https://doi.org/10.4081/ejh.2022.3336
Inhibition of APLN suppresses cell proliferation and migration and promotes cell apoptosis in esophageal cancer cells in vitro, through activating PI3K/mTOR signaling pathway
Submitted: 1 October 2021
Accepted: 13 July 2022
Published: 3 August 2022
Accepted: 13 July 2022
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Esophageal cancer is the sixth leading cause of cancer mortalities globally with a high incidence rate. Apelin (APLN) plays regulatory roles in different organs. However, its role in esophageal cancer remains unknown. Therefore, our study aims to explore the effect of APLN on esophageal cancer. One hundred and eighty-four (184) esophageal tumor tissues samples from patients with esophageal cancer, and 11 esophageal tissues samples from healthy volunteers were analyzed for the expression of APLN. APLN was highly expressed in the tumor of patients with esophageal cancer and esophageal cancer cells. Patients with high expressions of APLN had a lower survival rate than the ones with low to medium expressions of APLN. Human esophageal carcinoma cell lines, TE-1 and ECA-109 cells were transfected with APLN siRNA to knockdown APLN, or transfected with pcDNA-APLN to overexpress APLN. Inhibition of APLN by siRNA-APLN reduced proliferative, migrative, and invasive abilities of esophageal cancer cells and promoted cell apoptosis, which could be all restored by pcDNA-APLN. Moreover, knocking down APLN by siRNA-APLN suppressed the PI3K/mTOR signaling pathway. These findings identify that APLN inhibition might ameliorate esophageal cancer through activating the PI3K/mTOR signaling pathway, thus APLN could be a potential target for esophageal cancer.
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Ethics Approval
This study was approved by the Ethic Committee of Shanghai Chest Hospital, Shanghai Jiaotong University, ChinaRights
National Natural Science Foundation of China; Three-year action plan for Shanghai to further accelerate the development of Chinese Medicine; third Round of Chinese Medicine Action Plan - Major Clinical Research Projects in Traditional Chinese Medicine; Interdisciplinary Program of Shanghai Jiao Tong UniversityHow to Cite
Wang, Y., Wang, G., Liu, X., Yun, D., Cui, Q., Wu, X., … Zhang, M. (2022). Inhibition of APLN suppresses cell proliferation and migration and promotes cell apoptosis in esophageal cancer cells <em>in vitro</em>, through activating PI3K/mTOR signaling pathway. European Journal of Histochemistry, 66(3). https://doi.org/10.4081/ejh.2022.3336
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