https://doi.org/10.4081/ejh.2021.3215
Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells
Submitted: 30 December 2020
Accepted: 22 May 2021
Published: 12 July 2021
Accepted: 22 May 2021
Abstract Views: 1458
PDF: 408
HTML: 23
HTML: 23
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The aim of this study was to explore the effects of the lncRNA ENST00000623984 on colorectal cancer. In this study, the expression levels of ENST000000623984 were first examined in tumor tissue and adjacent normal tissue from 40 patients with colorectal cancer and LoVo cells using quantitative real-time PCR. By siRNA transfection, ENST00000623984 expression was knocked down. Using flow cytometry, cell cycle progression and cell viability were examined in basal and knockdown LoVo cells. The CCK-8 assay was used to assess the cell proliferation rate, and the Transwell assay was used to determine the migration and invasion abilities. The ENST000000623984 expression level was increased in colorectal cancer. Knockdown of ENST000000623984 reduced cell viability, proliferation rate, cell migration and invasion. These results suggested that lncRNA ENST000000623984 may be involved in colorectal cancer development.
Torre LA, Bray F, Siegel RL, Ferlay J, Loetet-Tieulent J, Jemal A. Global cancer statistics. CA Cancer J Clin 2011;61:69-90.
DOI: https://doi.org/10.3322/caac.20107
Cech TR, Steitz JA. The noncoding RNA revolution-trashing old rules to forge new ones. Cell 2014;157:77-94.
DOI: https://doi.org/10.1016/j.cell.2014.03.008
Ling H, Fabbri M, Calin GA. MicroRNAs and other non-coding RNAs as targets for anticancer drug development. Nat Rev Drug Discov 2013;12:847-65.
DOI: https://doi.org/10.1038/nrd4140
Wang TH, Lin YS, Chen Y, Yeh CT, Huang YL, Hsieh TH, et al. Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition. Oncotarget 2015;6:23342-57.
DOI: https://doi.org/10.18632/oncotarget.4344
Liu Y, Pan S, Liu L, Zhai X, Liu J, Wen J, et al. A genetic variant in long non-coding RNA HULC contributes to risk of HBV-related hepatocellular carcinoma in a Chinese population. PLoS One 2012;7:e35145.
DOI: https://doi.org/10.1371/journal.pone.0035145
Schonrock N, Harvey RP, Mattick JS. Long noncoding RNAs in cardiac development and pathophysiology. Circ Res 2012;111:1349-62.
DOI: https://doi.org/10.1161/CIRCRESAHA.112.268953
Batista P, Chang H. Long noncoding RNAs: Cellular address codes in development and disease. Cell 2013;152:1298-307.
DOI: https://doi.org/10.1016/j.cell.2013.02.012
Faghihi MA, Modarresi F, Khalil AM, Wood DE, Sahagan BG, Morgan TE, et al. Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid feed-forward regulation of beta-secretase. Nat Med 2008;14:723-30.
DOI: https://doi.org/10.1038/nm1784
Li X, Wu Z, Fu X, Han W. Long noncoding RNAs: Insights from biological features and functions to diseases. Med Res Rev 2013;33:517-53.
DOI: https://doi.org/10.1002/med.21254
Tan L, Yu JT, Hu N, Tan L. Non-coding RNAs in Alzheimer’s disease. Mol Neurobiol 2013;47:382-93.
DOI: https://doi.org/10.1007/s12035-012-8359-5
Zhang X, Zhou Y, Mehta KR, Danila DC, Scolavino S, Johnson SR, et al. A pituitary-derived MEG3 isoform functions as a growth suppressor in tumor cells. J Clin Endocrinol Metab 2003;88:5119-26.
DOI: https://doi.org/10.1210/jc.2003-030222
Wang TH, Yu CC, Lin YS, Chen TC, Yeh CT, Liang KH, et al. Long noncoding RNA CPS1-IT1 suppresses the metastasis of hepatocellular carcinoma by regulating HIF-1α activity and inhibiting epithelial-mesenchymal transition. Oncotarget 2016;7:43588-603.
DOI: https://doi.org/10.18632/oncotarget.9635
Zhao XG, Liu MR, Zhang JJ, Zhang RS, Zhang Q, Tan XF. Long noncoding RNA CPS1-IT1 suppresses cell proliferation and metastasis in human lung cancer. Oncol Res 2017;25:373-80.
DOI: https://doi.org/10.3727/096504016X14741486659473
Hu L, Wu Y, Tan D, Meng H, Wang K, Bai Y, et al. Up-regulation of long noncoding RNA MALAT1 contributes to proliferation and metastasis in esophageal squamous cell carcinoma. J Exp Clin Cancer Res 2015;34:7.
DOI: https://doi.org/10.1186/s13046-015-0123-z
Ma KX, Wang HJ, Li XR, Li T, Su G, Yang P, et al. Long noncoding RNA MALAT1 associates with the malignant status and poor prognosis in glioma. Tumour Biol 2015;36:3355-9.
DOI: https://doi.org/10.1007/s13277-014-2969-7
Silva-Fisher JM, Dang HX, White NM, Strand MS, Krasnick BA, Rozycki EB, et al. Long non-coding RNA RAMS11 promotes metastatic colerectal cancr progression. Nat Commun 2020;11:2156.
DOI: https://doi.org/10.1038/s41467-020-15547-8
Liu TY, Han ZY, Li HY, ZhuYK, Sun ZQ, Zhu AL. LncRNA DLEU1 contributes to colorectal cancer progression via activation of KPNA3. Mol Cancer 2018;17:118.
