https://doi.org/10.4081/ejh.2020.3159

Effects mediated by the α7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells

Vol. 64 No. s2 (2020): Special Collection on Stem Cells and Regenerative Medicine
Submitted: 13 July 2020
Accepted: 25 September 2020
Published: 30 October 2020
Acetylcholine, adipose-derived stem cells, alpha7 nicotinic receptor, proliferation, migration
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Authors

Marta Pernarella
Department of Biology and Biotechnologies “Charles Darwin”, Sapienza University of Rome, Italy.
Roberta Piovesana
https://orcid.org/0000-0003-2682-4473
Department of Biology and Biotechnologies “Charles Darwin”, Sapienza University of Rome, Italy.
Carlo Matera
https://orcid.org/0000-0001-6939-3859
Department of Pharmaceutical Sciences, Medicinal Chemistry Section “Pietro Pratesi”, University of Milan, Italy.
Alessandro Faroni
https://orcid.org/0000-0003-4435-6423
Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester, United Kingdom.
Mario Fiore
Institute of Molecular Biology and Pathology-CNR, Rome, Italy.
Luciana Dini
https://orcid.org/0000-0002-2633-9040
Department of Biology and Biotechnologies “Charles Darwin”, Sapienza University of Rome, Italy.
Adam J. Reid
https://orcid.org/0000-0003-1752-3302
Department of Plastic Surgery and Burns, Wythenshawe Hospital, University of Manchester, United Kingdom.
Clelia Dallanoce
Department of Pharmaceutical Sciences, Medicinal Chemistry Section “Pietro Pratesi”, University of Milan, Italy.
Ada Maria Tata
adamaria.tata@uniroma1.it
https://orcid.org/0000-0003-4868-5435
Department of Biology and Biotechnologies “Charles Darwin”; Research Centre of Neurobiology “Daniel Bovet”, Sapienza University of Rome, Italy.

Adipose-derived stem cells (ASCs) are an attractive source for regenerative medicine as they can be easily isolated, rapidly expandable in culture and show excellent in vitro differentiation potential. Acetylcholine (ACh), one of the main neurotransmitters in central and peripheral nervous systems, plays key roles in the control of several physiological processes also in non-neural tissues. As demonstrated in our previous studies, ACh can contribute to the rat ASCs physiology, negatively modulating ASCs proliferation and migration via M2 muscarinic receptor (mAChR) activation. In the present work we show that rat ASCs also express α7 nicotinic receptors (nAChRs). In particular, we have investigated the effects mediated by the selective activation of α7 nAChRs, which causes a reduction of ASC proliferation without affecting cell survival and morphology, and significantly promotes cell migration via upregulation of the CXCR4 expression. Interestingly, the activation of the α7 nAChR also upregulates the expression of M2 mAChR protein, indicating a cooperation between muscarinic and nicotinic receptors in the inhibition of ASC proliferation.  

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Roberta Piovesana, Department of Biology and Biotechnologies “Charles Darwin”, Sapienza University of Rome

Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester, UK

Adam J. Reid, Department of Plastic Surgery and Burns, Wythenshawe Hospital, University of Manchester

Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, University of Manchester

How to Cite

Pernarella, M., Piovesana, R., Matera, C., Faroni, A., Fiore, M., Dini, L., … Tata, A. M. (2020). Effects mediated by the α7 nicotinic acetylcholine receptor on cell proliferation and migration in rat adipose-derived stem cells. European Journal of Histochemistry, 64(s2). https://doi.org/10.4081/ejh.2020.3159

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