https://doi.org/10.4081/ejh.2021.3331
Mucin1 relieves acute lung injury by inhibiting inflammation and oxidative stress
Submitted: 19 September 2021
Accepted: 16 November 2021
Published: 2 December 2021
Accepted: 16 November 2021
Abstract Views: 1764
PDF: 1106
HTML: 25
HTML: 25
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is a kind of diffuse inflammatory injury caused by various factors, characterized by respiratory distress and progressive hypoxemia. It is a common clinical critical illness. The aim of this study was to investigate the effect and mechanism of the Mucin1 (MUC1) gene and its recombinant protein on lipopolysaccharide (LPS)-induced ALI/ARDS. We cultured human alveolar epithelial cell line (BEAS-2B) and used MUC1 overexpression lentivirus to detect the effect of MUC1 gene on BEAS-2B cells. In addition, we used LPS to induce ALI/ARDS in C57/BL6 mice and use hematoxylin and eosin (H&E) staining to verify the effect of their modeling. Recombinant MUC1 protein was injected subcutaneously into mice. We examined the effect of MUC1 on ALI/ARDS in mice by detecting the expression of inflammatory factors and oxidative stress molecules in mouse lung tissue, bronchoalveolar lavage fluid (BALF) and serum. Overexpression of MUC1 effectively ameliorated LPS-induced damage to BEAS-2B cells. Results of H&E staining indicate that LPS successfully induced ALI/ARDS in mice and MUC1 attenuated lung injury. MUC1 also reduced the expression of inflammatory factors (IL-1β, TNF-α, IL-6 and IL-8) and oxidative stress levels in mice. In addition, LPS results in an increase in the activity of the TLR4/NF-κB signaling pathway in mice, whereas MUC1 decreased the expression of the TLR4/NF-κB signaling pathway. MUC1 inhibited the activity of TLR4/NF-κB signaling pathway and reduced the level of inflammation and oxidative stress in lung tissue of ALI mice.
Han WZ, Xu SW, Wang L. Impact of ketamine intervention for acute lung injury on RAGE and TLR9. Eur Rev Med Pharmacol Sci 2018;22:4350-4.
Stormann P, Becker N, Vollrath JT, Kohler K, Janicova A, Wutzler S, et al. Early local inhibition of club cell protein 16 following chest trauma reduces late sepsis-induced acute lung injury. J Clin Med 2019;8:896.
DOI: https://doi.org/10.3390/jcm8060896
Wang L, Cao Y, Gorshkov B, Zhou Y, Yang Q, Xu J, et al. Ablation of endothelial Pfkfb3 protects mice from acute lung injury in LPS-induced endotoxemia. Pharmacol Res 2019;146:104292.
DOI: https://doi.org/10.1016/j.phrs.2019.104292
Lai WY, Wang JW, Huang BT, Lin EP, Yang PC. A novel TNF-alpha-targeting aptamer for TNF-alpha-mediated acute lung injury and acute liver failure. Theranostics 2019;9:1741-51.
DOI: https://doi.org/10.7150/thno.30972
Thomas G, Horwich A. Chemical strike against a dominant-inherited MUC1-frameshifted protein associated with progressive kidney disease. Trends Mol Med 2019;25:821-3.
DOI: https://doi.org/10.1016/j.molmed.2019.08.011
Shiba S, Miki A, Ohzawa H, Teratani T, Sakuma Y, Lefor AK, et al. Functional expression of Mucin1 in human duodenal adenocarcinoma. J Surg Res 2019;238:79-89.
DOI: https://doi.org/10.1016/j.jss.2019.01.006
Symmes BA, Stefanski AL, Magin CM, Evans CM. Role of mucins in lung homeostasis: regulated expression and biosynthesis in health and disease. Biochem Soc Trans 2018;46:707-19.
DOI: https://doi.org/10.1042/BST20170455
Wang YM, Qi X, Gong FC, Chen Y, Yang ZT, Mao EQ, et al. Protective and predictive role of Mucin1 in sepsis-induced ALI/ARDS. Int Immunopharmacol 2020;83:106438.
DOI: https://doi.org/10.1016/j.intimp.2020.106438
Wang YM, Ji R, Chen WW, Huang SW, Zheng YJ, Yang ZT, et al. Paclitaxel alleviated sepsis-induced acute lung injury by activating MUC1 and suppressing TLR-4/NF-kappaB pathway. Drug Des Devel Ther 2019;13:3391-404.
