https://doi.org/10.4081/ejh.2023.3679
The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration
Submitted: 9 February 2023
Accepted: 17 February 2023
Published: 23 February 2023
Accepted: 17 February 2023
Abstract Views: 916
PDF: 545
HTML: 22
HTML: 22
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Hydrogels based on various polymeric materials have been successfully developed in recent years for a variety of skin applications. Several studies have shown that hydrogels with regenerative, antibacterial, and antiinflammatory properties can provide faster and better healing outcomes, particularly in chronic diseases where the normal physiological healing process is significantly hampered. Various experimental tests are typically performed to assess these materials' ability to promote angiogenesis, re-epithelialization, and the production and maturation of new extracellular matrix. Immunohistochemistry is important in this context because it allows for the visualization of in situ target tissue factors involved in the various stages of wound healing using antibodies labelled with specific markers detectable with different microscopy techniques. This review provides an overview of the various immunohistochemical techniques that have been used in recent years to investigate the efficacy of various types of hydrogels in assisting skin healing processes. The large number of scientific articles published demonstrates immunohistochemistry's significant contribution to the development of engineered biomaterials suitable for treating skin injuries.
Wong R, Geyer S, Weninger W, Guimberteau JC, Wong JK. The dynamic anatomy and patterning of skin. Exp Dermatol 2016;25:92-8.
DOI: https://doi.org/10.1111/exd.12832
Dąbrowska AK, Spano F, Derler S, Adlhart C, Spencer ND, Rossi RM. The relationship between skin function, barrier properties, and body-dependent factors. Skin Res Technol 2018;24:165-74.
DOI: https://doi.org/10.1111/srt.12424
Lee H-J, Kim M. Skin barrier function and the microbiome. Int J Mol Sci 2022;23:13071.
DOI: https://doi.org/10.3390/ijms232113071
Santema TB, Poyck PPC, Ubbink DT. Skin grafting and tissue replacement for treating foot ulcers in people with diabetes. Cochrane Database Syst Rev 2016;2:CD011255.
DOI: https://doi.org/10.1002/14651858.CD011255.pub2
Tawfeek HM, Abou-Taleb DAE, Badary DM, Ibrahim M, Abdellatif AAH. Pharmaceutical, clinical, and immunohistochemical studies of metformin hydrochloride topical hydrogel for wound healing application. Arch Dermatol Res 2020;312:113-21.
DOI: https://doi.org/10.1007/s00403-019-01982-1
Varkey M, Ding J, Tredget EE. Advances in skin substitutes - Potential of tissue engineered skin for facilitating anti-fibrotic healing. J Funct Biomater 2015;6:547-63.
DOI: https://doi.org/10.3390/jfb6030547
Kamoun EA, Kenawy ERS, Chen X. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. J Adv Res 2017;8:217-33.
DOI: https://doi.org/10.1016/j.jare.2017.01.005
Carton F, Malatesta M. In vitro models of biological barriers for nanomedical research. Int J Mol Sci 2022;23:8910.
DOI: https://doi.org/10.3390/ijms23168910
Carton F, Malatesta M. Assessing the interactions between nanoparticles and biological barriers in vitro: A new challenge for microscopy techniques in nanomedicine. Eur J Histochem 2022;66:3603.
DOI: https://doi.org/10.4081/ejh.2022.3603
Tan SH, Chua DAC, Tang JRJ, Bonnard C, Leavesley D, Liang K. Design of hydrogel-based scaffolds for in vitro three-dimensional human skin model reconstruction. Acta Biomater 2022;153:13-37.
DOI: https://doi.org/10.1016/j.actbio.2022.09.068
Hao R, Cui Z, Zhang X, Tian M, Zhang L, Rao F, et al. Rational design and preparation of functional hydrogels for skin wound healing. Front Chem 2022;9:839055.
DOI: https://doi.org/10.3389/fchem.2021.839055
Zheng W, Wang J, Xie L, Xie H, Chen C, Zhang C, et al. An injectable thermosensitive hydrogel for sustained release of apelin-13 to enhance flap survival in rat random skin flap. J Mater Sci Mater Med 2019;30:106.
DOI: https://doi.org/10.1007/s10856-019-6306-y
Zagórska-Dziok M, Sobczak M. Hydrogel-based active substance release systems for cosmetology and dermatology application: A review. Pharmaceutics 2020;12:396.
DOI: https://doi.org/10.3390/pharmaceutics12050396
Fu W, Xu P, Feng B, Lu Y, Bai J, Zhang J, et al. A hydrogel derived from acellular blood vessel extracellular matrix to promote angiogenesis. J Biomater Appl 2019;33:1301-13.
DOI: https://doi.org/10.1177/0885328219831055
Cui L, Li J, Guan S, Zhang K, Zhang K, Li J. Injectable multifunctional CMC/HA-DA hydrogel for repairing skin injury. Mater Today Bio 2022;14:100257.
