Swertiamarin suppresses proliferation, migration, and invasion of hepatocellular carcinoma cells via negative regulation of FRAT1

https://doi.org/10.4081/ejh.2020.3169

Authors

  • Shufeng Xiao Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming; Department of General Surgery, Puer People’s Hospital, Puer, China.
  • Haoren Tang Department of Gastroenterological Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Yao Bai School of Medicine, Yunnan University, Kunming, China.
  • Renchao Zou Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming 2Department of General Surgery, Puer People’s Hospital, Puer, China.
  • Zongfang Ren Department of Critical Care Medicine, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Xuesong Wu Department of Gastroenterological Surgery, the Second Affiliated Hospital of Kunming Medical University, China.
  • Zhitian Shi Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Song Lan Department of Pathology, Puer People’s Hospital, Puer, China.
  • Wei Liu Department of Hepatobiliary Surgery, The People’s Hospital of Chuxiong Yi Autonomous Prefecture, the Fourth Affiliated Hospital of Dali University, Chuxiong, China.
  • Tiangen Wu Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Cheng Zhang Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
  • Lin Wang | linwang705@gmail.com Department of Hepatobiliary Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China. https://orcid.org/0000-0003-4554-9198

Abstract

Studies have shown that swertiamarin (STM) has multiple biological activities, but its anti-tumour effects and molecular mechanisms are still unclear. The present research aimed to validate the STM’s impacts on the proliferation, migration, and invasion of hepatocellular carcinoma (HCC) cells, and to study its potential mechanism. Two HCC cell lines were treated with STM. Tumour growth was observed by the mouse tumour xenografts model. HCC cell lines stably expressing T-cell lymphomas 1 (FRAT1) were generated by lentivirusmediated overexpression. Cell viability, proliferation, migration, and invasion were observed using Cell Counting Kit-8 (CCK8), the xCELLigence Real-Time Cell Analyzer system (RTCA), and transwell analysis, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting were used to observe the expression of FRAT1 and proteins related to the Wnt/β-catenin signalling pathway. Tumour growth was inhibited by STM in vivo. STM suppressed the proliferation, migration, and invasion of HCC cells. STM negatively regulated FRAT1 expression, whereas overexpressed FRAT1 blocked the anti-tumour function of STM. The results revealed that STM suppressed the FRAT1/Wnt/β-catenin signalling pathway. The findings of this study provide new insights into investigation of therapeutic strategies against HCC.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Downloads

Published
2020-10-19
Info
Issue
Section
Articles
Corresponding Author
This study was approved by the Administration Committee of Experiment Animals of The Second Affiliated Hospital of Kunming Medical University
Supporting Agencies
Yunnan Hepatobiliary and Pancreatic Disease Clinical Center Fund, National Natural Science Foundation of China
Keywords:
Swertiamarin, FRAT1, HCC, proliferation, migration, invasion
Statistics
  • Abstract views: 251

  • PDF: 150
  • HTML: 0
How to Cite
Xiao, S., Tang, H., Bai, Y., Zou, R., Ren, Z., Wu, X., Shi, Z., Lan, S., Liu, W., Wu, T., Zhang, C., & Wang, L. (2020). Swertiamarin suppresses proliferation, migration, and invasion of hepatocellular carcinoma cells <em>via</em> negative regulation of FRAT1. European Journal of Histochemistry, 64(4). https://doi.org/10.4081/ejh.2020.3169

Most read articles by the same author(s)