DOI: https://doi.org/10.2147/DDDT.S222296
Zhou H, Zhang Z, Liu G, Jiang M, Wang J, Liu Y, et al. The effect of different immunization cycles of a recombinant Mucin1-maltose-binding protein vaccine on T cell responses to B16-MUC1 melanoma in mice. Int J Mol Sci 2020;21
DOI: https://doi.org/10.3390/ijms21165810
Fu C, Hao S, Xu X, Zhou J, Liu Z, Lu H, et al. Activation of SIRT1 ameliorates LPS-induced lung injury in mice via decreasing endothelial tight junction permeability. Acta Pharmacol Sin 2019;40:630-41.
DOI: https://doi.org/10.1038/s41401-018-0045-3
Xiao K, He W, Guan W, Hou F, Yan P, Xu J, et al. Mesenchymal stem cells reverse EMT process through blocking the activation of NF-κB and Hedgehog pathways in LPS-induced acute lung injury. Cell Death Dis 2020;11:863.
DOI: https://doi.org/10.1038/s41419-020-03034-3
Wang F, Wang Y, Qu G, Yao X, Ma C, Song M, et al. Ultralong AgNWs-induced toxicity in A549 cells and the important roles of ROS and autophagy. Ecotoxicol Environ Saf 2019;186:109742.
DOI: https://doi.org/10.1016/j.ecoenv.2019.109742
Zhou J, Wu P, Sun H, Zhou H, Zhang Y, Xiao Z. Lung tissue extracellular matrix-derived hydrogels protect against radiation-induced lung injury by suppressing epithelial-mesenchymal transition. J Cell Physiol 2020;235:2377-88.
DOI: https://doi.org/10.1002/jcp.29143
Bueno M, Zank D, Buendia-Roldan I, Fiedler K, Mays BG, Alvarez D, et al. PINK1 attenuates mtDNA release in alveolar epithelial cells and TLR9 mediated profibrotic responses. Plos One 2019;14:e218003.
DOI: https://doi.org/10.1371/journal.pone.0218003
Li AL, Shen T, Wang T, Zhou MX, Wang B, Song JT, et al. Novel diterpenoid-type activators of the Keap1/Nrf2/ARE signaling pathway and their regulation of redox homeostasis. Free Radic Biol Med 2019;141:21-33.
DOI: https://doi.org/10.1016/j.freeradbiomed.2019.06.001
Wang J, Liu G, Li Q, Wang F, Xie F, Zhai R, et al. Mucin1 promotes the migration and invasion of hepatocellular carcinoma cells via JNK-mediated phosphorylation of Smad2 at the C-terminal and linker regions. Oncotarget 2015;6:19264-78.
DOI: https://doi.org/10.18632/oncotarget.4267
Liu B, Pan S, Xiao Y, Liu Q, Xu J, Jia L. LINC01296/miR-26a/GALNT3 axis contributes to colorectal cancer progression by regulating O-glycosylated MUC1 via PI3K/AKT pathway. J Exp Clin Cancer Res 2018;37:316.
DOI: https://doi.org/10.1186/s13046-018-0994-x
Li Q, Wang F, Liu G, Yuan H, Chen T, Wang J, et al. Impact of Mucin1 knockdown on the phenotypic characteristics of the human hepatocellular carcinoma cell line SMMC-7721. Oncol Rep 2014;31:2811-9.
DOI: https://doi.org/10.3892/or.2014.3136
Liu C, Tang X, Zhang W, Li G, Chen Y, Guo A, et al. 6-bromoindirubin-3'-oxime suppresses LPS-induced inflammation via inhibition of the TLR4/NF-kappaB and TLR4/MAPK signaling pathways. Inflammation 2019;42:2192-204.
DOI: https://doi.org/10.1007/s10753-019-01083-1
Yan S, Jiang Z, Cheng L, Lin Y, Fan B, Luo L, et al. TLR4 knockout can improve dysfunction of beta-cell by rebalancing proteomics disorders in pancreas of obese rats. Endocrine 2020;67:67-79.
DOI: https://doi.org/10.1007/s12020-019-02106-5
Zhang J, Zheng Y, Luo Y, Du Y, Zhang X, Fu J. Curcumin inhibits LPS-induced neuroinflammation by promoting microglial M2 polarization via TREM2/ TLR4/ NF-kappaB pathways in BV2 cells. Mol Immunol 2019;116:29-37.
DOI: https://doi.org/10.1016/j.molimm.2019.09.020
Baek CH, Kim H, Moon SY, Park SK, Yang WS. Epigallocatechin-3-gallate downregulates lipopolysaccharide signaling in human aortic endothelial cells by inducing ectodomain shedding of TLR4. Eur J Pharmacol 2019;863:172692.
