Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells
The aim of this study was to explore the effects of the lncRNA ENST00000623984 on colorectal cancer. In this study, the expression levels of ENST000000623984 were first examined in tumor tissue and adjacent normal tissue from 40 patients with colorectal cancer and LoVo cells using quantitative real-time PCR. By siRNA transfection, ENST00000623984 expression was knocked down. Using flow cytometry, cell cycle progression and cell viability were examined in basal and knockdown LoVo cells. The CCK-8 assay was used to assess the cell proliferation rate, and the Transwell assay was used to determine the migration and invasion abilities. The ENST000000623984 expression level was increased in colorectal cancer. Knockdown of ENST000000623984 reduced cell viability, proliferation rate, cell migration and invasion. These results suggested that lncRNA ENST000000623984 may be involved in colorectal cancer development.
Torre LA, Bray F, Siegel RL, Ferlay J, Loetet-Tieulent J, Jemal A. Global cancer statistics. CA Cancer J Clin 2011;61:69-90.
Cech TR, Steitz JA. The noncoding RNA revolution-trashing old rules to forge new ones. Cell 2014;157:77-94.
Ling H, Fabbri M, Calin GA. MicroRNAs and other non-coding RNAs as targets for anticancer drug development. Nat Rev Drug Discov 2013;12:847-65.
Wang TH, Lin YS, Chen Y, Yeh CT, Huang YL, Hsieh TH, et al. Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition. Oncotarget 2015;6:23342-57.
Liu Y, Pan S, Liu L, Zhai X, Liu J, Wen J, et al. A genetic variant in long non-coding RNA HULC contributes to risk of HBV-related hepatocellular carcinoma in a Chinese population. PLoS One 2012;7:e35145.
Schonrock N, Harvey RP, Mattick JS. Long noncoding RNAs in cardiac development and pathophysiology. Circ Res 2012;111:1349-62.
Batista P, Chang H. Long noncoding RNAs: Cellular address codes in development and disease. Cell 2013;152:1298-307.
Faghihi MA, Modarresi F, Khalil AM, Wood DE, Sahagan BG, Morgan TE, et al. Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase. Nat Med 2008;14:723-30.
Li X, Wu Z, Fu X, Han W. Long noncoding RNAs: Insights from biological features and functions to diseases. Med Res Rev 2013;33:517-53.
Tan L, Yu JT, Hu N, Tan L. Non-coding RNAs in Alzheimer’s disease. Mol Neurobiol 2013;47:382-93.
Zhang X, Zhou Y, Mehta KR, Danila DC, Scolavino S, Johnson SR, et al. A pituitary-derived MEG3 isoform functions as a growth suppressor in tumor cells. J Clin Endocrinol Metab 2003;88:5119-26.
Wang TH, Yu CC, Lin YS, Chen TC, Yeh CT, Liang KH, et al. Long noncoding RNA CPS1-IT1 suppresses the metastasis of hepatocellular carcinoma by regulating HIF-1α activity and inhibiting epithelial-mesenchymal transition. Oncotarget 2016;7:43588-603.
Zhao XG, Liu MR, Zhang JJ, Zhang RS, Zhang Q, Tan XF. Long noncoding RNA CPS1-IT1 suppresses cell proliferation and metastasis in human lung cancer. Oncol Res 2017;25:373-80.
Hu L, Wu Y, Tan D, Meng H, Wang K, Bai Y, et al. Up-regulation of long noncoding RNA MALAT1 contributes to proliferation and metastasis in esophageal squamous cell carcinoma. J Exp Clin Cancer Res 2015;34:7.
Ma KX, Wang HJ, Li XR, Li T, Su G, Yang P, et al. Long noncoding RNA MALAT1 associates with the malignant status and poor prognosis in glioma. Tumour Biol 2015;36:3355-9.
Silva-Fisher JM, Dang HX, White NM, Strand MS, Krasnick BA, Rozycki EB, et al. Long non-coding RNA RAMS11 promotes metastatic colerectal cancr progression. Nat Commun 2020;11:2156.
Liu TY, Han ZY, Li HY, ZhuYK, Sun ZQ, Zhu AL. LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3. Mol Cancer 2018;17:118.
Tang R, Chen JH, Tang MT, Liao ZQ, Zhou LQ, Jiang JR, et al. LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in coloretal cancer. Int J Biol Sci 2019;15:2885-96.
Chen S, Liu Y, Wang YY, Xue ZP. LncRNA CCAT1 promotes colorectal cancer tumorigenesis via miR-181b-5p/TUSC3 axis. Onco Targets Ther 2019;12:9215-25.
Livak KJ and Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods 2001;25:402-8.
Prensner JR, Chinnaiyan AM. The emergence of lncRNAs in cancer biology. Cancer Discov 2011;1:391-407.
Liao Z, Nie H, Wang Y, Luo J, Zhou J, Ou C. The emerging landscape of long non-coding RNAs in colorectal cancer metastasis. Front Oncol 2021;11:641343.
Zhang J, Li K, Zheng H, Zhu Y. Research progress review on long non-coding RNA in colorectal cancer. Neoplasma 2021:68:240-52.
Talebi A, Azizpour M, Agah S, Masoodi M, Mobini GR, Akbari A. The relevance of long noncoding RNAs in colorectal cancer biology and clinical settings. J Cancer Res Ther 2020;16:S22-33.
Schwarzmueller L, Bril O, Vermeulen L, Léveillé N. Emerging role and therapeutic potential of lncRNAs in colorectal cancer. Cancers (Basel) 2020;12:3843.
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