https://doi.org/10.4081/ejh.2022.3342
MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS
Submitted: 12 October 2021
Accepted: 9 May 2022
Published: 31 May 2022
Accepted: 9 May 2022
Abstract Views: 689
PDF: 474
HTML: 23
HTML: 23
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
MicroRNAs (miRNAs) play critical roles in the development of vascular diseases. However, the effects of miR-130a-5p and its functional targets on atherosclerosis (AS) are still largely unknown. In this regard, our aim is to explore the potentially important role of miR-130a-5p and its target gene during the progression of endothelial cell injury. We first found oxidized low-density lipoprotein (ox-LDL) induced FAS and cell apoptosis in HUVECs. Subsequently, miR-130a-5p expression was verified to be downregulated after ox-LDL treatment and negatively correlated with FAS, and FAS was identified as substantially upregulated in the ox-LDL-treated HUVEC cells. After that, the knockdown of FAS and overexpression of miR-130a-5p together were observed to aggregate ox-LDL-induced reduction of cell viability and apoptosis, cell cycle progression, cell proliferation, cell migration and invasion. In conclusion, we detected that miR-130a-5p contributed to the progression of endothelial cell injury by regulating of FAS, which may provide a new and promising therapeutic target for AS.
Malakar AK, Choudhury D, Halder B, Paul P, Uddin A, Chakraborty S. A review on coronary artery disease, its risk factors, and therapeutics. J Cell Physiol 2019;234:16812-23.
DOI: https://doi.org/10.1002/jcp.28350
Peng N, Meng N, Wang S, Zhao F, Zhao J, Su L, et al. An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E(-)/(-) mice. Sci Rep 2014;4:5519.
DOI: https://doi.org/10.1038/srep05519
Rahman MS, Woollard K. Atherosclerosis. Adv Exp Med Biol 2017;1003:121-44.
DOI: https://doi.org/10.1007/978-3-319-57613-8_7
Suciu CF, Prete M, Ruscitti P, Favoino E, Giacomelli R, Perosa F. Oxidized low density lipoproteins: The bridge between atherosclerosis and autoimmunity. Possible implications in accelerated atherosclerosis and for immune intervention in autoimmune rheumatic disorders. Autoimmun Rev 2018;17:366-75.
DOI: https://doi.org/10.1016/j.autrev.2017.11.028
Pedicino D, Giglio AF, Galiffa VA, Cialdella P, Trotta F, Graziani F, et al. Infections, immunity and atherosclerosis: pathogenic mechanisms and unsolved questions. Int J Cardiol 2013;166:572-83.
DOI: https://doi.org/10.1016/j.ijcard.2012.05.098
Trpkovic A, Resanovic I, Stanimirovic J, Radak D, Mousa SA, Cenic-Milosevic D, et al. Oxidized low-density lipoprotein as a biomarker of cardiovascular diseases. Crit Rev Clin Lab Sci 2015;52:70-85.
DOI: https://doi.org/10.3109/10408363.2014.992063
Wang R, Zhang Y, Xu L, Lin Y, Yang X, Bai L, et al. Protein inhibitor of activated STAT3 Suppresses oxidized LDL-induced cell responses during atherosclerosis in apolipoprotein E-deficient mice. Sci Rep. 2016;6:36790.
DOI: https://doi.org/10.1038/srep36790
Ambros V. The functions of animal microRNAs. Nature 2004;431:350-5.
DOI: https://doi.org/10.1038/nature02871
Si W, Shen J, Zheng H, Fan W. The role and mechanisms of action of microRNAs in cancer drug resistance. Clin Epigenetics 2019;11:25.
DOI: https://doi.org/10.1186/s13148-018-0587-8
Sun D, Xiang G, Wang J, Li Y, Mei S, Ding H, et al. miRNA 146b-5p protects against atherosclerosis by inhibiting vascular smooth muscle cell proliferation and migration. Epigenomics 2020;12:2189-204.
DOI: https://doi.org/10.2217/epi-2020-0155
Zhang F, Cheng N, Du J, Zhang H, Zhang C. MicroRNA-200b-3p promotes endothelial cell apoptosis by targeting HDAC4 in atherosclerosis. BMC Cardiovasc Disord 2021;21:172.
