https://doi.org/10.4081/ejh.2022.3505
Polydatin reverses oxidation low lipoprotein (oxLDL)-induced apoptosis of human umbilical vein endothelial cells via regulating the miR-26a-5p/BID axis
Submitted: 27 July 2022
Accepted: 9 August 2022
Published: 20 September 2022
Accepted: 9 August 2022
Abstract Views: 774
PDF: 405
HTML: 32
HTML: 32
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Atherosclerosis is a disease in which lipids and inflammatory factors accumulate on the walls of arteries, forming plaques that eventually block the flow of blood. Polydatin was derived from plant knotweed, which could play an important role in inhibiting the progression of atherosclerosis. However, the mechanism by which polydatin regulates the genesis and development of atherosclerosis remains unclear. To detect the function of polydatin in atherosclerosis, the proliferation, apoptosis and migration of human umbilical vein endothelial cells (HUVECs) was detected using 5-ethynyl-2’-deoxyuridine staining, flow cytometry and transwell assays, respectively. In addition, the branch points and capillary length of HUVECs were observed using a tube formation assay, and the lipid accumulation was tested by Oil-red O staining assay. Dual luciferase reporter assays were performed to confirm the association between microRNA (miR)-26a-5p and BH3 interacting domain death agonist (BID) in HUVECs. The data suggested oxidized low-density lipoprotein (oxLDL) notably inhibited the viability of HUVECs in a dose-dependent manner, and polydatin reversed the oxLDL-induced inhibition of HUVECs viability and proliferation. In addition, polydatin inhibited the apoptosis, migration and epithelial mesenchymal transition (EMT) process in oxLDL-treated HUVECs. Polydatin reversed oxLDL-induced lipid accumulation and angiogenesis inhibition in HUVECs. Furthermore, BID was targeted by miR-26a-5p, and polydatin reversed the oxLDL-induced apoptosis of HUVECs via regulating the miR-26a-5p/BID axis. In summary, polydatin reversed the oxLDL-induced apoptosis of HUVECs via regulating the miR-26a-5p/BID axis. Therefore, polydatin could act as a new agent for atherosclerosis treatment.
Bennett MR, Sinha S, Owens GK. Vascular Smooth Muscle Cells in Atherosclerosis. Circulation research. 2016;118:692-702.
DOI: https://doi.org/10.1161/CIRCRESAHA.115.306361
Gimbrone MA, Jr., García-Cardeña G. Vascular endothelium, hemodynamics, and the pathobiology of atherosclerosis. Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology. 2013;22:9-15.
DOI: https://doi.org/10.1016/j.carpath.2012.06.006
Gimbrone MA, Jr., García-Cardeña G. Endothelial Cell Dysfunction and the Pathobiology of Atherosclerosis. Circulation research. 2016;118:620-36.
DOI: https://doi.org/10.1161/CIRCRESAHA.115.306301
Yang Q, Xu J, Ma Q, Liu Z, Sudhahar V, Cao Y, et al. PRKAA1/AMPKα1-driven glycolysis in endothelial cells exposed to disturbed flow protects against atherosclerosis. Nature communications. 2018;9:4667.
DOI: https://doi.org/10.1038/s41467-018-07132-x
Wolf D, Ley K. Immunity and Inflammation in Atherosclerosis. Circulation research. 2019;124:315-27.
DOI: https://doi.org/10.1161/CIRCRESAHA.118.313591
Santos MG, Pegoraro M, Sandrini F, Macuco EC. Risk factors for the development of atherosclerosis in childhood and adolescence. Arquivos brasileiros de cardiologia. 2008;90:276-83.
DOI: https://doi.org/10.1590/S0066-782X2008000400012
Libby P, Buring JE, Badimon L, Hansson GK, Deanfield J, Bittencourt MS, et al. Atherosclerosis. Nature reviews Disease primers. 2019;5:56.
DOI: https://doi.org/10.1038/s41572-019-0106-z
Dai T, He W, Yao C, Ma X, Ren W, Mai Y, et al. Applications of inorganic nanoparticles in the diagnosis and therapy of atherosclerosis. Biomaterials science. 2020;8:3784-99.
DOI: https://doi.org/10.1039/D0BM00196A
Wu M, Li X, Wang S, Yang S, Zhao R, Xing Y, et al. Polydatin for treating atherosclerotic diseases: A functional and mechanistic overview. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2020;128:110308.
