https://doi.org/10.4081/ejh.2023.3688
Clinicopathological assessment of PD-1/PD-L1 immune checkpoint expression in desmoid tumors
Submitted: 21 February 2023
Accepted: 17 April 2023
Published: 26 April 2023
Accepted: 17 April 2023
Abstract Views: 420
PDF: 328
HTML: 15
HTML: 15
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
The details of immune molecules' expression in desmoid tumors (DTs) remain unclear. This study aimed to determine the expression status of the programmed death-1/programmed death ligand 1 (PD1/PD-L1) immune checkpoint mechanism in DTs. The study included patients with DTs (n=9) treated at our institution between April 2006 and December 2012. Immunostaining for CD4, CD8, PD-1, PD-L1, interleukin-2 (IL-2), and interferon-gamma (IFN-γ) was performed on pathological specimens harvested during the biopsy. The positivity rate of each immune component was calculated as the number of positive cells/total cells. The positivity rate was quantified and correlations between the positivity rates of each immune molecule were also investigated. Immune molecules other than PD-1 were stained in tumor cells and intra-tumor infiltrating lymphocytes. The mean ± SD expression rates of β-catenin, CD4, CD8, PD-1, PD-L1, IL-2, and IFN-ɤ were 43.9±18.9, 14.6±6.80, 0.75±4.70, 0±0, 5.1±6.73, 8.75±6.38, and 7.03±12.1, respectively. The correlation between β-catenin and CD4 was positively moderate (r=0.49); β-catenin and PD-L1, positively weak (r=0.25); CD4 and PD-L1, positively medium (r=0.36); CD8 and IL-2, positively medium (r=0.38); CD8 and IFN-ɤ, positively weak (r=0.28); and IL-2 and IFN-ɤ, positively medium (r=0.36). Our findings suggest that PD-L1-centered immune checkpoint mechanisms may be involved in the tumor microenvironment of DTs.
Tsukamoto S, Takahama T, Mavrogenis AF, Tanaka Y, Tanaka Y, Errani C. Clinical outcomes of medical treatments for progressive desmoid tumors following active surveillance: a systematic review. Musculoskelet Surg 2023;107:7-18.
DOI: https://doi.org/10.1007/s12306-022-00738-x
Prete F, Rotelli M, Stella A, Calculli G, Sgaramella LI, Amati A, et al. Intraabdominal sporadic desmoid tumors and inflammation: an updated literature review and presentation and insights on pathogenesis of synchronous sporadic mesenteric desmoid tumors occurring after surgery for necrotizing pancreatitis. Clin Exp Med 2022. Online Ahead of Print.
DOI: https://doi.org/10.1007/s10238-022-00849-6
Bansal A, Goyal S, Goyal A, Jana M. WHO classification of soft tissue tumours 2020: an update and simplified approach for radiologists. Eur J Radiol 2021;143:109937.
DOI: https://doi.org/10.1016/j.ejrad.2021.109937
Raveendranadh A, Goutham M, Gowda C, Hegde K, Monappa V, Rodrigues G. Anterior abdominal wall metastasis following curative resection and chemoradiation of rectal cancer masquerading as a desmoid tumour: a clinical conundrum. J Taibah Univ Med Sci 2022;17:146-9.
DOI: https://doi.org/10.1016/j.jtumed.2021.09.001
Siozopoulou V, Marcq E, Jacobs J, Zwaenepoel K, Hermans C, Brauns J, et al. Desmoid tumors display a strong immune infiltration at the tumor margins and no PD-L1-driven immune suppression. Cancer Immunol Immunother 2019;68:1573-83.
DOI: https://doi.org/10.1007/s00262-019-02390-0
Zhao J, Cheng F, Yao Z, Zheng B, Niu Z, He W. Surgical management of a giant desmoid fibromatosis of abdominal wall with vessels invasion in a young man: a case report and review of the literature. Front Surg 2022;9:851164.
DOI: https://doi.org/10.3389/fsurg.2022.851164
Lazar AJF, Tuvin D, Hajibashi S, Habeeb S, Bolshakov S, Mayordomo-Aranda E, et al. Specific mutations in the beta-catenin gene (CTNNB1) correlate with local recurrence in sporadic desmoid tumors. Am J Pathol 2008;173:1518-27.
DOI: https://doi.org/10.2353/ajpath.2008.080475
Sanchez-Mete L, Ferraresi V, Caterino M, Martayan A, Terrenato I, Mannisi E, et al. Desmoid tumors characteristics, clinical management, active surveillance, and description of our FAP case series. J Clin Med 2020;9:4012.
