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Autofluorescence in freshly isolated adult human liver sinusoidal cells

Authors

Anett Kristin Larsen
anett.k.larsen@uit.no
Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø, Norway.
Jaione Simón-Santamaría
Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø, Norway.
Kjetil Elvevold
Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø, Norway.
Bo Göran Ericzon
Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska University Hospital, Stockholm, Sweden.
Kim Erlend Mortensen
University Hospital of North Norway, Tromsø, Norway.
Peter McCourt
Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø, Norway.
Bård Smedsrød
Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø, Norway.
Karen Kristine Sørensen
Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø, Norway.

Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that lysosomally located autofluorescent material reflected accumulation of lipofuscin in the intact liver. These results illustrate the importance of careful selection of fluorophores, especially when labelling of live cells where the quencher is not compatible.

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Supporting Agencies

Norwegian Research Council
Kjetil Elvevold, Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø

Current address:
The Norwegian Institute of Food, Fisheries and Aquaculture Research, Tromsø, Norway

How to Cite

Larsen, A. K., Simón-Santamaría, J., Elvevold, K., Ericzon, B. G. ., Mortensen, K. E., McCourt, P., … Sørensen, K. K. (2021). Autofluorescence in freshly isolated adult human liver sinusoidal cells. European Journal of Histochemistry, 65(4). https://doi.org/10.4081/ejh.2021.3337
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