DOI: https://doi.org/10.1016/j.biopha.2020.110308
Wang D, Luo X, Wang M, Zhou K, Xia Z. Selective separation and purification of polydatin by molecularly imprinted polymers from the extract of Polygoni Cuspidati Rhizoma et Radix, rats' plasma and urine. Journal of chromatography B, Analytical technologies in the biomedical and life sciences. 2020;1156:122307.
DOI: https://doi.org/10.1016/j.jchromb.2020.122307
Peng Y, Xu J, Zeng Y, Chen L, Xu XL. Polydatin attenuates atherosclerosis in apolipoprotein E-deficient mice: Role of reverse cholesterol transport. Phytomedicine : international journal of phytotherapy and phytopharmacology. 2019;62:152935.
DOI: https://doi.org/10.1016/j.phymed.2019.152935
Ma Y, Gong X, Mo Y, Wu S. Polydatin inhibits the oxidative stress-induced proliferation of vascular smooth muscle cells by activating the eNOS/SIRT1 pathway. International journal of molecular medicine. 2016;37:1652-60.
DOI: https://doi.org/10.3892/ijmm.2016.2554
Chen L, Heikkinen L, Wang C, Yang Y, Sun H, Wong G. Trends in the development of miRNA bioinformatics tools. Briefings in bioinformatics. 2019;20:1836-52.
DOI: https://doi.org/10.1093/bib/bby054
Tiwari A, Mukherjee B, Dixit M. MicroRNA Key to Angiogenesis Regulation: MiRNA Biology and Therapy. Current cancer drug targets. 2018;18:266-77.
DOI: https://doi.org/10.2174/1568009617666170630142725
Fabris L, Ceder Y, Chinnaiyan AM, Jenster GW, Sorensen KD, Tomlins S, et al. The Potential of MicroRNAs as Prostate Cancer Biomarkers. European urology. 2016;70:312-22.
DOI: https://doi.org/10.1016/j.eururo.2015.12.054
Lu Y, Thavarajah T, Gu W, Cai J, Xu Q. Impact of miRNA in Atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology. 2018;38:e159-e70.
DOI: https://doi.org/10.1161/ATVBAHA.118.310227
Xing X, Li Z, Yang X, Li M, Liu C, Pang Y, et al. Adipose-derived mesenchymal stem cells-derived exosome-mediated microRNA-342-5p protects endothelial cells against atherosclerosis. Aging. 2020;12:3880-98.
DOI: https://doi.org/10.18632/aging.102857
Jing R, Zhong QQ, Long TY, Pan W, Qian ZX. Downregulated miRNA-26a-5p induces the apoptosis of endothelial cells in coronary heart disease by inhibiting PI3K/AKT pathway. European review for medical and pharmacological sciences. 2019;23:4940-7.
Hu C, Bai X, Liu C, Hu Z. Long noncoding RNA XIST participates hypoxia-induced angiogenesis in human brain microvascular endothelial cells through regulating miR-485/SOX7 axis. American journal of translational research. 2019;11:6487-97.
Ren M, Wang T, Han Z, Fu P, Liu Z, Ouyang C. Long Noncoding RNA OIP5-AS1 Contributes to the Progression of Atherosclerosis by Targeting miR-26a-5p Through the AKT/NF-κB Pathway. Journal of cardiovascular pharmacology. 2020;76:635-44.
DOI: https://doi.org/10.1097/FJC.0000000000000889
Liu Y, Liu N, Liu Q. Constructing a ceRNA-immunoregulatory network associated with the development and prognosis of human atherosclerosis through weighted gene co-expression network analysis. Aging. 2021;13:3080-100.
DOI: https://doi.org/10.18632/aging.202486
Swirski FK, Nahrendorf M. Leukocyte behavior in atherosclerosis, myocardial infarction, and heart failure. Science (New York, NY). 2013;339:161-6.
DOI: https://doi.org/10.1126/science.1230719
Yamamoto N, Satomi J, Yamamoto Y, Shono K, Kanematsu Y, Izumi Y, et al. Risk Factors of Neurological Deterioration in Patients with Cerebral Infarction due to Large-Artery Atherosclerosis. Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association. 2017;26:1801-6.
