https://doi.org/10.4081/ejh.2022.3477
Overexpression of hsa_circ_0006470 inhibits the malignant behavior of gastric cancer cells via regulation of miR-1234/TP53I11 axis
Submitted: 4 July 2022
Accepted: 9 September 2022
Published: 3 October 2022
Accepted: 9 September 2022
Abstract Views: 1030
PDF: 460
HTML: 24
HTML: 24
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Gastric cancer (GC) is a subtype of a common malignant tumor found in the digestive system. Hsa_circ_0006470 is known to be closely associated with the development of GC. Nevertheless, the mechanism by which hsa_circ_0006470 regulates the tumorigenesis of GC has not been fully elucidated. To investigate the role of hsa_circ_0006470 in GC, its expression levels were assessed in GES-1, AGS, MKN45, and SNU5 cells by reverse transcription-quantitative PCR. Fluorescence in situ hybridization was used to evaluate the localization of hsa_circ_0006470 in AGS and MKN45 cells. In addition, cell counting kit-8 and 5-ethynyl-2’-deoxyuridine assays were performed to evaluate the viability and proliferation of GC cells, respectively. The dual-luciferase reporter assay was used to explore the interaction among hsa_circ_0006470, microRNA (miR)-1234, and TP53I11. The expression levels of TP53I11, Akt, p-Akt, forkhead box O1, and cyclin dependent kinase 2 in AGS cells were analyzed by Western blotting. The data indicated that hsa_circ_0006470 expression was downregulated in AGS cells. In addition, overexpression (OE) of hsa_circ_0006470 could inhibit the viability and proliferation of GC cells. Moreover, OE of hsa_circ_0006470 inhibited the migration of GC cells and induced G1 cell cycle phase arrest. Moreover, miR-1234 was bound to hsa_circ_0006470 and TP53I11 was targeted by miR-1234. Furthermore, OE of hsa_circ_0006470 inhibited the tumorigenesis of GC via the regulation of the miR-1234/TP53I11 axis. In summary, the present study demonstrated that OE of hsa_circ_0006470 notably inhibited the tumorigenesis of GC by regulating the miR-1234/TP53I11 axis. Therefore, the present study may provide a theoretical basis for exploring novel therapeutic strategies for the treatment of GC.
Song Z, Wu Y, Yang J, Yang D, Fang X. Progress in the treatment of advanced gastric cancer. Tumour Biol 2017;39:1010428317714626.
DOI: https://doi.org/10.1177/1010428317714626
Somi MH, Mousavi SM, Naghashi S, Faramarzi E, Jafarabadi MA, Ghojazade M, et al. Is there any relationship between food habits in the last two decades and gastric cancer in North-Western Iran? Asian Pac J Cancer Prev 2015;16:283-90.
DOI: https://doi.org/10.7314/APJCP.2015.16.1.283
Amieva M, Peek RM, Jr. Pathobiology of Helicobacter pylori-induced gastric cancer. Gastroenterology 2016;150:64-78.
DOI: https://doi.org/10.1053/j.gastro.2015.09.004
Karimi P, Islami F, Anandasabapathy S, Freedman ND, Kamangar F. Gastric cancer: descriptive epidemiology, risk factors, screening, and prevention. Cancer Epidemiol Biomarkers Prev 2014;23:700-13.
DOI: https://doi.org/10.1158/1055-9965.EPI-13-1057
Tan Z. Recent advances in the surgical treatment of advanced gastric cancer: A review. Med Sci Monit 2019;25:3537-41.
DOI: https://doi.org/10.12659/MSM.916475
Religioni U, Czerw A, Badowska-Kozakiewicz AM, Deptała A. Assessment of pain, acceptance of illness, adjustment to life, and strategies of coping with illness among patients with gastric cancer. J Cancer Educ 2020;35:724-30.
DOI: https://doi.org/10.1007/s13187-019-01519-0
Ma X, Sun S, Zhao Y, Wang X, Meng W, Pang Z, et al. Impact of pain care and hospice care on quality of life in patients with advanced gastric cancer. Am J Transl Res 2021;13:8235-40.
Smyth EC, Nilsson M, Grabsch HI, van Grieken NC, Lordick F. Gastric cancer. Lancet 2020;396:635-48.