DOI: https://doi.org/10.1016/j.ejphar.2019.172692
Ye R, Liu Z. ACE2 exhibits protective effects against LPS-induced acute lung injury in mice by inhibiting the LPS-TLR4 pathway. Exp Mol Pathol 2020;113:104350.
DOI: https://doi.org/10.1016/j.yexmp.2019.104350
Zhang ZM, Wang YC, Chen L, Li Z. Protective effects of the suppressed NF-kappaB/TLR4 signaling pathway on oxidative stress of lung tissue in rat with acute lung injury. Kaohsiung J Med Sci 2019;35:265-76.
DOI: https://doi.org/10.1002/kjm2.12065
Yang Y, Liu S, Liu J, Ta N. Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kbetasignaling. J Reprod Immunol 2020;142:103192.
DOI: https://doi.org/10.1016/j.jri.2020.103192
How to Cite
Ye, C. ., Xu, B. ., Yang, J. ., Liu, Y. ., Zeng, Z. ., Xia, L. ., … Zou, G. (2021). Mucin1 relieves acute lung injury by inhibiting inflammation and oxidative stress. European Journal of Histochemistry, 65(4). https://doi.org/10.4081/ejh.2021.3331
Copyright (c) 2021 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Qian Hu, Ran Tao, Xiaoyun Hu, Haibo Wu, Jianjun Xu, Effects of piperlonguminine on lung injury in severe acute pancreatitis via the TLR4/NF-κB pathway , European Journal of Histochemistry: Vol. 67 No. 2 (2023)
- Sabrina Giacoppo, Agnese Gugliandolo, Oriana Trubiani, Federica Pollastro, Gianpaolo Grassi, Placido Bramanti, Emanuela Mazzon, Cannabinoid CB2 receptors are involved in the protection of RAW264.7 macrophages against the oxidative stress: an in vitro study , European Journal of Histochemistry: Vol. 61 No. 1 (2017)
- M. Scimeca, A. Orlandi, I. Terrenato, S. Bischetti, E. Bonanno, Assessment of metal contaminants in non-small cell lung cancer by EDX microanalysis , European Journal of Histochemistry: Vol. 58 No. 3 (2014)
- Xiangjun Lu, Jian Shen, Siyuan Huang, Dongdong Liu, Haitao Wang, Tumor cells-derived exosomal PD-L1 promotes the growth and invasion of lung cancer cells in vitro via mediating macrophages M2 polarization , European Journal of Histochemistry: Vol. 67 No. 3 (2023)
- Zhijiang Chen, Huili Wang, Bin Hu, Xinxin Chen, Meiyu Zheng, Lili Liang, Juanjuan Lyu, Qiyi Zeng, Transcription factor nuclear factor erythroid 2 p45-related factor 2 (NRF2) ameliorates sepsis-associated acute kidney injury by maintaining mitochondrial homeostasis and improving the mitochondrial function , European Journal of Histochemistry: Vol. 66 No. 3 (2022)
- Yanmei Yao, Leqing Lin, Wenxue Tang, Yueliang Shen, Fayu Chen, Ning Li, Baiyong Wang, Pretreatment with geniposide mitigates myocardial ischemia/reperfusion injury by modulating inflammatory response through TLR4/NF-κB pathway , European Journal of Histochemistry: Vol. 67 No. 3 (2023)
- Liying Shen, Kongjie Lu, Zhenfeng Chen, Yingwei Zhu, Cong Zhang, Li Zhang, Pre-treatment with galectin-1 attenuates lipopolysaccharide-induced myocarditis by regulating the Nrf2 pathway , European Journal of Histochemistry: Vol. 67 No. 4 (2023)
- Y. Dong, C. Yang, Z. Wang, Z. Qin, J. Cao, Y. Chen, The injury of serotonin on intestinal epithelium cell renewal of weaned diarrhoea mice , European Journal of Histochemistry: Vol. 60 No. 4 (2016)
- R. Leonardi, R.E. Perrotta, C. Loreto, G. Musumeci, S. Crimi, J.N. dos Santos, M.C. Rusu, P. Bufo, E. Barbato, G. Pannone, Toll-like receptor 4 expression in the epithelium of inflammatory periapical lesions. An immunohistochemical study , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- Peipei Lu, Shuxiang Li, Caoyang Zhang, Xinyi Jiang, Jinghua Xiang, Hong Xu, Jian Dong, Kun Wang, Yuhua Shi, Spinosin ameliorates osteoarthritis through enhancing the Nrf2/HO-1 signaling pathway , European Journal of Histochemistry: Vol. 68 No. 2 (2024)
You may also start an advanced similarity search for this article.