DOI: https://doi.org/10.1186/s12872-021-01980-0
Liu F, Liu Y, Du Y, Li Y. MiRNA-130a promotes inflammation to accelerate atherosclerosis via the regulation of proliferator-activated receptor gamma (PPARgamma) expression. Anatol J Cardiol 2021;25:630-7.
DOI: https://doi.org/10.5152/AnatolJCardiol.2021.56721
Youssef EM, Elfiky AM, BanglySoliman, Abu-Shahba N, Elhefnawi MM. Expression profiling and analysis of some miRNAs in subcutaneous white adipose tissue during development of obesity. Genes Nutr 2020;15:8.
DOI: https://doi.org/10.1186/s12263-020-00666-0
Mo JS, Alam KJ, Kang IH, Park WC, Seo GS, Choi SC, et al. MicroRNA 196B regulates FAS-mediated apoptosis in colorectal cancer cells. Oncotarget 2015;6:2843-55.
DOI: https://doi.org/10.18632/oncotarget.3066
Fouque A, Debure L, Legembre P. The CD95/CD95L signaling pathway: a role in carcinogenesis. Biochim Biophys Acta 2014;1846:130-41.
DOI: https://doi.org/10.1016/j.bbcan.2014.04.007
Kischkel FC, Hellbardt S, Behrmann I, Germer M, Pawlita M, Krammer PH, et al. Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor. EMBO J 1995;14:5579-88.
DOI: https://doi.org/10.1002/j.1460-2075.1995.tb00245.x
Luo L, Liang H, Liu L. Myristicin regulates proliferation and apoptosis in oxidized low-density lipoprotein-stimulated human vascular smooth muscle cells and human umbilical vein endothelial cells by regulating the PI3K/Akt/NF-kappaB signalling pathway. Pharm Biol 2022;60:56-64.
DOI: https://doi.org/10.1080/13880209.2021.2010775
Yin Q, Fischer L, Noethling C, Schaefer WR. In vitro-assessment of putative antiprogestin activities of phytochemicals and synthetic UV absorbers in human endometrial Ishikawa cells. Gynecol Endocrinol 2015;31:578-81.
DOI: https://doi.org/10.3109/09513590.2015.1047448
Arsic N, Bendris N, Peter M, Begon-Pescia C, Rebouissou C, Gadea G, et al. A novel function for Cyclin A2: control of cell invasion via RhoA signaling. J Cell Biol 2012;196:147-62.
DOI: https://doi.org/10.1083/jcb.201102085
Chen LM, Wang W, Lee JC, Chiu FH, Wu CT, Tai CJ, et al. Thrombomodulin mediates the progression of epithelial ovarian cancer cells. Tumour Biol 2013;34:3743-51.
DOI: https://doi.org/10.1007/s13277-013-0958-x
Espada J, Matabuena M, Salazar N, Lucena S, Kourani O, Carrasco E, et al. Cryptomphalus aspersa mollusc eggs extract promotes migration and prevents cutaneous ageing in keratinocytes and dermal fibroblasts in vitro. Int J Cosmet Sci 2015;37:41-55.
DOI: https://doi.org/10.1111/ics.12167
Lee SJ, Kim WJ, Moon SK. Role of the p38 MAPK signaling pathway in mediating interleukin-28A-induced migration of UMUC-3 cells. Int J Mol Med 2012;30:945-52.
DOI: https://doi.org/10.3892/ijmm.2012.1064
Xian X, Tang L, Wu C, Huang L. miR-23b-3p and miR-130a-5p affect cell growth, migration and invasion by targeting CB1R via the Wnt/beta-catenin signaling pathway in gastric carcinoma. Onco Targets Ther 2018;11:7503-12.
DOI: https://doi.org/10.2147/OTT.S181706
Ma F, Xie Y, Lei Y, Kuang Z, Liu X. The microRNA-130a-5p/RUNX2/STK32A network modulates tumor invasive and metastatic potential in non-small cell lung cancer. BMC Cancer. 2020;20:580.
DOI: https://doi.org/10.1186/s12885-020-07056-0
Liu YT, Liu GQ, Huang JM. FAM225A promotes sorafenib resistance in hepatocarcinoma cells through modulating miR-130a-5p-CCNG1 interaction network. Biosci Rep 2020;40: BSR20202054.
