https://doi.org/10.4081/ejh.2020.3135

RETRACTED: Neural stem cell conditioned medium alleviates Aβ25-35 damage to SH-SY5Y cells through the PCMT1/MST1 pathway

Vol. 64 No. s2 (2020): Special Collection on Stem Cells and Regenerative Medicine
Submitted: 28 March 2020
Accepted: 29 May 2020
Published: 19 June 2020
Neural stem cell conditioned medium, apoptosis, amyloid β25-35, SH-SY5Y cells, protein L-Isoaspartate (D-Aspartate) O-Methyltransferase (PCMT1)
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Authors

Guoyong Jia
https://orcid.org/0000-0002-7418-0628
Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Hongna Yang
Department of Critical-care Medicine, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Zengyan Diao
https://orcid.org/0000-0002-1848-5953
Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Ying Liu
Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Congcong Sun
suncongcong@sdu.edu.cn
https://orcid.org/0000-0001-5343-4425
Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.

Alzheimer’s disease (AD) is a progressive, neurodegenerative disease. Accumulating evidence suggests that protein isoaspartate methyltransferase 1 (PCMT1) is highly expressed in brain tissue (substantia nigra, blue plaque, paraventricular nucleus). In this study, we investigated the effect of neural stem cell conditioned medium alleviates Aβ25-35 damage to SH-SY5Y cells by PCMT1/MST1 pathway. Results demonstrated that Aβ25-35 significantly decreased the cell viability in time and dose dependent manner. However, Neural stem cell-complete medium (NSC-CPM) or NSC-CDM had inhibitory effect on toxicity when fibrillation of Aβ25-35 occurred in their presence and NSC-CDM had a better inhibitor result. An increase of the PCMT1 expression levels was found in Aβ25-35 + NSC-CDM group. sh-PCMT1 significantly reduced the PCMT1, the cell viability and inhibited the protective effect; induced apoptosis and increased the expression of p-MST1. Overexpression of PCMT1 group reversed the effect of Aβ25-35 inhibited the cell viability and Aβ25-35 induced the apoptosis. In conclusion, NSC-CDM corrects the damage of Aβ25-35 to cells by increasing PCMT1, reducing MST phosphorylation.

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How to Cite

Jia, G., Yang, H., Diao, Z., Liu, Y., & Sun, C. (2020). <b>RETRACTED</b>: Neural stem cell conditioned medium alleviates Aβ<sub>25-35</sub> damage to SH-SY5Y cells through the PCMT1/MST1 pathway. European Journal of Histochemistry, 64(s2). https://doi.org/10.4081/ejh.2020.3135

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