https://doi.org/10.4081/ejh.2023.3653

Knockdown of ARHGAP30 inhibits ovarian cancer cell proliferation, migration, and invasiveness by suppressing the PI3K/AKT/mTOR signaling pathway

Vol. 67 No. 2 (2023)
Submitted: 21 January 2023
Accepted: 22 April 2023
Published: 11 May 2023
ARHGAP30, ovarian cancer, cell invasiveness, PI3K/AKT/mTOR pathway
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Authors

Xiaoyan Chu
https://orcid.org/0009-0000-7258-6176
College of Medicine, Nanchang University, Nanchang; Department of Gynecological Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi, China.
Jun Lou
Department of Gynecological Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi, China.
Yun Yi
Department of Gynecological Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi, China.
Linlin Zhong
Department of Gynecological Oncology, Jiangxi Cancer Hospital, Nanchang, Jiangxi, China.
Ouping Huang
xiaoli0775@126.com
College of Medicine, Nanchang University, Nanchang; Key Laboratory of Women’s Reproductive Health of Jiangxi Province, Jiangxi Provincial Maternal and Child Health Hospital, Nanchang, Jiangxi, China.

The mortality and morbidity rates of ovarian cancer (OC) are high, but the underlying mechanisms of OC have not been characterized. In this study, we determined the role of Rho GTPase Activating Protein 30 (ARHGAP30) in OC progression. We measured ARHGAP30 abundance in OC tissue samples and cells using immunohistochemistry (IHC) and RT-qPCR. EdU, transwell, and annexin V/PI apoptosis assays were used to evaluate proliferation, invasiveness, and apoptosis of OC cells, respectively. The results showed that ARHGAP30 was overexpressed in OC tissue samples and cells. Inhibition of ARHGAP30 suppressed growth and metastasis of OC cells, and enhanced apoptosis. Knockdown of ARHGAP30 in OC cells significantly inhibited the PI3K/AKT/mTOR pathway. Treatment with the PI3K/AKT/mTOR pathway inhibitor buparlisib simulated the effects of ARHGAP30 knockdown on growth, invasiveness, and apoptosis of OC cells. Following buparlisib treatment, the expression levels of p-PI3K, p-AKT, and p-mTOR were significantly decreased. Furthermore, buparlisib inhibited the effects of ARHGAP30 upregulation on OC cell growth and invasiveness. In conclusion, ARHGAP30 regulated the PI3K/AKT/mTOR pathway to promote progression of OC.

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Ethics Approval

The current study was approved by the Medical Ethics Committee of Jiangxi Cancer Hospital (no. 2022.023, date 2022.10.10)

How to Cite

Chu, X., Lou, J., Yi, Y., Zhong, L., & Huang, O. (2023). Knockdown of ARHGAP30 inhibits ovarian cancer cell proliferation, migration, and invasiveness by suppressing the PI3K/AKT/mTOR signaling pathway. European Journal of Histochemistry, 67(2). https://doi.org/10.4081/ejh.2023.3653
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