DOI: https://doi.org/10.3390/jcm9124012
Aelvoet AS, Struik D, Bastiaansen BAJ, Bemelman WA, Hompes R, Bossuyt PMM, et al. Colectomy and desmoid tumours in familial adenomatous polyposis: a systematic review and meta-analysis. Fam Cancer 2022;21:429-39.
DOI: https://doi.org/10.1007/s10689-022-00288-y
Escobar C, Munker R, Thomas JO, Li BD, Burton GV. Update on desmoid tumors. Ann Oncol 2012;23:562-9.
DOI: https://doi.org/10.1093/annonc/mdr386
Al-Jazrawe M, Au M, Alman B. Optimal therapy for desmoid tumors: current options and challenges for the future. Expert Rev Anticancer Ther 2015;15:1443-58.
DOI: https://doi.org/10.1586/14737140.2015.1096203
Tsukamoto S, Tanzi P, Mavrogenis AF, Akahane M, Kido A, Tanaka Y, et al. Upfront surgery is not advantageous compared to more conservative treatments such as observation or medical treatment for patients with desmoid tumors. BMC Musculoskelet Disord 2021;22:12.
DOI: https://doi.org/10.1186/s12891-020-03897-9
Dong H, Strome SE, Salomao DR, Tamura H, Hirano F, Flies DB, et al. Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion. Nat Med 2002;8:793-800.
DOI: https://doi.org/10.1038/nm730
Ghebeh H, Mohammed S, Al-Omair A, Qattan A, Lehe C, Al-Qudaihi G, et al. The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia 2006;8:190-8.
DOI: https://doi.org/10.1593/neo.05733
Mahoney KM, Freeman GJ, McDermott DF. The next immune-checkpoint inhibitors: PD-1/PD-L1 blockade in melanoma. Clin Ther 2015;37:764-82.
DOI: https://doi.org/10.1016/j.clinthera.2015.02.018
Gong J, Chehrazi-Raffle A, Reddi S, Salgia R. Development of PD-1 and PD-L1 inhibitors as a form of cancer immunotherapy: a comprehensive review of registration trials and future considerations. J Immunother Cancer 2018;6:8.
DOI: https://doi.org/10.1186/s40425-018-0316-z
Hashimoto K, Nishimura S, Ito T, Akagi M. Characterization of PD-1/PD-L1 immune checkpoint expression in soft tissue sarcomas. Eur J Histochem 2021;65:3203.
DOI: https://doi.org/10.4081/ejh.2021.3203
Colombo C, Belfiore A, Paielli N, De Cecco L, Canevari S, Laurini E, et al. β-Catenin in desmoid-type fibromatosis: deep insights into the role of T41A and S45F mutations on protein structure and gene expression. Mol Oncol 2017;11:1495-507.
DOI: https://doi.org/10.1002/1878-0261.12101
Kaewkangsadan V, Verma C, Eremin JM, Cowley G, Ilyas M, Eremin O. Crucial contributions by T lymphocytes (effector, regulatory, and checkpoint inhibitor) and cytokines (TH1, TH2, and TH17) to a pathological complete response induced by neoadjuvant chemotherapy in women with breast cancer. J Immunol Res 2016;2016:4757405.
DOI: https://doi.org/10.1155/2016/4757405
Kakavand H, Wilmott JS, Long GV, Scolyer RA. Targeted therapies and immune checkpoint inhibitors in the treatment of metastatic melanoma patients: a guide and update for pathologists. Pathology 2016;48:194-202.
DOI: https://doi.org/10.1016/j.pathol.2015.12.010
Petitprez F, de Reyniès A, Keung EZ, Chen TW, Sun CM, Calderaro J, et al. B cells are associated with survival and immunotherapy response in sarcoma. Nature 2020;577:556-60.
DOI: https://doi.org/10.1038/s41586-019-1906-8
Hu-Lieskovan S, Ribas A. New combination strategies using programmed cell death 1/programmed cell death ligand 1 checkpoint inhibitors as a backbone. Cancer J 2017;23:10-22.
DOI: https://doi.org/10.1097/PPO.0000000000000246
Tejpar S, Nollet F, Li C, Wunder JS, Michils G, dal Cin P, et al. Predominance of beta-catenin mutations and beta-catenin dysregulation in sporadic aggressive fibromatosis (desmoid tumor). Oncogene 1999;18:6615-20.
