https://doi.org/10.4081/ejh.2022.3364
How to stain nucleic acids and proteins in Miller spreads
Submitted: 30 November 2021
Accepted: 12 February 2022
Published: 25 February 2022
Accepted: 12 February 2022
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The spread technique proposed by Miller and Beatty in 1969 allowed for the first time the visualization at transmission electron microscopy of nucleic acids and chromatin in an isolated and distended conformation. The final step of staining the spread chromatin is of critical importance because it can strongly influence the interpretation of the results. We evaluated different staining techniques and the most part of them provided a good result. Specifically, well contrasted micrographs were obtained when staining with H3PW12O40 (PTA), as originally proposed by Miller and Beatty, and with two alternatives proposed here: uranyl acetate or terbium citrate staining. Quite good contrast of the spread DNA could be achieved also by using Osmium Ammine; while no or few contrast of nucleic acids was observed by staining with KMnO₄ and H3PMo12O40 (PMA) respectively.
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How to Cite
Zannino, L. ., & Biggiogera, M. (2022). How to stain nucleic acids and proteins in Miller spreads. European Journal of Histochemistry, 66(1). https://doi.org/10.4081/ejh.2022.3364
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