MicroRNA-29b-3p promotes 5-fluorouracil resistance via suppressing TRAF5-mediated necroptosis in human colorectal cancer

  • Shuimei Wu Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
  • Yun Zhou Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
  • Ping Liu Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
  • Hui Zhang Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
  • Wanliang Wang Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
  • Yuan Fang Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China.
  • Xiang Shen | hao350318178@outlook.com Department of Gastroenterology, Wuhu No.1 People’s Hospital, Wuhu City, China. https://orcid.org/0000-0003-0702-6847

Abstract

Drug resistance in colorectal cancer is a great challenge in clinic. Elucidating the deep mechanism underlying drug resistance will bring much benefit to diagnosis, therapy and prognosis in patients with colorectal cancer. In this study, miR-29b-3p was shown to be involved in resistance to 5-fluorouracil (5-FU)-induced necroptosis of colorectal cancer. Further, miR-29b-3p was shown to target a regulatory subunit of necroptosis TRAF5. Rescue of TRAF5 could reverse the effect of miR-29b-3p on 5-FU-induced necroptosis, which was consistent with the role ofnecrostatin-1 (a specific necroptosis inhibitor). Then it was demonstrated that miR-29b-3p was positively correlated with chemo-resistance in colorectal cancer while TRAF5 negatively. In conclusion, it is deduced that miR-29b-3p/TRAF5 signaling axis plays critical role in drug resistance in chemotherapy for colorectal cancer patients by regulating necroptosis. The findings in this study provide us a new target for interfere therapy in colorectal cancer.

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Published
2021-06-22
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Keywords:
colorectal cancer, miR-29-3p, TRAF5, necroptosis, 5-fluorouracil resistance
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How to Cite
Wu, S., Zhou, Y., Liu, P., Zhang, H., Wang, W., Fang, Y., & Shen, X. (2021). MicroRNA-29b-3p promotes 5-fluorouracil resistance <em>via</em&gt; suppressing TRAF5-mediated necroptosis in human colorectal cancer . European Journal of Histochemistry, 65(2). https://doi.org/10.4081/ejh.2021.3247