DOI: https://doi.org/10.1016/S0140-6736(20)31288-5
Zhou WY, Cai ZR, Liu J, Wang DS, Ju HQ, Xu RH. Circular RNA: metabolism, functions and interactions with proteins. Mol Cancer 2020;19:172.
DOI: https://doi.org/10.1186/s12943-020-01286-3
Li X, Li C, Liu Z, Ni W, Yao R, Xu Y, et al. Circular RNA circ-FoxO3 inhibits myoblast cells differentiation. Cells 2019;8:616. Retracted Article in Cells 2020;9:2504.
DOI: https://doi.org/10.3390/cells9112504
Vo JN, Cieslik M, Zhang Y, Shukla S, Xiao L, Zhang Y, et al. The landscape of circular RNA in cancer. Cel. 2019;176:869-81.e13.
DOI: https://doi.org/10.1016/j.cell.2018.12.021
Zhou Z, Sun B, Huang S, Zhao L. Roles of circular RNAs in immune regulation and autoimmune diseases. Cell Death Dis 2019;10:503.
DOI: https://doi.org/10.1038/s41419-019-1744-5
Kristensen LS, Andersen MS, Stagsted LVW, Ebbesen KK, Hansen TB, Kjems J. The biogenesis, biology and characterization of circular RNAs. Nat Rev Genet 2019;20:675-91.
DOI: https://doi.org/10.1038/s41576-019-0158-7
Hong X, Liu N, Liang Y, He Q, Yang X, Lei Y, et al. Circular RNA CRIM1 functions as a ceRNA to promote nasopharyngeal carcinoma metastasis and docetaxel chemoresistance through upregulating FOXQ1. Mol Cancer 2020;19:33.
DOI: https://doi.org/10.1186/s12943-020-01149-x
Zhang X, Wang S, Wang H, Cao J, Huang X, Chen Z, et al. Circular RNA circNRIP1 acts as a microRNA-149-5p sponge to promote gastric cancer progression via the AKT1/mTOR pathway. Mol Cancer 2019;18:20.
DOI: https://doi.org/10.1186/s12943-018-0935-5
Lu Q, Liu T, Feng H, Yang R, Zhao X, Chen W, et al. Circular RNA circSLC8A1 acts as a sponge of miR-130b/miR-494 in suppressing bladder cancer progression via regulating PTEN. Mol Cancer.2019;18:111.
DOI: https://doi.org/10.1186/s12943-019-1040-0
Shen Z, Zhou L, Zhang C, Xu J. Reduction of circular RNA Foxo3 promotes prostate cancer progression and chemoresistance to docetaxel. Cancer Lett 2020;468:88-101.
DOI: https://doi.org/10.1016/j.canlet.2019.10.006
Chen LY, Wang L, Ren YX, Pang Z, Liu Y, Sun XD, et al. The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation. Mol Cancer 2020;19:164.
DOI: https://doi.org/10.1186/s12943-020-01272-9
Ma C, Wang X, Yang F, Zang Y, Liu J, Wang X, et al. Circular RNA hsa_circ_0004872 inhibits gastric cancer progression via the miR-224/Smad4/ADAR1 successive regulatory circuit. Mol Cancer 2020;19:157.
DOI: https://doi.org/10.1186/s12943-020-01268-5
Peng L, Sang H, Wei S, Li Y, Jin D, Zhu X, et al. circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2. Mol Cancer 2020;19:156.
DOI: https://doi.org/10.1186/s12943-020-01270-x
Yao L, Xie Y. Down-regulation of hsa_circ_0006470 predicts tumor invasion: A new biomarker of gastric cancer. J Clin Lab Ana. 2021;35:e23879.
DOI: https://doi.org/10.1002/jcla.23879
Wong LS, Wei L, Wang GC, Law CT, Tsang HC, Chin WC, et al. In vivo genome-wide CRISPR activation screening identifies functionally important long non-coding RNAs in hepatocellular carcinoma. Cell Mol Gastroenterol Hepatol 2022. Online Ahead of Print.
DOI: https://doi.org/10.1016/j.jcmgh.2022.07.017
Dong L, Deng J, Sun ZM, Pan AP, Xiang XJ, Zhang L, et al. Interference with the β-catenin gene in gastric cancer induces changes to the miRNA expression profile. Tumour Biol 2015;36:6973-83.
