An emerging potential therapeutic target for osteoporosis: LncRNA H19/miR-29a-3p axis

https://doi.org/10.4081/ejh.2020.3155

Authors

  • Ziqi Li Department of Joint Disease, Traumatology and Orthopedics institute of Guangzhou University of Chinese Medicine, the third affiliated hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Zhinan Hong Department of Joint Disease, Traumatology and Orthopedics institute of Guangzhou University of Chinese Medicine, the third affiliated hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Yuesheng Zheng Department of Orthopedics, Jinshazhou hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Yongwei Dong Department of Orthopedics, Jinshazhou hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Wei He Department of Orthopedics, Jinshazhou hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Yingjia Yuan Department of Joint Disease, Traumatology and Orthopedics institute of Guangzhou University of Chinese Medicine, the third affiliated hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
  • Junbiao Guo | liziqidoc@gmail.com Guangdong Provincial Hospital of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. https://orcid.org/0000-0003-1760-6298

Abstract

Osteoporosis (OP) is a complex systemic disease characterized by a loss of bone density, leading to bone fragility and an increase risk of fractures of the hip, spine and wrist. The clinical therapeutic effect is still far from satisfactory. Thus, further studies are urgently needed to explore the pathogenesis of OP. In this study, our aim is to explore the underlying molecular mechanism of lncRNA H19/miR-29a-3p axis for regulating of inflammation, proliferation and apoptosis in OP. The expression of lncRNA H19 was significantly upregulated in OP samples compared with the health control. Subsequently, we found that miR-29a-3p is the target of lncRNA H19 in OP. Furthermore, the knockdown of lncRNA H19 was validated to promote the expression of pro-inflammatory mediators, repress cell proliferation and inhibit cell apoptosis in vitro. Moreover, the modulating effects of lncRNA-H19 on the expressions of pro-inflammatory mediators, cell proliferation and apoptosis in vitro were diminished after co-transfecting with miR-29a-3p inhibitor and siRNA-H19. Thus, we concluded that lncRNA H19/miR-29a-3p axis was involved in the development of OP. This study might provide a better understanding of OP development and a potential therapeutic target for OP intervention.

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Published
2020-11-06
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Corresponding Author
Ethical approval was obtained for all experimental procedures by the Ethics Committee of the Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China
Keywords:
Target therapy, lncRNA H19, miR-29a-3p, osteoporosis
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How to Cite
Li, Z., Hong, Z., Zheng, Y., Dong, Y., He, W., Yuan, Y., & Guo, J. (2020). An emerging potential therapeutic target for osteoporosis: LncRNA H19/miR-29a-3p axis. European Journal of Histochemistry, 64(4). https://doi.org/10.4081/ejh.2020.3155