DOI: https://doi.org/10.1186/s12943-018-0873-2
Tang R, Chen JH, Tang MT, Liao ZQ, Zhou LQ, Jiang JR, et al. LncRNA SLCO4A1-AS1 predicts poor prognosis and promotes proliferation and metastasis via the EGFR/MAPK pathway in coloretal cancer. Int J Biol Sci 2019;15:2885-96.
DOI: https://doi.org/10.7150/ijbs.38041
Chen S, Liu Y, Wang YY, Xue ZP. LncRNA CCAT1 promotes colorectal cancer tumorigenesis via miR-181b-5p/TUSC3 axis. Onco Targets Ther 2019;12:9215-25.
DOI: https://doi.org/10.2147/OTT.S216718
Livak KJ and Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) method. Methods 2001;25:402-8.
DOI: https://doi.org/10.1006/meth.2001.1262
Prensner JR, Chinnaiyan AM. The emergence of lncRNAs in cancer biology. Cancer Discov 2011;1:391-407.
DOI: https://doi.org/10.1158/2159-8290.CD-11-0209
Liao Z, Nie H, Wang Y, Luo J, Zhou J, Ou C. The emerging landscape of long non-coding RNAs in colorectal cancer metastasis. Front Oncol 2021;11:641343.
DOI: https://doi.org/10.3389/fonc.2021.641343
Zhang J, Li K, Zheng H, Zhu Y. Research progress review on long non-coding RNA in colorectal cancer. Neoplasma 2021:68:240-52.
DOI: https://doi.org/10.4149/neo_2020_201012N1073
Talebi A, Azizpour M, Agah S, Masoodi M, Mobini GR, Akbari A. The relevance of long noncoding RNAs in colorectal cancer biology and clinical settings. J Cancer Res Ther 2020;16:S22-33.
DOI: https://doi.org/10.4103/jcrt.JCRT_327_18
Schwarzmueller L, Bril O, Vermeulen L, Léveillé N. Emerging role and therapeutic potential of lncRNAs in colorectal cancer. Cancers (Basel) 2020;12:3843.
DOI: https://doi.org/10.3390/cancers12123843
How to Cite
Liu, Z.- bao ., Zhang, J.- hua ., Gao, J.- hua ., & Shi, J. (2021). Effects of the lncRNA ENST00000623984 on colon cancer and the biological characteristics of colon cancer cells. European Journal of Histochemistry, 65(3). https://doi.org/10.4081/ejh.2021.3215
Copyright (c) 2021 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- C. A. X. Costa, K. N. O. de Brito, M. A. Gomes, M. V. Caliari, Histopathological and immunohistochemical study of the hepatic lesions experimentally induced by Entamoeba dispar , European Journal of Histochemistry: Vol. 54 No. 3 (2010)
- F. Boschi, M. Fontanella, L. Calderan, A. Sbarbati, Luminescence and fluorescence of essential oils. Fluorescence imaging in vivo of wild chamomile oil , European Journal of Histochemistry: Vol. 55 No. 2 (2011)
- G. Laguna Hernández, A.E. Brechú-Franco, I. De la Cruz-Chacón, A.R. González-Esquinca, Histochemical detection of acetogenins and storage molecules in the endosperm of Annona macroprophyllata Donn Sm. seeds , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- Giamaica Conti, Dario Bertossi, Elena Dai Prè, Chiara Cavallini, Maria Teresa Scupoli, Giulia Ricciardi, Pierpaolo Parnigotto, Yves Saban, Andrea Sbarbati, Pierfrancesco Nocini, Regenerative potential of the Bichat fat pad determined by the quantification of multilineage differentiating stress enduring cells , European Journal of Histochemistry: Vol. 62 No. 4 (2018)
- Enrica Cappellozza, Federico Boschi, Maddalena Sguizzato, Elisabetta Esposito, Rita Cortesi, Manuela Malatesta, Laura Calderan, A spectrofluorometric analysis to evaluate transcutaneous biodistribution of fluorescent nanoparticulate gel formulations , European Journal of Histochemistry: Vol. 66 No. 1 (2022)
- Hitoshi Fujita, Kosuke Imura, Masahito Takiguchi, Kengo Funakoshi, Postnatal development of thalamic reticular nucleus projections to the anterior thalamic nuclei in rats , European Journal of Histochemistry: Vol. 66 No. 2 (2022)
- S Bettini, F Ciani, V Franceschini, Cell proliferation and growth-associated protein 43 expression in the olfactory epithelium in Poecilia reticulata after copper solution exposure , European Journal of Histochemistry: Vol. 50 No. 2 (2006)
- C Squillacioti, A De Luca, G Paino, N Mirabella, Effects of castration on the immunoreactivity to NGF, BDNF and their receptors in the pelvic ganglia of the male rat , European Journal of Histochemistry: Vol. 52 No. 2 (2008)
- E Capelli, R Nano, S Panelli, L Sciola, A Spano, S Barni, Cytoskeleton actin changes in IL-2 activated cells , European Journal of Histochemistry: Vol. 44 No. 3 (2000)
- K Hirai, M Kumakiri, K Ueda, Y Imamura, S Noriki, Y Nishi, H Kato, M Fukuda, Clonal evolution and progression of 20-methylcholanthrene-induced squamous cell carcinoma of mouse epidermis as revealed by DNA instability and other malignancy markers , European Journal of Histochemistry: Vol. 45 No. 4 (2001)
<< < 61 62 63 64 65 66 67 > >>
You may also start an advanced similarity search for this article.
