Autofluorescence in freshly isolated adult human liver sinusoidal cells

Lysosomal labelling with mouse anti-human LAMP-1 antibody (green) shows that the autofluorescent material (red) is located within LAMP-1 positive vesicles (arrow heads)
Submitted: 5 October 2021
Accepted: 1 December 2021
Published: 13 December 2021
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Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that lysosomally located autofluorescent material reflected accumulation of lipofuscin in the intact liver. These results illustrate the importance of careful selection of fluorophores, especially when labelling of live cells where the quencher is not compatible.

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Supporting Agencies

Norwegian Research Council
Kjetil Elvevold, Department of Medical Biology, UiT – The Arctic University of Norway, Tromsø

Current address:
The Norwegian Institute of Food, Fisheries and Aquaculture Research, Tromsø, Norway

How to Cite

Larsen, A. K., Simón-Santamaría, J., Elvevold, K., Ericzon, B. G. ., Mortensen, K. E., McCourt, P., … Sørensen, K. K. (2021). Autofluorescence in freshly isolated adult human liver sinusoidal cells. European Journal of Histochemistry, 65(4). https://doi.org/10.4081/ejh.2021.3337

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