DOI: https://doi.org/10.1016/j.mtbio.2022.100257
Abou El-Ezz D, Abdel-Rahman LH, Al-Farhan BS, Mostafa DA, Ayad EG, Basha MT, et al. Enhanced in vivo wound healing efficacy of a novel hydrogel loaded with copper (II) Schiff base quinoline complex (CuSQ) solid lipid nanoparticles. Pharmaceuticals 2022;15:978.
DOI: https://doi.org/10.3390/ph15080978
Lacatusu I, Istrati D, Bordei N, Popescu M, Seciu AM, Panteli LM, et al. Synergism of plant extract and vegetable oils-based lipid nanocarriers: Emerging trends in development of advanced cosmetic prototype products. Mater Sci Eng C 2020;108:110412.
DOI: https://doi.org/10.1016/j.msec.2019.110412
Kamar SS, Abdel-Kader DH, Rashed LA. Beneficial effect of curcumin nanoparticles-hydrogel on excisional skin wound healing in type-I diabetic rat: Histological and immunohistochemical studies. Ann Anat 2019;222:94-102.
DOI: https://doi.org/10.1016/j.aanat.2018.11.005
Zhu Q, Jiang M, Liu Q, Yan S, Feng L, Lan Y, et al. Enhanced healing activity of burn wound infection by a dextran-HA hydrogel enriched with sanguinarine. Biomater Sci 2018;6:2472-86.
DOI: https://doi.org/10.1039/C8BM00478A
Mohamad N, Loh EYX, Fauzi MB, Ng MH, Mohd Amin MCI. In vivo evaluation of bacterial cellulose/acrylic acid wound dressing hydrogel containing keratinocytes and fibroblasts for burn wounds. Drug Deliv Transl Res 2019;9:444-52.
DOI: https://doi.org/10.1007/s13346-017-0475-3
Loh EYX, Mohamad N, Fauzi MB, Ng MH, Ng SF, Mohd Amin MCI. Development of a bacterial cellulose-based hydrogel cell carrier containing keratinocytes and fibroblasts for full-thickness wound healing. Sci Rep 2018;8:2875.
DOI: https://doi.org/10.1038/s41598-018-21174-7
Fan F, Saha S, Hanjaya-Putra D. Biomimetic hydrogels to promote wound healing. Front Bioeng Biotechnol 2021;9:718377.
DOI: https://doi.org/10.3389/fbioe.2021.718377
Subramaniam T, Shaiful Hadi N, Sulaiman S, Fauzi MB, Hj Idrus RB, Chowdhury SR, et al. Comparison of three different skin substitutes in promoting wound healing in an ovine model. Burns 2022;48:1198-208.
DOI: https://doi.org/10.1016/j.burns.2021.08.012
Gad SB, Hafez MH, El-Sayed YS. Platelet-rich plasma and/or sildenafil topical applications accelerate and better repair wound healing in rats through regulation of proinflammatory cytokines and collagen/TGF-β1 pathway. Environ Sci Pollut Res Int 2020;27:40757-68.
DOI: https://doi.org/10.1007/s11356-020-10042-5
Chen X, Cao X, Jiang H, Che X, Xu X, Ma B, et al. SIKVAV-modified chitosan hydrogel as a skin substitutes for wound closure in mice. Molecules 2018;23:2611.
DOI: https://doi.org/10.3390/molecules23102611
Napimoga MH, Clemente-Napimoga JT, Machabanski NM, Juliani MEA, Acras PHBC, Macedo CG, et al. The 15d‑PGJ2 hydrogel ameliorates atopic dermatitis through suppression of the immune response. Mol Med Rep 2019;19:4536-44.
DOI: https://doi.org/10.3892/mmr.2019.10156
Kang X, Lei J, Yang C, Zhang P, Li X, Zheng S, et al. A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring. Biomater Sci 2022;10:6024-36.
DOI: https://doi.org/10.1039/D2BM00935H
Takeuchi T, Ito M, Yamaguchi S, Watanabe S, Honda M, Imahashi T, et al. Hydrocolloid dressing improves wound healing by increasing M2 macrophage polarization in mice with diabetes. Nagoya J Med Sci 2020;82:487-98.
Teng Y, Li S, Tang H, Tao X, Fan Y, Huang Y. Medical applications of hydrogels in skin infections: A review. Infect Drug Resist 2023;16:391-401.
DOI: https://doi.org/10.2147/IDR.S396990
Fernandez-Yague MA, Hymel LA, Olingy CE, McClain C, Ogle ME, García JR, et al. Analyzing immune response to engineered hydrogels by hierarchical clustering of inflammatory cell subsets. Sci Adv 2022;8:eabd8056.
DOI: https://doi.org/10.1126/sciadv.abd8056
Huang Y, Yang N, Teng D, Mao R, Hao Y, Ma X, et al. Antibacterial peptide NZ2114-loaded hydrogel accelerates Staphylococcus aureus-infected wound healing. Appl Microbiol Biotechnol 2022;106:3639-56.
DOI: https://doi.org/10.1007/s00253-022-11943-w
Chen Z, Wang L, Guo C, Qiu M, Cheng L, Chen K, et al. Vascularized polypeptide hydrogel modulates macrophage polarization for wound healing. Acta Biomaterialia 2023;155:218-34.
