Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii)

  • Yue Zhang College of Biological Science and Technology, Beijing Forestry University, Beijing , China.
  • Ao Zhang College of Biological Science and Technology, Beijing Forestry University, Beijing, China.
  • Yue Zhao College of Biological Science and Technology, Beijing Forestry University, Beijing, China.
  • Xiaohang Feng College of Biological Science and Technology, Beijing Forestry University, Beijing, China.
  • Yuyao Sheng College of Biological Science and Technology, Beijing Forestry University, Beijing, China.
  • Haolin Zhang College of Biological Science and Technology, Beijing Forestry University, Beijing, China.
  • Qiang Weng College of Biological Science and Technology, Beijing Forestry University, Beijing, China.
  • Meiyu Xu | xumeiyu@bjfu.edu.cn College of Biological Science and Technology, Beijing Forestry University, Beijing, China.

Abstract

One special physiological phenomenon of the Chinese brown frog (Rana dybowskii) is that its oviduct goes through expansion prior to pre-hibernation instead of expanding during the breeding period. Our previous study discovered that some cytokines such as interleukin-1 beta (IL-1β) and its receptor type I (IL1R1) proteins were expressed in the oviduct of Rana dybowskii. In this study, we continued to investigate the localizations and expression levels of cytokines including toll-like receptor 4 (TLR4) and its related factors, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase (IRAK) and nuclear factor kappa B (NF-κΒ) in the oviduct of Rana dybowskii during the breeding period and pre-hibernation. Morphological results showed that there were significant differences in oviductal weight and pipe diameter with significantly higher values in pre-hibernation than those of the breeding period. Strong immunostaining of TLR4, MyD88, IRAK4 and NF-κΒ were observed in the pre-hibernation. These proteins levels had no significant difference between these two periods except MyD88 which was significantly higher in the pre-hibernation. The mRNA expression levels of MyD88 and NF-κΒ were obviously higher in pre-hibernation than those of the breeding period. These findings suggested that TLR4/MyD88 signal pathway might participate in the oviductal functions of Rana dybowskii during the breeding period and pre-hibernation.

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Histological structure of Rana dybowskii oviducts in the breeding period
Published
2019-09-25
Section
Original Papers
Supporting Agencies
Beijing Natural Science Foundation (8182039), National Natural Science Foundation of China (31872320, 21806010), Young Scientist Start-up funding of Beijing Forestry University (BLX201714)
Keywords:
Rana dybowskii, oviduct, MyD88, TLR4, IRAK4, NF-κΒ
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How to Cite
Zhang, Y., Zhang, A., Zhao, Y., Feng, X., Sheng, Y., Zhang, H., Weng, Q., & Xu, M. (2019). Expressions of TLR4, MyD88, IRAK4 and NF-κΒ in the oviduct of Chinese brown frog (Rana dybowskii). European Journal of Histochemistry, 63(3). https://doi.org/10.4081/ejh.2019.3050