Mon, 29 Mar 2021 in European Journal of Histochemistry
The clinical significance of UBE2C gene in progression of renal cell carcinoma
Abstract
Renal cell carcinoma (RCC), with high morbidity and mortality, is one of the top ten serious cancers. Due to limited therapies and little knowledge about the mechanism underlying RCC, overall survival of RCC patients is poor. UBE2C is a member of ubiquitin modification system and promotes carcinogenesis in cancer, but its role in RCC is unknown. Based on the TCGA (The Cancer Genome Atlas) data, UBE2C was over-expressed in a total of 525 RCC tissues and displayed higher expression in advanced tissues (stage IV vs stage I, p<0.05). RT-qPCR and IHC analysis confirmed over-expression of UBE2C in 90 of clinical RCC tissues. Further, UBE2C was associated with clinical factors including TNM stage, gender, and pathological stage. And higher UBE2C expression predicted shorter overall survival and progression-free survival. Both univariate and multivariate COX analysis suggested UBE2C as a critical gene in RCC. Then GO and KEGG analysis showed that cell cycle and DNA replication pathways were two top signaling pathways affected by UBE2C. In vitro assay showed that knockdown of UBE2C in 786-O cells inhibited proliferation and migration significantly. Therefore, this study proves that UBE2C is an important gene in RCC and is essential to proliferation and migration of RCC.
Main Text
Introduction
Renal cell carcinoma (RCC) is a malignant disease and originates from renal parenchyma urinary tubule epithelial system.1 Among them, clear cell carcinomas the major type and accounts for about 70% of all RCC patients.1-3 The main therapy for RCC is resection assisted with chemo-radiotherapy. But RCC is not so sensitive to radiotherapy or chemotherapytherapy.3 In recent years, target therapy is used to treat RCC and about 20-40% of RCC patients could benefit from target therapy.4,5 However, long-term survival is not easily acquired.6 According to the latest report released by AACR in 2019, the five-year survival rate for patients with distant metastasis is only 12%.7 And about 16% of patients are diagnosed with distant metastasis. In addition, a total of 73,820 persons will be subjected to kidney cancer and about 14,000 will die.7 This is partially due to the ignorance of mechanism underlying RCC.
Ubiquitin-conjugating enzyme E2C (UBE2C) is a member of the ubiquitin coupling enzyme E2 family and interacts with specific E3 enzymes, which leads to degradation of substrate proteins.8 The substrate proteins of UBE2C are cell cycle-related proteins involved in regulation of cell mitosis. Recent studies indicated that UBE2C played an oncogenic role in several types of cancer. For example, the abnormal expression of UBE2C caused chromosomal instability and participated in development and progression in gastric cancer.9 UBE2C is expressed abundantly in breast cancer,10 colon cancer,11 ovarian cancer,12 and liver cancer.11 And UBE2C was shown to be closely correlated with tumor stage, which suggests that UBE2C might be a new tumor marker and a promising therapeutic target in these tumors.10-13 However, the expression of UBE2C in RCC and its clinical value remain unclear. The cancer genome atlas (TCGA) database is a public database consisted of cancer genomics from over 20,000 cases of primary cancer and matched normal samples spanning 33 cancer types.14 The database contributes greatly to the research on elucidation of molecular mechanism underlying tumorigenesis. 15
In order to evaluate the clinical significance of UBE2C in RCC, we extracted the clinical information of RCC patients and UBE2C expression profile in RCC tissues from TCGA database. Then the relationship of UBE2C with pathological factors and prognoses of RCC patients was analyzed. And the potential process as well as signaling pathways were explored.
Materials and Methods
Bioinformatics analysis and TCGA mining
In order to analyze the differential expression levels of UBE2C in RCC and normal renal tissues, TCGA database was extracted. The mRNA expression level of UBE2C from a total of 525 cases of RCC and 72 cases of normal renal tissues was downloaded from the TCGA database (https://cancergenome.nih.gov/). Then, RStudio (Version 1.1.442), a free and open-source data analysis software, was used to analyze the differential expression of UBE2C and to deduce the overall survival (OS) and disease-free survival (DFS) curves. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used in conjunction with DAVID 6.8 and Enrichment Map plug-in Cytoscape to visualize the significant pathways and differential expression genes (DEGs) in RCC.
