Overexpression of MYBL2 predicts poor prognosis and promotes oncogenesis in endometrial carcinoma

  • Lulu Le Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Ji Luo Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Haifang Wu Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Ling Chen Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.
  • Xiaoli Tang College of Basic Medical Science, Nanchang University, Nanchang, Jiangxi Province, China.
  • Fen Fu | fu_fen@163.com Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China. https://orcid.org/0000-0002-7823-1816

Abstract

Endometrial cancer (EC) is the most common gynecologic malignancy and still remains clinically challenging. We aimed to explore the potential biomarkers of EC and provide a theoretical basis for early screening and targeted therapy. The available transcriptome data from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) were analyzed to identify differentially expressed genes. Immunohistochemistry was performed to detect gene expression. We analyzed the associations of MYBL2 with clinicopathological features and survival time and the biological effect of MYBL2 on the proliferation of EC cells. The effect of MYBL2 silencing on the transcriptome of EC cell model was analyzed by RNA-Seq. MYBL2 was significantly upregulated with obvious copy number alteration (CNA) in EC. Copy number amplification significantly increased MYBL2 mRNA expression, which led to a poor prognosis and severe pathological types of EC. Additionally, MYBL2 silencing significantly inhibited proliferation and induced apoptosis and G1-phase cell cycle arrest in EC cell lines. Our results indicate that MYBL2 is closely related to the cell cycle and apoptosis pathways in EC. The findings in this study provide evidence that MYBL2 can serve as a new candidate prognostic marker and a target for future therapeutic intervention in EC.

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References

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Published
2021-03-30
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Keywords:
Endometrial carcinoma, MYBL2, copy number, proliferation, prognosis
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How to Cite
Le, L., Luo, J., Wu, H., Chen, L., Tang, X., & Fu, F. (2021). Overexpression of <em>MYBL2</em&gt; predicts poor prognosis and promotes oncogenesis in endometrial carcinoma. European Journal of Histochemistry, 65(2). https://doi.org/10.4081/ejh.2021.3226