DOI: https://doi.org/10.1007/s13277-015-3415-1
Wang L, Huang J, Jiang M, Lin H. Tissue-specific transplantation antigen P35B (TSTA3) immune response-mediated metabolism coupling cell cycle to postreplication repair network in no-tumor hepatitis/cirrhotic tissues (HBV or HCV infection) by biocomputation. Immunol Res 2012;52:258-68.
DOI: https://doi.org/10.1007/s12026-012-8337-z
Guggenheim DE, Shah MA. Gastric cancer epidemiology and risk factors. J Surg Oncol 2013;107:230-6.
DOI: https://doi.org/10.1002/jso.23262
Li R, Jiang J, Shi H, Qian H, Zhang X, Xu W. CircRNA: a rising star in gastric cancer. Cell Mol Life Sci 2020;77:1661-80.
DOI: https://doi.org/10.1007/s00018-019-03345-5
Cui Y, Cao J, Huang S, Ye J, Huang H, Liao D, et al. circRNA_0006470 promotes the proliferation and migration of gastric cancer cells by functioning as a sponge of miR-27b-3p. Neoplasma 2021;68:1245-56.
DOI: https://doi.org/10.4149/neo_2021_210222N235
Xie M, Yu T, Jing X, Ma L, Fan Y, Yang F, et al. Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation. Mol Cancer 2020;19:112.
DOI: https://doi.org/10.1186/s12943-020-01208-3
Luo Z, Rong Z, Zhang J, Zhu Z, Yu Z, Li T, et al. Circular RNA circCCDC9 acts as a miR-6792-3p sponge to suppress the progression of gastric cancer through regulating CAV1 expression. Mol Cancer 2020;19:86.
DOI: https://doi.org/10.1186/s12943-020-01203-8
Xiao T, Xu Z, Zhou Y, Zhang H, Geng J, Liang Y, et al. Loss of TP53I11 enhances the extracellular matrix-independent survival by promoting activation of AMPK. IUBMB Life 2019;71:183-91.
DOI: https://doi.org/10.1002/iub.1949
Ethics Approval
The Ethical Committee of Shanghai Jiading District Central Hospital approved this study (No. SHJDCH20220407)Supporting Agencies
This work was supported by Project of Jiading District Science and Technology Commission (JDKW- 2019-w01)How to Cite
Xie, J. ., Ning, Y. ., Zhang, L. ., Lin, Y. ., Guo, R., & Wang, S. . (2022). Overexpression of hsa_circ_0006470 inhibits the malignant behavior of gastric cancer cells <em>via</em> regulation of miR-1234/TP53I11 axis. European Journal of Histochemistry, 66(4). https://doi.org/10.4081/ejh.2022.3477
Copyright (c) 2022 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- G Silvestrini, P Mocetti, R Di Grezia, S Berni, E Bonucci, Localization of the glucocorticoid receptor mRNA in cartilage and bone cells of the rat. An in situ hybridization study , European Journal of Histochemistry: Vol. 47 No. 3 (2003)
- Shule Hou, Jiarui Chen, Jun Yang, Autophagy precedes apoptosis during degeneration of the Kölliker’s organ in the development of rat cochlea , European Journal of Histochemistry: Vol. 63 No. 2 (2019)
- Michael Williams Leal Quirino, Michelly Cristiny Pereira, Maria de Fátima Deodato de Souza, Ivan da Rocha Pitta, Antônio Felix da Silva Filho, Mario S. de Souza Albuquerque, Amanda Pinheiro de Barros Albuquerque, Mário Rino Martins, Maira Galdino da Rocha Pitta, Moacyr Jesus Barreto de Melo Rêgo, Immunopositivity for Siglec-15 in gastric cancer and its association with clinical and pathological parameters , European Journal of Histochemistry: Vol. 65 No. 1 (2021)
- Ennio Pannese, Quantitative, structural and molecular changes in neuroglia of aging mammals: A review , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
- N Sjakste, T Sjakste, Possible involvement of DNA strand breaks in regulation of cell differentiation , European Journal of Histochemistry: Vol. 51 No. 2 (2007)
You may also start an advanced similarity search for this article.