DOI: https://doi.org/10.1016/j.actbio.2022.11.002
Neves LMG, Parizotto NA, Tim CR, Floriano EM, Lopez RFV, Venâncio T, et al. Polysaccharide-rich hydrogel formulation combined with photobiomodulation repairs UV-induced photodamage in mice skin. Wound Repair Regen 2020;28:645-55.
DOI: https://doi.org/10.1111/wrr.12826
Wallace HA, Basehore BM, Zito PM. Wound healing phases. In: StatPearls [Internet]. Treasure Island: StatPearls Publishing; 2022.
Muire PJ, Thompson MA, Christy RJ, Natesan S. Advances in immunomodulation and immune engineering approaches to improve healing of extremity wounds. Int J Mol Sci 2022;23:4074.
DOI: https://doi.org/10.3390/ijms23084074
Cappellozza E, Boschi F, Sguizzato M, Esposito E, Cortesi R, Malatesta M, et al. A spectrofluorometric analysis to evaluate transcutaneous biodistribution of fluorescent nanoparticulate gel formulations. Eur J Histochem 2022;66:3321.
DOI: https://doi.org/10.4081/ejh.2022.3321
Martínez-Cuazitl A, Gómez-García MDC, Hidalgo-Alegria O, Flores OM, Núñez-Gastélum JA, Martínez ESM, et al. Characterization of polyphenolic compounds from bacopa procumbens and their effects on wound-healing process. Molecules 2022;27:6521.
DOI: https://doi.org/10.3390/molecules27196521
Remmele W, Stegner HE. [Recommendation for uniform definition of an immunoreactive score (IRS) for immunohistochemical estrogen receptor detection (ER-ICA) in breast cancer tissue].[Article in German]. Pathologe 1987;8:138-40.
How to Cite
Carton, F. (2023). The contribution of immunohistochemistry to the development of hydrogels for skin repair and regeneration. European Journal of Histochemistry, 67(1). https://doi.org/10.4081/ejh.2023.3679
Copyright (c) 2023 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Giulia Ronchi, Pierluigi Tos, Elia Angelino, Luisa Muratori, Simone Reano, Nicoletta Filigheddu, Andrea Graziani, Stefano Geuna, Stefania Raimondo, Effect of unacylated ghrelin on peripheral nerve regeneration , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
- Andrea Amaroli, Sara Ferrando, Marina Pozzolini, Lorenzo Gallus, Steven Parker, Stefano Benedicenti, The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- E. Fantinato, L. Milani, G. Sironi, Sox9 expression in canine epithelial skin tumors , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- S. Huang, S. Guo, F. Guo, Q. Yang, X. Xiao, M. Murata, S. Ohnishi, S. Kawanishi, N. Ma, CD44v6 expression in human skin keratinocytes as a possible mechanism for carcinogenesis associated with chronic arsenic exposure , European Journal of Histochemistry: Vol. 57 No. 1 (2013)
- Sara Salucci, Sabrina Burattini, Francesca Buontempo, Alberto Maria Martelli, Elisabetta Falcieri, Michela Battistelli, Protective effect of different antioxidant agents in UVB-irradiated keratinocytes , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
- Mariko Kawai, Yu-Ki Ohmori, Mai Nishino, Masayo Yoshida, Kaori Tabata, Do-Saku Hirota, Ayako Ryu-Mon, Hiromitsu Yamamoto, Junya Sonobe, Yo-Hei Kataoka, Noriko Shiotsu, Mika Ikegame, Hiroki Maruyama, Toshio Yamamoto, Kazuhisa Bessho, Kiyoshi Ohura, Determination of cell fate in skeletal muscle following BMP gene transfer by in vivo electroporation , European Journal of Histochemistry: Vol. 61 No. 2 (2017)
- Manxiu Cao, Lei Zhang, Jiaqi Chen, Cangyu Wang, Junhong Zhao, Xiang Liu, Yongjing Yan, Yue Tang, Zixiu Chen, Haihong Li, Differential antigen expression between human apocrine sweat glands and eccrine sweat glands , European Journal of Histochemistry: Vol. 67 No. 1 (2023)
- You-Jie Liu, Hua-Jun Wang, Zhao-Wen Xue, Lek-Hang Cheang, Man-Seng Tam, Ri-Wang Li, Jie-Ruo Li, Hui-Ge Hou, Xiao-Fei Zheng, Long noncoding RNA H19 accelerates tenogenic differentiation by modulating miR-140-5p/VEGFA signaling , European Journal of Histochemistry: Vol. 65 No. 3 (2021)
- E. Ayarza, M. González, F. López, R. Fernández-Donoso, J. Page, S. Berrios, Alterations in chromosomal synapses and DNA repair in apoptotic spermatocytes of Mus m. domesticus , European Journal of Histochemistry: Vol. 60 No. 2 (2016)
- J. Hansson, A.E. Ericsson, H. Axelson, M.E. Johansson, Species diversity regarding the presence of proximal tubular progenitor cells of the kidney , European Journal of Histochemistry: Vol. 60 No. 1 (2016)
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
