https://doi.org/10.4081/ejh.2021.3215
Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells
Submitted: 30 December 2020
Accepted: 22 May 2021
Published: 12 July 2021
Accepted: 22 May 2021
Abstract Views: 1458
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The aim of this study was to explore the effects of the lncRNA ENST00000623984 on colorectal cancer. In this study, the expression levels of ENST000000623984 were first examined in tumor tissue and adjacent normal tissue from 40 patients with colorectal cancer and LoVo cells using quantitative real-time PCR. By siRNA transfection, ENST00000623984 expression was knocked down. Using flow cytometry, cell cycle progression and cell viability were examined in basal and knockdown LoVo cells. The CCK-8 assay was used to assess the cell proliferation rate, and the Transwell assay was used to determine the migration and invasion abilities. The ENST000000623984 expression level was increased in colorectal cancer. Knockdown of ENST000000623984 reduced cell viability, proliferation rate, cell migration and invasion. These results suggested that lncRNA ENST000000623984 may be involved in colorectal cancer development.
Torre LA, Bray F, Siegel RL, Ferlay J, Loetet-Tieulent J, Jemal A. Global cancer statistics. CA Cancer J Clin 2011;61:69-90.
DOI: https://doi.org/10.3322/caac.20107
Cech TR, Steitz JA. The noncoding RNA revolution-trashing old rules to forge new ones. Cell 2014;157:77-94.
DOI: https://doi.org/10.1016/j.cell.2014.03.008
Ling H, Fabbri M, Calin GA. MicroRNAs and other non-coding RNAs as targets for anticancer drug development. Nat Rev Drug Discov 2013;12:847-65.
DOI: https://doi.org/10.1038/nrd4140
Wang TH, Lin YS, Chen Y, Yeh CT, Huang YL, Hsieh TH, et al. Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition. Oncotarget 2015;6:23342-57.
DOI: https://doi.org/10.18632/oncotarget.4344
Liu Y, Pan S, Liu L, Zhai X, Liu J, Wen J, et al. A genetic variant in long non-coding RNA HULC contributes to risk of HBV-related hepatocellular carcinoma in a Chinese population. PLoS One 2012;7:e35145.
DOI: https://doi.org/10.1371/journal.pone.0035145
Schonrock N, Harvey RP, Mattick JS. Long noncoding RNAs in cardiac development and pathophysiology. Circ Res 2012;111:1349-62.
DOI: https://doi.org/10.1161/CIRCRESAHA.112.268953
Batista P, Chang H. Long noncoding RNAs: Cellular address codes in development and disease. Cell 2013;152:1298-307.
DOI: https://doi.org/10.1016/j.cell.2013.02.012
Faghihi MA, Modarresi F, Khalil AM, Wood DE, Sahagan BG, Morgan TE, et al. Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase. Nat Med 2008;14:723-30.
DOI: https://doi.org/10.1038/nm1784
Li X, Wu Z, Fu X, Han W. Long noncoding RNAs: Insights from biological features and functions to diseases. Med Res Rev 2013;33:517-53.
DOI: https://doi.org/10.1002/med.21254
Tan L, Yu JT, Hu N, Tan L. Non-coding RNAs in Alzheimer’s disease. Mol Neurobiol 2013;47:382-93.
DOI: https://doi.org/10.1007/s12035-012-8359-5
Zhang X, Zhou Y, Mehta KR, Danila DC, Scolavino S, Johnson SR, et al. A pituitary-derived MEG3 isoform functions as a growth suppressor in tumor cells. J Clin Endocrinol Metab 2003;88:5119-26.
DOI: https://doi.org/10.1210/jc.2003-030222
Wang TH, Yu CC, Lin YS, Chen TC, Yeh CT, Liang KH, et al. Long noncoding RNA CPS1-IT1 suppresses the metastasis of hepatocellular carcinoma by regulating HIF-1α activity and inhibiting epithelial-mesenchymal transition. Oncotarget 2016;7:43588-603.
DOI: https://doi.org/10.18632/oncotarget.9635
Zhao XG, Liu MR, Zhang JJ, Zhang RS, Zhang Q, Tan XF. Long noncoding RNA CPS1-IT1 suppresses cell proliferation and metastasis in human lung cancer. Oncol Res 2017;25:373-80.
DOI: https://doi.org/10.3727/096504016X14741486659473
Hu L, Wu Y, Tan D, Meng H, Wang K, Bai Y, et al. Up-regulation of long noncoding RNA MALAT1 contributes to proliferation and metastasis in esophageal squamous cell carcinoma. J Exp Clin Cancer Res 2015;34:7.
DOI: https://doi.org/10.1186/s13046-015-0123-z
Ma KX, Wang HJ, Li XR, Li T, Su G, Yang P, et al. Long noncoding RNA MALAT1 associates with the malignant status and poor prognosis in glioma. Tumour Biol 2015;36:3355-9.
DOI: https://doi.org/10.1007/s13277-014-2969-7
Silva-Fisher JM, Dang HX, White NM, Strand MS, Krasnick BA, Rozycki EB, et al. Long non-coding RNA RAMS11 promotes metastatic colerectal cancr progression. Nat Commun 2020;11:2156.
DOI: https://doi.org/10.1038/s41467-020-15547-8
Liu TY, Han ZY, Li HY, ZhuYK, Sun ZQ, Zhu AL. LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3. Mol Cancer 2018;17:118.
DOI: https://doi.org/10.1186/s12943-018-0873-2
Tang R, Chen JH, Tang MT, Liao ZQ, Zhou LQ, Jiang JR, et al. LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in coloretal cancer. Int J Biol Sci 2019;15:2885-96.
DOI: https://doi.org/10.7150/ijbs.38041
Chen S, Liu Y, Wang YY, Xue ZP. LncRNA CCAT1 promotes colorectal cancer tumorigenesis via miR-181b-5p/TUSC3 axis. Onco Targets Ther 2019;12:9215-25.
DOI: https://doi.org/10.2147/OTT.S216718
Livak KJ and Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods 2001;25:402-8.
DOI: https://doi.org/10.1006/meth.2001.1262
Prensner JR, Chinnaiyan AM. The emergence of lncRNAs in cancer biology. Cancer Discov 2011;1:391-407.
DOI: https://doi.org/10.1158/2159-8290.CD-11-0209
Liao Z, Nie H, Wang Y, Luo J, Zhou J, Ou C. The emerging landscape of long non-coding RNAs in colorectal cancer metastasis. Front Oncol 2021;11:641343.
DOI: https://doi.org/10.3389/fonc.2021.641343
Zhang J, Li K, Zheng H, Zhu Y. Research progress review on long non-coding RNA in colorectal cancer. Neoplasma 2021:68:240-52.
DOI: https://doi.org/10.4149/neo_2020_201012N1073
Talebi A, Azizpour M, Agah S, Masoodi M, Mobini GR, Akbari A. The relevance of long noncoding RNAs in colorectal cancer biology and clinical settings. J Cancer Res Ther 2020;16:S22-33.
DOI: https://doi.org/10.4103/jcrt.JCRT_327_18
Schwarzmueller L, Bril O, Vermeulen L, Léveillé N. Emerging role and therapeutic potential of lncRNAs in colorectal cancer. Cancers (Basel) 2020;12:3843.
DOI: https://doi.org/10.3390/cancers12123843
How to Cite
Liu, Z.- bao ., Zhang, J.- hua ., Gao, J.- hua ., & Shi, J. (2021). Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells. European Journal of Histochemistry, 65(3). https://doi.org/10.4081/ejh.2021.3215
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