DOI: https://doi.org/10.1038/sj.onc.1203041
Gebert C, Hardes J, Kersting C, August C, Supper H, Winkelmann W, et al. Expression of beta-catenin and p53 are prognostic factors in deep aggressive fibromatosis. Histopathology 2007;50:491-7.
DOI: https://doi.org/10.1111/j.1365-2559.2007.02619.x
Ethics Approval
The current study was approved by the Kindai University Ethics Committee (N. 31-187; Date 16 January 2020)How to Cite
Hashimoto, K., Nishimura, S., Shinyashiki, Y., Ito, T., Kakinoki, R., & Akagi, M. (2023). Clinicopathological assessment of PD-1/PD-L1 immune checkpoint expression in desmoid tumors. European Journal of Histochemistry, 67(2). https://doi.org/10.4081/ejh.2023.3688
Copyright (c) 2023 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- L. Pilloni, P. Bianco, E. DiFelice, S. Cabras, M.E. Castellanos, L. Atzori, C. Ferreli, P. Mulas, S. Nemolato, G. Faa, The usefulness of c-Kit in the immunohistochemical assessment of melanocytic lesions , European Journal of Histochemistry: Vol. 55 No. 2 (2011)
- A. Makowiecka, A. Simiczyjew, D. Nowak, A.J. Mazur, Varying effects of EGF, HGF and TGFβ on formation of invadopodia and invasiveness of melanoma cell lines of different origin , European Journal of Histochemistry: Vol. 60 No. 4 (2016)
- G. Tsirakis, C. A. Pappa, M. Kaparou, V. Katsomitrou, A. Hatzivasili, T. Alegakis, A. Xekalou, E. N. Stathopoulos, M. Alexandrakis, Assessment of proliferating cell nuclear antigen and its relationship with proinflammatory cytokines and parameters of disease activity in multiple myeloma patients , European Journal of Histochemistry: Vol. 55 No. 3 (2011)
- Francesca Diomede, Maria Zingariello, Marcos F.X.B. Cavalcanti, Ilaria Merciaro, Jacopo Pizzicannella, Natalia de Isla, Sergio Caputi, Patrizia Ballerini, Oriana Trubiani, MyD88/ERK/NFkB pathways and pro-inflammatory cytokines release in periodontal ligament stem cells stimulated by Porphyromonas gingivalis , European Journal of Histochemistry: Vol. 61 No. 2 (2017)
- Kazuhiko Hashimoto, Shunji Nishimura, Tomohiko Ito, Masao Akagi, Characterization of PD-1/PD-L1 immune checkpoint expression in soft tissue sarcomas , European Journal of Histochemistry: Vol. 65 No. 3 (2021)
- Yan Yan Li, Ya Ping Feng, Li Liu, Jin Ke, Xing Long, Inhibition of HMGB1 suppresses inflammation and catabolism in temporomandibular joint osteoarthritis via NF-κB signaling pathway , European Journal of Histochemistry: Vol. 66 No. 3 (2022)
- G. Chene, N. Radosevic-Robin, A.S. Tardieu, A. Cayre, I. Raoelfils, P. Dechelotte, J. Dauplat, F. Penault Llorca, Morphological and immunohistochemical study of ovarian and tubal dysplasia associated with tamoxifen , European Journal of Histochemistry: Vol. 58 No. 2 (2014)
- G. Latella, A. Vetuschi, R. Sferra, S. Speca, E. Gaudio, Localization of avβ6 integrin- TGF-β1/Smad3, mTOR and PPARgg in experimental colorectal fibrosis , European Journal of Histochemistry: Vol. 57 No. 4 (2013)
- Simone Carotti, Giuseppe Perrone, Michelina Amato, Umberto Vespasiani Gentilucci, Daniela Righi, Maria Francesconi, Claudio Pellegrini, Francesca Zalfa, Maria Zingariello, Antonio Picardi, Andrea Onetti Muda, Sergio Morini, Reelin expression in human liver of patients with chronic hepatitis C infection , European Journal of Histochemistry: Vol. 61 No. 1 (2017)
- Liqin Xi, Chen Wang, Pengyu Chen, Qi Yang, Ruiqi Hu, Haolin Zhang, Qiang Weng, Meiyu Xu, Expressions of IL-6, TNF-α and NF-κB in the skin of Chinese brown frog (Rana dybowskii) , European Journal of Histochemistry: Vol. 61 No. 4 (2017)
You may also start an advanced similarity search for this article.
