Dark-field microscopy enhance visibility of CD31 endothelial staining

Abstract

A simple dark field microscopy technique was used for visualization of blood vessels in normal human renal tissues and carcinoma. Phase contrast condenser ring apt for high power objectives was combined with a 10x objective in order to create a dark field illumination of the specimens examined. The endothelial lining of the vessels had been stained by using CD31 monoclonal antibodies combined with conventional peroxidase immunohistochemistry. The final DAB addition used for this technique induced an intense light scatter in the dark field microscope. This scattered light originating from the endothelial lining made the walls of the bright vessels easily detectable from the dark background.

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Published
2020-07-01
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Section
Technical Notes
Keywords:
dark field, DAB, CD31, kidney
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How to Cite
Jennische, E., Lange, S., & Hultborn, R. (2020). Dark-field microscopy enhance visibility of CD31 endothelial staining . European Journal of Histochemistry, 64(3). https://doi.org/10.4081/ejh.2020.3133