RCC tissues and pathological information of patients
A total of 90 RCC tissues and matched normal tissues were retrospectively collected to perform RT-qPCR analysis. All the patients had received radical operation in our hospital from July 2006 to December 2010.The last follow-up time was August 2017. The pathologic diagnosis of all cases was renal clear cell carcinoma after operation. This study was approved by the Ethics Committee of First affiliated Hospital of Gannan Medical University and written consent was collected from all patients.
RT-qPCR
Total RNA of renal cancer tissues and matched normal tissues was extracted with TRIzol (Invitrogen, USA) and treated with RNase-free DNase (Promega, USA). SYBR Green quantitative real-time PCR was performed by Stepone Real-time PCR system (Applied Biosystems 7000, USA). Beta-actin was used as the internal control. Relative expression level of each gene was calculated by the 2-DDCt method, in which ΔΔCt=Test group ΔCt (CT interested gene-CTBeta-actin) - Control group ΔCt (CT interested gene-CTBeta-actin).
Immunohistochemical analysis
Human tumor tissues were fixed in 4% paraformaldehyde, embedded in paraffin and sectioned as 5 μm. Each sectioned tissues were incubated with antibody against UBE2C (1:500, ab252940, Abcam, NY, USA) diluted with 0.01 M PBST buffer (0.01 M PBS, 0.001×Triton X-100)for 12 h at 44°C followed by HRP-conjugated secondary antibody (1:100, Beyotime, Shanghai, China). Peroxidase conjugates were determined using 3,3′-diaminobenzidine solution.
RNA interference assay (RNAi)
siRNA fragments targeting human UBE2C gene (siUBE2C: 5’- TCCTTTTTGTGATTTCTGTATAG-3’) was designed and synthesized. Random sequence was designed as negative control (NC). Then RCC typical cells 786-O cells were cultured in a 6-well plate and transduced with siUBE2C or NC (General Biosystems, Anhui, China) by Lipo reagent (Yeasen, Shanghai, China) for 4-6 h according to the manufacture’s instruction. The knockdown efficiency of siUBE2C was tested by RT-qPCR method.
Cell proliferation assay
Treated cells were seeded into a 96-well plate at 5×103 cell/well and cultured consecutively for 96 h. At each designed time point, 10 μL of CCK-8 agent was added and cultured for another 1-2 h. Then OD value at 450 nm was detected on microplate reader (Molecular Devices, CA, USA). The proliferation rate of cells was calculated according to the negative control.
Cell invasion assay
Transwell rooms with 8-μm-pore size membrane were pre-treated with 0.2 mL Matrigel 12 h before seeding tumor cells. Then a total of 2×104 cells/well was seeded into upper room and DMEM medium without FBS was added. DMEM medium containing15% FBS was added in the lower room. After culture for 24 h, cells on the upper room were removed and cells in the lower surface of upper room were fixed by 4% paraformaldehyde for 30 min and dyed with 0.5% crystal violet for 10 min. Then stained cells were observed under a microscope and counted.
Statistical analysis
SPSS 16.0software (SPSS, Inc., Chicago, IL, USA) was used to analyze the experimental data. All data was expressed as mean±SD. TCGA data were analyzed by the independent samples t-test to compare the differential levels of UBE2C mRNA between RCC and control. Chi-square test or Fisher’s exact test was employed to analyze the relationship between UBE2C expression and clinicopathological factors of RCC patients. Kaplan-Meier analysis was used to produce the survival curve. Univariate and Cox multivariate survival analyses were performed to analyze the prognostic significance of UBE2C in RCC patients. The difference was considered statistically significant when the p-value was less than 0.05.
