https://doi.org/10.4081/ejh.2021.3220

Morphology, clearing efficacy, and mTOR dependency of the organelle autophagoproteasome

Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
Submitted: 15 January 2021
Accepted: 1 May 2021
Published: 1 June 2021
Autophagy, proteasome, LC3, Beclin 1, P20S, alpha-synuclein, transmission electron microscopy, confocal microscopy
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Authors

Fiona Limanaqi
https://orcid.org/0000-0003-0185-2099
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Italy.
Francesca Biagioni
https://orcid.org/0000-0003-3566-4889
IRCCS Neuromed, Pozzilli (IS), Italy.
Alessandra Salvetti
https://orcid.org/0000-0002-9154-9773
Department of Clinical and Experimental Medicine, University of Pisa, Italy.
Stefano Puglisi-Allegra
IRCCS Neuromed, Pozzilli (IS), Italy.
Paola Lenzi
https://orcid.org/0000-0002-4734-8798
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Italy.
Francesco Fornai
francesco.fornai@unipi.it
https://orcid.org/0000-0003-0185-2099
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa; IRCCS Neuromed, Pozzilli (IS), Italy.

The interplay between autophagy (ATG) and ubiquitin proteasome (UP) cell-clearing systems was recently evidenced at biochemical and morphological levels, where subunits belonging to both pathways co-localize within a novel organelle named autophagoproteasome (APP). We previously documented that APP occurs at baseline conditions, while it is hindered by neurotoxicant administration. This is bound to the activity of the mechanistic target of rapamycin (mTOR), since APP is stimulated by mTOR inhibition, which in turn, is correlated with cell protection. In this brief report, we provide novel, morphological and biochemical evidence on APP, suggesting the presence of active UP subunits within ATG vacuoles. Although a stream of interpretation considers such a merging as a catabolic pathway to clear inactive UP subunits, our data further indicate that UP-ATG merging may rather provide an empowered catalytic organelle.

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Supporting Agencies

Ministero della Salute (Ricerca Corrente)

How to Cite

Limanaqi, F., Biagioni, F. ., Salvetti, A. ., Puglisi-Allegra, S., Lenzi, P., & Fornai, F. . (2021). Morphology, clearing efficacy, and mTOR dependency of the organelle autophagoproteasome. European Journal of Histochemistry, 65(s1). https://doi.org/10.4081/ejh.2021.3220

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