DOI: https://doi.org/10.1016/j.jstrokecerebrovasdis.2017.04.011
Jia Z, An L, Lu Y, Xu C, Wang S, Wang J, et al. Oxidized Low Density Lipoprotein-Induced Atherogenic Response of Human Umbilical Vascular Endothelial Cells (HUVECs) was Protected by Atorvastatin by Regulating miR-26a-5p/Phosphatase and Tensin Homolog (PTEN). Medical science monitor : international medical journal of experimental and clinical research. 2019;25:9836-43.
DOI: https://doi.org/10.12659/MSM.918405
Zhong X, Zhang L, Li Y, Li P, Li J, Cheng G. Kaempferol alleviates ox-LDL-induced apoptosis by up-regulation of miR-26a-5p via inhibiting TLR4/NF-κB pathway in human endothelial cells. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2018;108:1783-9.
DOI: https://doi.org/10.1016/j.biopha.2018.09.175
How to Cite
Wang, D. ., Zhou, Z. ., & Yuan, L. (2022). Polydatin reverses oxidation low lipoprotein (oxLDL)-induced apoptosis of human umbilical vein endothelial cells <em>via</em> regulating the miR-26a-5p/BID axis. European Journal of Histochemistry, 66(4). https://doi.org/10.4081/ejh.2022.3505
Copyright (c) 2022 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- M.L. Escobar Sánchez, O.M. EcheverrÃa MartÃnez, G.H. Vázquez-Nin, Immunohistochemical and ultrastructural visualization of different routes of oocyte elimination in adult rats , European Journal of Histochemistry: Vol. 56 No. 2 (2012)
- N.P.E. Kadoglou, P. Moustardas, A. Kapelouzou, M. Katsimpoulas, A. Giagini, E. Dede, N. Kostomitsopoulos, P.E. Karayannacos, A. Kostakis, C.D. Liapis, The anti-inflammatory effects of exercise training promote atherosclerotic plaque stabilization in apolipoprotein E knockout mice with diabetic atherosclerosis , European Journal of Histochemistry: Vol. 57 No. 1 (2013)
- Shiyuan Chen, Longfei Zhang, Benchi Feng, Wei Wang, Delang Liu, Xinyu Zhao, Chaowen Yu, Xiaogao Wang, Yong Gao, MiR-550a-3p restores damaged vascular smooth muscle cells by inhibiting thrombomodulin in an in vitro atherosclerosis model , European Journal of Histochemistry: Vol. 66 No. 3 (2022)
- Meiying Zhang, Min Zhou, Xia Cai, Yan Zhou, Xueling Jiang, Yan Luo, Yue Hu, Rong Qiu, Yanrong Wu, Yuejin Zhang, Yan Xiong, VEGF promotes diabetic retinopathy by upregulating the PKC/ET/NF-κB/ICAM-1 signaling pathway , European Journal of Histochemistry: Vol. 66 No. 4 (2022)
- P. Donizy, A. Halon, P. Surowiak, G. Pietrzyk, C. Kozyra, R. Matkowski, Correlation between PARP-1 immunoreactivity and cytomorphological features of parthanatos, a specific cellular death in breast cancer cells , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- Wei Wang, Wenbo Tang, Erbo Shan, Lei Zhang, Shiyuan Chen, Chaowen Yu, Yong Gao, MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS , European Journal of Histochemistry: Vol. 66 No. 2 (2022)
- Antonio Monaco, Teresa Capriello, Maria C. Grimaldi, Valentina Schiano, Ida Ferrandino, Neurodegeneration in zebrafish embryos and adults after cadmium exposure , European Journal of Histochemistry: Vol. 61 No. 4 (2017)
- Sara Salucci, Sabrina Burattini, Francesca Buontempo, Alberto Maria Martelli, Elisabetta Falcieri, Michela Battistelli, Protective effect of different antioxidant agents in UVB-irradiated keratinocytes , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
- M.L. Escobar, O.M. EcheverrÃa, G. GarcÃa, R. OrtÃz, G.H. Vázquez-Nin, Immunohistochemical and ultrastructural study of the lamellae of oocytes in atretic follicles in relation to different processes of cell death , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- M. Chiusa, F. Timolati, J.C. Perriard, T.M. Suter, C. Zuppinger, Sodium nitroprusside induces cell death and cytoskeleton degradation in adult rat cardiomyocytes in vitro: implications for anthracycline-induced cardiotoxicity , European Journal of Histochemistry: Vol. 56 No. 2 (2012)
You may also start an advanced similarity search for this article.
