Expression and localization of &#945;<sub>2A</sub>-adrenergic receptor in the rat post-natal developing cochlea

Chaoyong Tian; Yang Yang; Yao Li; Fei Sun; Juan Qu; Dingjun Zha

doi:10.4081/ejh.2023.3748

Authors

Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi’an, Shannxi Province, China.

Yang Yang

Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi’an, Shannxi Province, China.

Yao Li

Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi’an, Shannxi Province, China.

Fei Sun

https://orcid.org/0000-0003-2378-2896

Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi’an, Shannxi Province, China.

Juan Qu

Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi’an, Shannxi Province, China.

Dingjun Zha

zhadjun@fmmu.edu.cn

Department of Otolaryngology Head and Neck Surgery, Xijing Hospital, Air Force Medical University, Xi’an, Shannxi Province, China.

Lots of adrenergic receptors (ARs) are widely present across the auditory pathways and are positioned to affect auditory and vestibular functions. However, noradrenergic regulation in the cochlea has not been well characterized. In this study, a rat model of noise-induced hearing loss was developed to investigate the expression of α_2A-adrenergic receptor (AR) after acoustic trauma, then, we investigated the expression of α_2A-AR in the developing rat cochlea using immunofluorescence, qRT-PCR, and Western blotting. We found that the expression of α_2A-AR significantly increased in rats exposed to noise compared with controls. Immunofluorescence analysis demonstrated that α_2A-AR is localized on hair cells (HCs), spiral ganglion neurons (SGNs), and the stria vascularis (SV) in the postnatal developing cochlea from post-natal day (P) 0 to P28. Furthermore, we observed α_2A-AR mRNA reached a maximum level at P14 and P28 when compared with P0, while no significant differences in α_2A-AR protein levels at the various stages when compared with P0. This study provides direct evidence for the expression of α_2A-AR in HCs, SGNs, and the SV of the cochlea, indicating that norepinephrine might play a vital role in hearing function within the cochlea through α_2A-AR.

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Ethics Approval

All animal procedures were conducted in compliance with the National Research Council's Guide for the Care and Use of Laboratory Animals, and were approved by the Institutional Animal Care and Use Committee of the Air Force Medical University, Xi’an, Shannxi Province, China.

Supporting Agencies

National Natural Science Foundation of China , National Key Research and Development Plan , Natural Science Foundation of Shaanxi , Shaanxi Provincial Clinical Medical Research Center , Research Promotion project from Xijing Hospital

How to Cite

Tian, C., Yang, Y., Li, Y., Sun, F., Qu, J., & Zha, D. (2023). Expression and localization of α<sub>2A</sub>-adrenergic receptor in the rat post-natal developing cochlea. European Journal of Histochemistry, 67(3). https://doi.org/10.4081/ejh.2023.3748

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Expression and localization of α_2A-adrenergic receptor in the rat post-natal developing cochlea