https://doi.org/10.4081/ejh.2023.3742

Pretreatment with geniposide mitigates myocardial ischemia/reperfusion injury by modulating inflammatory response through TLR4/NF-κB pathway

Vol. 67 No. 3 (2023)
Submitted: 31 March 2023
Accepted: 11 August 2023
Published: 8 September 2023
Geniposide, myocardial ischemia/reperfusion injury, nuclear factor-κB (NF-κB), inflammation, TLR4
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Authors

Yanmei Yao
Department of General Medicine, The Affiliated Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Leqing Lin
Department of Critical Care Medicine, The Affiliated Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Wenxue Tang
Department of Critical Care Medicine, The Affiliated Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Yueliang Shen
Department of Pathophysiology, Zhejiang University Medical College, Hangzhou, Zhejiang, China.
Fayu Chen
Department of General Medicine, The Affiliated Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Ning Li
liningrestable@163.com
Department of Hematology and Oncology, The Affiliated Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Baiyong Wang
Department of Critical Care Medicine, The Affiliated Hospital, Hangzhou Normal University, Hangzhou, Zhejiang, China.

Geniposide (GEN), a medical herb, is known for its therapeutic applications in cardiovascular diseases, though its efficacy in treating myocardial ischemia/reperfusion injury (MI/RI) is yet to be fully elucidated. This study is an endeavor to explore the potential protective mechanism of GEN against MI/RI. To simulate the MI/RI condition, the left anterior descending artery was occluded for 30 min, followed by a reperfusion period of 120 min in a rat model. Three dosages (50, 100, or 150 mg/kg) of GEN were intraperitoneally injected to the Sprague-Dawley rats once a day, for seven days before the ligation of the artery. The rats were categorized into sham group, MI/RI group, and three different dosages GEN-treated groups. As the results showed, the pretreatment with GEN mitigated myocardial injury, reduced infarct volume, inhibited apoptosis, enhanced superoxide dismutase activity, and decreased malondialdehyde and myeloperoxidase activity, as well as serum creatine kinase-MB and lactate dehydrogenase levels. Moreover, GEN ameliorated MI/RI by downregulating protein expression of toll-like receptor 4, myeloid differentiation primary response 88, and p-nuclear factor-κB. In conclusion, the pretreatment of GEN may be considered as a potential therapeutic option for MI/RI.

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Ethics Approval

The Hangzhou Eyong Biotechnological Co., Ltd. Animal Experiment Center approved and authorized the animal experiments

Supporting Agencies

Key Medical Disciplines of Hangzhou , Hangzhou Special Project for Development and Support of Bio-pharmaceutical and Health Industries, Health and Pharmaceutical Science and Technology Project of Zhejiang Province

How to Cite

Yao, Y., Lin, L., Tang, W., Shen, Y., Chen, F., Li, N., & Wang, B. (2023). Pretreatment with geniposide mitigates myocardial ischemia/reperfusion injury by modulating inflammatory response through TLR4/NF-κB pathway. European Journal of Histochemistry, 67(3). https://doi.org/10.4081/ejh.2023.3742
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