Results
UBE2C was highly expressed in RCC
To explore the role of UBE2C in RCC, a total of 525 RCC cases and 72 normal cases from TCGA database (https://cancergenome. nih.gov/) were extracted for further analysis. The clinical information of the 525 RCC cases was displayed in Table 1. In brief, 187 cases were diagnosed as T3/T4 stage, accounted for 35.62% of total patients. About 205 cases were in stage Ⅲ/Ⅳ, accounted for 39.05%. And 157 cases were dead, accounted for 29.9%. The mRNA level of UBE2C was significantly higher in RCC tissues than the control (p<0.05) (Figure 1A). The mean level of UBE2C in RCC was 5 folds of that in normal control. Furthermore, the expression level of UBE2C in stage IV patients was remarkably higher than that in stage I patients (Figure 1B).
To verify the bioinformatics data, the mRNA expression of UBE2C in 90 of RCC tissues and matched control was determined by RT-qPCR method. In consistency, UBE2C was expressed much higher in tumor tissues than the control and immunohistochemical (IHC) analysis confirmed this result (Figure 1C and 1D). Therefore, UBE2C was over-expressed in RCC tissues.
UBE2C was correlated to clinicopathological factors in RCC patients
Tumor is a malignant disease caused by multiple factors and genetic gene is a key factor. Driver gene is a critical genetic factor in tumor. In this study, RCC patients were separated as low and high group based on the expression of UBE2C. The cut value is 2- fold (UBE2C expression in RCC tissues to control tissues). Then chi-square test was performed to analyze the relationship of UBE2C level with clinical factors in RCC patients. As shown in Table 2, UBE2C expression was associated with TNM stage, pathological stage, gender and survival but not age in RCC patients.16 Briefly, UBE2C was expressed at high level in 45.42% of T3 patients while at low level in 21.67% of T3 patients. But it was 38.93% vs 63.5% in T1 patients.
In stage I, 37.79% patients displayed high UBE2C expression when it was 62.36% in low UBE2C expression. But it was 29.01% vs 17.87% in stage III and 24.43% vs 6.84% in stage IV patients. Moreover, death rate of patients with high UBE2C expression is 41.98% but it was 17.87% in low UBE2C RCC patients. In 90 clinical cases, Clinicopathological analysis demonstrated that UBE2C was correlated to TNM stage and pathological stage in RCC (Table 3). Conclusively, UBE2C displayed important clinic role in progression of RCC.
UBE2C showed prognostic value in RCC
OS and progression-free survival (PFS) are two critical indicators in cancer. By mining TCGA database, we found that UBE2C expression was associated with OS and PFS in RCC patients. As shown in Figure 2A, RCC patients with higher UBE2C expression (n=262) showed much shorter OS than those with lower UBE2C expression (n=263) (p<0.05). And patients with lower UBE2C expression (n=262) displayed much better PFS than the control (Figure 2B, n=263) (p<0.05). By multivariate COX analysis, we found that patients with higher UBE2C expression exhibited worse OS than the control (p<0.001, HR=1.01, 95% CI 1.006-1.020) (Tables 3 and 4). In addition, pathological stage was associated with patient survival. Therefore, UBE2C might be a critical factor for predicting the prognosis of RCC patients.
Identification of potential signaling pathways associated with UBE2C in RCC
Based on the differential expressed genes (DEGs) extracted from TCGA database, we explored the signaling pathways involved in development of RCC by GO and KEGG analysis. As seen in Figure 3A and Table 5, GO analysis revealed that DEGs were mostly distributed in cell division (n=86), mitotic nuclear division (n=67), and sister chromatid cohesion (n=45) process. KEGG analysis revealed significance of UBE2C in about 17 signal pathways in which cell cycle was the most important pathway (p<0.001) (Figure 3B). About 40 DEGs were enriched in cell cycle (Table 6). Then a total of 13 DEGs was enriched in DNA replication and p53 signaling pathway (Figure 3B, Table 6). It is known to us that the signaling pathways including cell cycle, cell division, p53 signaling all play important roles in tumor. So it is conceived that UBE2C might regulate one or several of these pathways in RCC.
UBE2C is important for cell proliferation in RCC
To verify the real function of UBE2C in RCC, UBE2C was knocked down in 786-O cells. The knockdown efficiency of UBE2C in 786-O cells was over 66% (Figure 4A). Then by CCK- 8 assay, we found that the growth of 786-O cells was dramatically inhibited at third day of culture. The proliferation fold in UBE2C knockdown group was only 1.53 but it was 3.49 in the control (Figure 4B). Therefore, UBE2C was essential to the growth of RCC cells.
