https://doi.org/10.4081/ejh.2022.3342

MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS

Vol. 66 No. 2 (2022)
Submitted: 12 October 2021
Accepted: 9 May 2022
Published: 31 May 2022
Atherosclerosis, ox-LDL, miR-130a-5p, FAS
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Authors

Wei Wang
The First Clinical College of Jinan University, Guangzhou, and Surgical Oncology Department, the Second Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Wenbo Tang
Vascular Surgery Department, the First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Erbo Shan
General Surgery Department, the Second Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Lei Zhang
Surgical Oncology Department, the Second Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Shiyuan Chen
Vascular Surgery Department, the First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Chaowen Yu
Vascular Surgery Department, the First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Yong Gao
0000731@bbmc.edu.cn
https://orcid.org/0000-0003-3694-2094
The First Clinical College of Jinan University, Guangzhou, China.

MicroRNAs (miRNAs) play critical roles in the development of vascular diseases. However, the effects of miR-130a-5p and its functional targets on atherosclerosis (AS) are still largely unknown. In this regard, our aim is to explore the potentially important role of miR-130a-5p and its target gene during the progression of endothelial cell injury. We first found oxidized low-density lipoprotein (ox-LDL) induced FAS and cell apoptosis in HUVECs. Subsequently, miR-130a-5p expression was verified to be downregulated after ox-LDL treatment and negatively correlated with FAS, and FAS was identified as substantially upregulated in the ox-LDL-treated HUVEC cells. After that, the knockdown of FAS and overexpression of miR-130a-5p together were observed to aggregate ox-LDL-induced reduction of cell viability and apoptosis, cell cycle progression, cell proliferation, cell migration and invasion. In conclusion, we detected that miR-130a-5p contributed to the progression of endothelial cell injury by regulating of FAS, which may provide a new and promising therapeutic target for AS.

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How to Cite

Wang, W., Tang, W., Shan, E., Zhang, L., Chen, S., Yu, C., & Gao, Y. (2022). MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS. European Journal of Histochemistry, 66(2). https://doi.org/10.4081/ejh.2022.3342
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