphate and transforming growth factor-β/Smads pathways in experimental intestinal fibrosis. An in vivo immunohistochemical study , European Journal of Histochemistry: Vol. 62 No. 3 (2018)
- E. Tarantola, V. Bertone, G. Milanesi, C. Gruppi, A. Ferrigno, M. Vairetti, S. Barni, I. Freitas, Dipeptidylpeptidase-IV activity and expression reveal decreased damage to the intrahepatic biliary tree in fatty livers submitted to subnormothermic machine-perfusion respect to conventional cold storage , European Journal of Histochemistry: Vol. 58 No. 3 (2014)
- Valeriia Gusel'nikova, Olga Antimonova, Elena Fedorova, Mikhail Shavlovsky, Aleksandr Krutikov, Ekaterina Mikhailova, Aleksandra Gudkova, Vyacheslav Mikhailov, Dmitry Korzhevskii, Fluorescent characterization of amyloid deposits in the kidneys of mdx mice , European Journal of Histochemistry: Vol. 62 No. 2 (2018)
- H. Zhang, Y. Wang, J. Zhang, L. Wang, Q. Li, X. Sheng, Y. Han, Z. Yuan, Q. Weng, Testicular expression of NGF, TrkA and p75 during seasonal spermatogenesis of the wild ground squirrel (Citellus dauricus Brandt) , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- C. Bai, D. Wang, C. Li, D. Jin, C. Li, W. Guan, Y. Ma, Establishment and biological characteristics of a Jingning chicken embryonic fibroblast bank , European Journal of Histochemistry: Vol. 55 No. 1 (2011)
- J. Melrose, The knee joint loose body as a source of viable autologous human chondrocytes , European Journal of Histochemistry: Vol. 60 No. 2 (2016)
- G Stabellini, M Calvitti, T Baroni, L Marinucci, C Calastrini, P Carinci, E Becchetti, Glycosidases during chick embryo lung development and their colocalization with proteoglycans and growth factors , European Journal of Histochemistry: Vol. 46 No. 1 (2002)
- S. Battistelli, B. Citterio, B. Baldelli, C. Parlani, M. Malatesta, Histochemical and morphometrical study of mouse intestine epithelium after a long term diet containing genetically modified soybean , European Journal of Histochemistry: Vol. 54 No. 3 (2010)
- Roberto Imberti, Alessandro Amatu, Drug delivery to the central nervous system , European Journal of Histochemistry: Vol. 54 No. 3 (2010)
- Enrica Cappellozza, Federico Boschi, Maddalena Sguizzato, Elisabetta Esposito, Rita Cortesi, Manuela Malatesta, Laura Calderan, A spectrofluorometric analysis to evaluate transcutaneous biodistribution of fluorescent nanoparticulate gel formulations , European Journal of Histochemistry: Vol. 66 No. 1 (2022)
<< < 25 26 27 28 29 30 31 32 33 34 > >>
You may also start an advanced similarity search for this article.