DOI: https://doi.org/10.1042/BSR20202054
Huang X, Xiao S, Zhu X, Yu Y, Cao M, Zhang X, et al. miR-196b-5p-mediated downregulation of FAS promotes NSCLC progression by activating IL6-STAT3 signaling. Cell Death Dis 2020;11:785.
DOI: https://doi.org/10.1038/s41419-020-02997-7
Su YF, Zang YF, Wang YH, Ding YL. MiR-19-3p induces tumor cell apoptosis via targeting FAS in rectal cancer cells. Technol Cancer Res Treat 2020;19:1533033820917978.
DOI: https://doi.org/10.1177/1533033820917978
Bai B, Yang Y, Ji S, Wang S, Peng X, Tian C, et al. MicroRNA-302c-3p inhibits endothelial cell pyroptosis via directly targeting NOD-, LRR- and pyrin domain-containing protein 3 in atherosclerosis. J Cell Mol Med 2021;25:4373-86.
DOI: https://doi.org/10.1111/jcmm.16500
Cheng Y, Zhou M, Zhou W. MicroRNA-30e regulates TGF-beta-mediated NADPH oxidase 4-dependent oxidative stress by Snai1 in atherosclerosis. Int J Mol Med 2019;43:1806-16.
DOI: https://doi.org/10.3892/ijmm.2019.4102
How to Cite
Wang, W., Tang, W., Shan, E., Zhang, L., Chen, S., Yu, C., & Gao, Y. (2022). MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS. European Journal of Histochemistry, 66(2). https://doi.org/10.4081/ejh.2022.3342
Copyright (c) 2022 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- C. Loreto, A. Psaila, G. Musumeci, S. Castorina, R. Leonardi, Apoptosis activation in human carious dentin. An immunohistochemical study , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- A. Vetuschi, A. D'Alfonso, R. Sferra, D. Zanelli, S. Pompili, F. Patacchiola, E. Gaudio, G. Carta, Changes in muscularis propria of anterior vaginal wall in women with pelvic organ prolapse , European Journal of Histochemistry: Vol. 60 No. 1 (2016)
- Carlo Pellicciari, Histochemistry in biology and medicine: a message from the citing journals , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- J.M. Ou, Z.Y. Yu, M.K. Qiu, Y.X. Dai, Q. Dong, J. Shen, X.F. Wang, Y.B. Liu, Z.W. Quan, Z.W. Fei, Knockdown of VEGFR2 inhibits proliferation and induces apoptosis in hemangioma-derived endothelial cells , European Journal of Histochemistry: Vol. 58 No. 1 (2014)
- C. Pellicciari, On the future contents of a small journal of histochemistry , European Journal of Histochemistry: Vol. 56 No. 4 (2012)
- Sara Salucci, Sabrina Burattini, Francesca Buontempo, Alberto Maria Martelli, Elisabetta Falcieri, Michela Battistelli, Protective effect of different antioxidant agents in UVB-irradiated keratinocytes , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
- Francesca Diomede, Soundara Rajan Thangavelu, Ilaria Merciaro, Monica D'Orazio, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani, Porphyromonas gingivalis lipopolysaccharide stimulation in human periodontal ligament stem cells: role of epigenetic modifications to the inflammation , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
- Elena Casiraghi, Mara Cossa, Veronica Huber, Licia Rivoltini, Matteo Tozzi, Antonello Villa, Barbara Vergani, MIAQuant, a novel system for automatic segmentation, measurement, and localization comparison of different biomarkers from serialized histological slices , European Journal of Histochemistry: Vol. 61 No. 4 (2017)
- Manuel Scimeca, Simone Bischetti, Harpreet Kaur Lamsira, Rita Bonfiglio, Elena Bonanno, Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- C. Loreto, R. Leonardi, G. Musumeci, G. Pannone, S. Castorina, An ex vivo study on immunohistochemical localization of MMP-7 and MMP-9 in temporomandibular joint discs with internal derangement , European Journal of Histochemistry: Vol. 57 No. 2 (2013)
You may also start an advanced similarity search for this article.
