https://doi.org/10.4081/ejh.2022.3336
Inhibition of APLN suppresses cell proliferation and migration and promotes cell apoptosis in esophageal cancer cells in vitro, through activating PI3K/mTOR signaling pathway
Submitted: 1 October 2021
Accepted: 13 July 2022
Published: 3 August 2022
Accepted: 13 July 2022
Abstract Views: 834
PDF: 505
HTML: 20
HTML: 20
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Esophageal cancer is the sixth leading cause of cancer mortalities globally with a high incidence rate. Apelin (APLN) plays regulatory roles in different organs. However, its role in esophageal cancer remains unknown. Therefore, our study aims to explore the effect of APLN on esophageal cancer. One hundred and eighty-four (184) esophageal tumor tissues samples from patients with esophageal cancer, and 11 esophageal tissues samples from healthy volunteers were analyzed for the expression of APLN. APLN was highly expressed in the tumor of patients with esophageal cancer and esophageal cancer cells. Patients with high expressions of APLN had a lower survival rate than the ones with low to medium expressions of APLN. Human esophageal carcinoma cell lines, TE-1 and ECA-109 cells were transfected with APLN siRNA to knockdown APLN, or transfected with pcDNA-APLN to overexpress APLN. Inhibition of APLN by siRNA-APLN reduced proliferative, migrative, and invasive abilities of esophageal cancer cells and promoted cell apoptosis, which could be all restored by pcDNA-APLN. Moreover, knocking down APLN by siRNA-APLN suppressed the PI3K/mTOR signaling pathway. These findings identify that APLN inhibition might ameliorate esophageal cancer through activating the PI3K/mTOR signaling pathway, thus APLN could be a potential target for esophageal cancer.
Enzinger PC, Mayer RJ. Esophageal cancer. N Engl J Med 2003;349:2241-52.
DOI: https://doi.org/10.1056/NEJMra035010
Zhang Y. Epidemiology of esophageal cancer. World J Gastroenterol 2013;19:5598.
DOI: https://doi.org/10.3748/wjg.v19.i34.5598
O'Dowd BF, Heiber M, Chan A, Heng HH, Tsui LC, Kennedy JL, et al. A human gene that shows identity with the gene encoding the angiotensin receptor is located on chromosome 11. Gene 1993;136:355-60.
DOI: https://doi.org/10.1016/0378-1119(93)90495-O
Szokodi I, Tavi P, Földes G, Voutilainen-Myllylä S, Ilves M, Tokola H, et al. Apelin, the novel endogenous ligand of the orphan receptor APJ, regulates cardiac contractility. Circ Res 2002;91:434-40.
DOI: https://doi.org/10.1161/01.RES.0000033522.37861.69
Huang J, Koulaouzidis A, Marlicz W, Lok V, Chu C, Ngai CH, et al. Global burden, risk factors, and trends of esophageal cancer: An analysis of cancer registries from 48 countries. Cancers 2021;13:141.
DOI: https://doi.org/10.3390/cancers13010141
Wong MC, Hamilton W, Whiteman DC, Jiang JY, Qiao Y, Fung FD, et al. Global Incidence and mortality of oesophageal cancer and their correlation with socioeconomic indicators temporal patterns and trends in 41 countries. Sci Rep 2018;8:1-13.
DOI: https://doi.org/10.1038/s41598-018-19819-8
Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 2012;380:2095-128.
DOI: https://doi.org/10.1016/S0140-6736(12)61728-0
Lee DK, Cheng R, Nguyen T, Fan T, Kariyawasam AP, Liu Y, et al. Characterization of apelin, the ligand for the APJ receptor. J Neurochem 2000;74:34-41.
DOI: https://doi.org/10.1046/j.1471-4159.2000.0740034.x
Antushevich H, Wójcik M. Review: Apelin in disease. Clin Chim Acta 2018;483:241-8.
DOI: https://doi.org/10.1016/j.cca.2018.05.012
Chen H, Wong CC, Liu D, Go MYY, Wu B, Peng S, et al. APLN promotes hepatocellular carcinoma through activating PI3K/Akt pathway and is a druggable target. Theranostics 2019;9:5246-60.
DOI: https://doi.org/10.7150/thno.34713
Liu Z, Iyer MR, Godlewski G, Jourdan T, Liu J, Coffey NJ, et al. Functional selectivity of a biased cannabinoid-1 receptor (CB(1)R) antagonist. ACS Pharmacol Transl Sci 2021;4:1175-87.
DOI: https://doi.org/10.1021/acsptsci.1c00048
Ma Y, Yu S, Zhao W, Lu Z, Chen J. miR-27a regulates the growth, colony formation and migration of pancreatic cancer cells by targeting Sprouty2. Cancer Lett 2010;298:150-8.
DOI: https://doi.org/10.1016/j.canlet.2010.06.012
Liu Z, Luo H, Zhang L, Huang Y, Liu B, Ma K, et al. Hyperhomocysteinemia exaggerates adventitial inflammation and angiotensin II-induced abdominal aortic aneurysm in mice. Circ Res 2012;111:1261-73.
