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SerpinB3 administration protects liver against ischemia-reperfusion injury
We have investigated the change in SerpinB3 during hepatic ischemia and the potential role of its antiprotease activity in cell protection by the administration of wild-type SerpinB3 (SerpinB3-WT) or active loop-deleted recombinant SerpinB3 protein (SerpinB3-D) in a rat model of ischemia (60 min)/reperfusion (60 min) (I/R). A time-dependent increase of SerpinB3, both at transcription and protein level, was found in ischemic livers after 60, 120 and 180 min. SerpinB3-WT decreased polymorphonuclear cell infiltration and serum enzymes and increased ATP when compared with I/R group. These events were not obtained using SerpinB3-D. No significant changes in both liver SerpinB3 mRNA and protein were found in all I/R groups considered. The present data show that the administration of SerpinB3-WT reduced the I/R injury and this effect appears to be dependent on its anti-protease activity.
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Ethics Approval
The use and care of animals in this experimental study was approved by the Italian Ministry of Health and by the University Commission for Animal Care: Protocol N° 533/2020 and Protocol N° 274/2021How to Cite
Turato, C., Vairetti, M., Cagna, M., Biasiolo, A., Ferrigno, A., Quarta, S., … Di Pasqua, L. G. (2022). SerpinB3 administration protects liver against ischemia-reperfusion injury. European Journal of Histochemistry, 66(4). https://doi.org/10.4081/ejh.2022.3561
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