UBE2C contributes to cell migration in RCC
Tumor cells often have potent migration ability according to the previous study. In transwell assay, we found that 786-O cells couldn’t transmit through the chamber effectively. The number of transmitted cells in UBE2C knockdown group was decreased by 44% compared to the control (Figure 4 C,D). The results suggested that UBE2C conferred migration ability to 786-O cells.
Discussion
UBE2C is an important gene in cancer. For example, in prostate cancer, silence of UBE2C prevents cell growth.17-19 Methylation of UBE2C lead to gene instability.18 Over-expression of UBE2C is closely correlated to invasion and pathological stage in ovarian cancer.12 In this study, UBE2C was firstly demonstrated to be clinically associated with progression and prognosis in RCC patients. This was consistent with previous studies in other cancers such as ovarian, gastric cancer.12,19
UBE2C is also shown to be associated with poor prognosis in cancers.8,13,20,21In this study, we demonstrated that patients with high UBE2C expression displayed short survival time and decreased PFS, suggesting that UBE2C might be a prognostic factor in RCC. This was further supported by multivariate COX analysis, which suggested UBE2C as a critical gene in RCC. Taken together, UBE2C played critical roles in development and progression of RCC and might be a new biomarker for diagnosis or prognosis in advanced RCC.
RCC is a malignant disease affected by multiple factors and a series of signaling pathways contributed to the initiation or progression of RCC.22,23 In this study, the DEGs between tumor tissues and normal tissues were extracted from the TCGA databases followed by GO enrichment analysis. As stated above, the most correlated process was cell division in which 86 DEGs were enriched. Then 67 DEGs were enriched in mitotic nuclear division and 47 DEGs in DNA replication process. The three processes are known to play important roles in embryonic development orhomeostasis. 24,25 Aberrant in these processes often causes severe diseases or abnormalities such as cancer. So, it is possible that UBE2C might also regulate cell division, DNA replication, and mitotic nuclear division in RCC. In KEGG analysis, 40 DEGs was enriched in cell cycle pathway while 13 DEGs in DNA replication pathway. Accelerated cell division was common in cancer and was associated with unlimited cell proliferation and accelerated cell cycle. DNA replication was also an active process in cancer. Here, KEGG analysis showed that cell cycle and DNA replication were two top pathways in RCC. Indeed, active DNA replication process provides basis for cell cycle transition. This will further contribute to the division and proliferation of cancer cells. As a result, we deduce that UBE2C might regulate cell cycle and DNA replication pathway in RCC.
The bioinformatics analysis showed that UBE2C played important roles in clinic in RCC. This was further supported by in vitro assay in 786-O cells. As stated in the above, knockdown of UBE2C by RNNi significantly inhibited cell proliferation and migration in 786-O cells. It is known that potent proliferation and migration ability are two common traits in cancer.26,27 So, the preliminary data in 786-O cells further confirmed the role of UBE2C in RCC. However, more experiments are needed to support the oncogenic role of UBE2C in RCC and the mechanism that how UBE2C promotes progression in RCC. In future, we plan to further detect the role of UBE2C in more than two strain cell lines in RCC, especially the effects on cell apoptosis, cell cycle, and cell invasiveness. In addition, the in vivo role of UBE2C in RCC is a very important proof to support this study.
In summary, this study proves the clinical role of UBE2C and suggests it to be an important prognostic factor in RCC. UBE2C contributes to the proliferation and migration in RCC.
Abstract
Main Text
Introduction
Materials and Methods
Bioinformatics analysis and TCGA mining
RCC tissues and pathological information of patients
RT-qPCR
Immunohistochemical analysis
RNA interference assay (RNAi)
Cell proliferation assay
Cell invasion assay
Statistical analysis
Results
UBE2C was highly expressed in RCC
UBE2C was correlated to clinicopathological factors in RCC patients
UBE2C showed prognostic value in RCC
Identification of potential signaling pathways associated with UBE2C in RCC
UBE2C is important for cell proliferation in RCC
UBE2C contributes to cell migration in RCC
Discussion