Effects mediated by the α7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells

Submitted: 13 July 2020
Accepted: 25 September 2020
Published: 30 October 2020
Abstract Views: 712
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Adipose-derived stem cells (ASCs) are an attractive source for regenerative medicine as they can be easily isolated, rapidly expandable in culture and show excellent in vitro differentiation potential. Acetylcholine (ACh), one of the main neurotransmitters in central and peripheral nervous systems, plays key roles in the control of several physiological processes also in non-neural tissues. As demonstrated in our previous studies, ACh can contribute to the rat ASCs physiology, negatively modulating ASCs proliferation and migration via M2 muscarinic receptor (mAChR) activation. In the present work we show that rat ASCs also express α7 nicotinic receptors (nAChRs). In particular, we have investigated the effects mediated by the selective activation of α7 nAChRs, which causes a reduction of ASC proliferation without affecting cell survival and morphology, and significantly promotes cell migration via upregulation of the CXCR4 expression. Interestingly, the activation of the α7 nAChR also upregulates the expression of M2 mAChR protein, indicating a cooperation between muscarinic and nicotinic receptors in the inhibition of ASC proliferation.  

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Roberta Piovesana, Department of Biology and Biotechnologies “Charles Darwin”, Sapienza University of Rome

Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester, UK

Adam J. Reid, Department of Plastic Surgery and Burns, Wythenshawe Hospital, University of Manchester

Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester

How to Cite

Pernarella, M., Piovesana, R., Matera, C., Faroni, A., Fiore, M., Dini, L., … Tata, A. M. (2020). Effects mediated by the α7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells. European Journal of Histochemistry, 64(s2). https://doi.org/10.4081/ejh.2020.3159

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