DOI: https://doi.org/10.1161/CIRCRESAHA.112.270520
Chen S, Cheng A-C, Wang M-S, Peng X. Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling. World J Gastroenterol 2008;14:2174.
DOI: https://doi.org/10.3748/wjg.14.2174
Yang Y, Lv SY, Ye W, Zhang L. Apelin/APJ system and cancer. Clin Chim Acta 2016;457:112-6.
DOI: https://doi.org/10.1016/j.cca.2016.04.001
Chen Y, Lin X, Zheng J, Chen J, Xue H, Zheng X. APLN: A potential novel biomarker for cervical cancer. Sci Prog 2021;104:368504211011341.
DOI: https://doi.org/10.1177/00368504211011341
Masoumi J, Jafarzadeh A, Khorramdelazad H, Abbasloui M, Abdolalizadeh J, Jamali N. Role of apelin/APJ axis in cancer development and progression. Adv Med Sci 2020;65:202-13.
DOI: https://doi.org/10.1016/j.advms.2020.02.002
Ethics Approval
This study was approved by the Ethic Committee of Shanghai Chest Hospital, Shanghai Jiaotong University, ChinaRights
National Natural Science Foundation of China; Three-year action plan for Shanghai to further accelerate the development of Chinese Medicine; third Round of Chinese Medicine Action Plan - Major Clinical Research Projects in Traditional Chinese Medicine; Interdisciplinary Program of Shanghai Jiao Tong UniversityHow to Cite
Wang, Y., Wang, G., Liu, X., Yun, D., Cui, Q., Wu, X., … Zhang, M. (2022). Inhibition of APLN suppresses cell proliferation and migration and promotes cell apoptosis in esophageal cancer cells <em>in vitro</em>, through activating PI3K/mTOR signaling pathway. European Journal of Histochemistry, 66(3). https://doi.org/10.4081/ejh.2022.3336
Copyright (c) 2022 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- W Zhao, H Shen, F Yuan, G Li, Y Sun, Z Shi, Y Zhang, Z Wang, Induction stage-dependent expression of vascular endothelial growth factor and aquaporin-1 in diethylstilbestrol-treated rat pituitary , European Journal of Histochemistry: Vol. 53 No. 1 (2009)
- E. I. Cortés-Gutiérrez, M. I. Davila-RodrÃguez, J. L. Fernández, C. López-Fernández, J. Gosalvez, DNA breakage detection-fluorescence in situ hybridization (DBD-FISH) in buccal cells , European Journal of Histochemistry: Vol. 56 No. 4 (2012)
- Li Niu, Xiaoying Chu, Yaofei Jiang, Wei Zeng, HPV infection upregulates the expression of ZNT-1 in condyloma acuminatum , European Journal of Histochemistry: Vol. 65 No. 2 (2021)
- AI Scovassi, A Torriglia, Activation of DNA-degrading enzymes during apoptosis , European Journal of Histochemistry: Vol. 47 No. 3 (2003)
- E Bonanno, M Ruzittu, EC Carlà , MR Montinari, P Pagliara, L Dini, Cell shape and organelle modification in apoptotic U937 cells , European Journal of Histochemistry: Vol. 44 No. 3 (2000)
- A. Mangas, J. Yajeya, N. González, I. Ruiz, M. Geffard, R. Coveñas, 3-hydroxi-anthranilic acid is early expressed in stroke , European Journal of Histochemistry: Vol. 60 No. 4 (2016)
- Francesca Bianco, Giulia Lattanzio, Luca Lorenzini, Chiara Diquigiovanni, Maurizio Mazzoni, Paolo Clavenzani, Laura Calzà, Luciana Giardino, Catia Sternini, Elena Bonora, Roberto De Giorgio, Novel understanding on genetic mechanisms of enteric neuropathies leading to severe gut dysmotility , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
- J. Zhang, J. Luo, J. Ni, L. Tang, H.P. Zhang, L. Zhang, J.F. Xu, D. Zheng, Erratum - MMP-7 is upregulated by COX-2 and promotes proliferation and invasion of lung adenocarcinoma cells , European Journal of Histochemistry: Vol. 68 No. 3 (2024)
- F. Dorko, D. Kluchová, A. Boleková, K. Lovasová, T. Špakovská, T. Borošová, Influence of surgical and chemical orchidectomy on weight and distribution of AChE-nerve fibres in thymuses of adult rats , European Journal of Histochemistry: Vol. 55 No. 3 (2011)
- C. Dall'Oca, T. Maluta, F. Cavani, G.P. Morbioli, P. Bernardi, A. Sbarbati, D. Degl'Innocenti, B. Magnan, The biocompatibility of porous vs non-porous bone cements: a new methodological approach , European Journal of Histochemistry: Vol. 58 No. 2 (2014)
<< < 39 40 41 42 43 44 45 46 47 48 > >>
You may also start an advanced similarity search for this article.
