https://doi.org/10.4081/ejh.2023.3908
Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway
Submitted: 7 November 2023
Accepted: 28 November 2023
Published: 12 December 2023
Accepted: 28 November 2023
Abstract Views: 729
PDF: 255
HTML: 3
HTML: 3
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study aimed to explore the role and mechanism of umbilical cord mesenchymal stem cells (UCMSCs) in regulating inflammation of bronchial epithelial cells. Transforming growth factor beta-1 (TGF-β1) was used to induce inflammation in human bronchial epithelial cells. Cell proliferation was detected through CCK8 and cell apoptosis was detected by Annexin V and propidium iodide double staining. E-cadherin and α-smooth muscle actin (α-SMA) were detected by immunofluorescence, and tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in culture medium supernatant were detected by ELISA. The expression of E-cadherin, α-SMA, Sonic hedgehog (Shh), Gli1 and Snail was detected by Western blot analysis. Compared with the control group, bronchial epithelial cells treated with TGF-β1 showed significantly decreased proliferation, increased apoptosis, increased secretion of TNF-α and IL-6, increased expression of α-SMA, Shh, Gli1 and Snail and decreased E-cadherin expression. However, co-culture with UCMSCs inhibited TGF-β1-induced changes in human bronchial epithelial cell proliferation, apoptosis, secretion of TNF-α and IL-6 and activation of the Hedgehog pathway. In conclusion, UCMSCs have protective effects on TGF-β1-induced inflammation in human bronchial epithelial cells by regulating the Hedgehog pathway.
Holgate S, Wenzel S, Postma D, Weiss S, Renz H, Sly P. Asthma. Nature Rev Disease Primers 2015;1:15025.
DOI: https://doi.org/10.1038/nrdp.2015.25
Cohn L, Elias J, Chupp G. Asthma: mechanisms of disease persistence and progression. Ann Rev Immunol 2004;22:789-815.
DOI: https://doi.org/10.1146/annurev.immunol.22.012703.104716
Paim TC, Wink MR. The versatility of mesenchymal stem cells: From regenerative medicine to COVID, what is next? Biocell 2022;46:913-22.
DOI: https://doi.org/10.32604/biocell.2022.018498
Kim O-H, Kim E-R, Park J-H, Lee H-J. Applications of scaffolds: Tools for enhancing the immunomodulation of mesenchymal stromal cells. Biocell 2022;46:1439-43.
DOI: https://doi.org/10.32604/biocell.2022.018921
Tynecka M, Moniuszko M, Eljaszewicz A. Old Friends with unexploited perspectives: current advances in mesenchymal stem cell-based therapies in asthma. Stem Cell Rev Rep 2021;17:1323-42.
DOI: https://doi.org/10.1007/s12015-021-10137-7
Jiang J. Hedgehog signaling mechanism and role in cancer. Semin Cancer Biol 2021;85:107-22.
DOI: https://doi.org/10.1016/j.semcancer.2021.04.003
Xu X, Li Y, Liu G, Li K, Chen P, Gao Y, et al. MiR-378a-3p acts as a tumor suppressor in gastric cancer via directly targeting RAB31 and inhibiting the Hedgehog pathway proteins GLI1/2. Cancer Biol Med 2022;19:1662-82.
DOI: https://doi.org/10.20892/j.issn.2095-3941.2022.0337
Zeng L, Barkat M, Syed S, Shah S, Abbas G, Xu C, et al. Hedgehog signaling: linking embryonic lung development and asthmatic airway remodeling. Cells 2022;11:1774.
DOI: https://doi.org/10.3390/cells11111774
Chen Y, Dong Y, Du X. Lung development: AT1 and AT2 property. Biocell 2020;44:1-5.
DOI: https://doi.org/10.32604/biocell.2020.08041
Ramachandran J, Zhou W, Bardenhagen A, Nasr T, Yates E, Zorn A, et al. Hedgehog regulation of epithelial cell state and morphogenesis in the larynx. eLife 2022;18:e77055.
DOI: https://doi.org/10.7554/eLife.77055.sa2
Choi JY, Hur J, Jeon S, Jung CK, Rhee CK. Effects of human adipose tissue- and bone marrow-derived mesenchymal stem cells on airway inflammation and remodeling in a murine model of chronic asthma. Sci Rep 2022;12:12032.
DOI: https://doi.org/10.1038/s41598-022-16165-8
Bai L, Yu Z, Wang C, Qian G and Wang G. Dual role of TGF-β1 on Fas-induced apoptosis in lung epithelial cells. Respir Physiol Neurobiol 2011;177:241-6.
DOI: https://doi.org/10.1016/j.resp.2011.04.016
Permaul P, Peters MC, Petty CR, Cardet JC, Phipatanakul W. The association of plasma IL-6 with measures of asthma morbidity in a moderate-severe pediatric cohort aged 6-18 years. J Allergy Clin Immunol Pract 2021;9:2916-9.
DOI: https://doi.org/10.1016/j.jaip.2021.02.047
Zhang H, Wang M, Xu Y. Understanding the mechanisms underlying obesity in remodeling the breast tumor immune microenvironment: from the perspective of inflammation. Cancer Biol Med 2023;20:268-86.
DOI: https://doi.org/10.20892/j.issn.2095-3941.2022.0547
Wang C, Cassandras M, Peng T. The role of Hedgehog Signaling in adult lung regeneration and maintenance. J Dev Biol 2019;7:14.
DOI: https://doi.org/10.3390/jdb7030014
Huang L, Jin M, Gu R, Xiao K, Lu M, Huo X, et al. miR-199a-5p Reduces chondrocyte hypertrophy and attenuates osteoarthritis progression via the Indian Hedgehog signal pathway. J Clin Med 2023;12:1313.
DOI: https://doi.org/10.3390/jcm12041313
Liu H, Shi Y, Wan X, Liu Y, Shu H, Huang F, et al. Effect of peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition. Biocell 2022;46:2671-80.
DOI: https://doi.org/10.32604/biocell.2022.018054
Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme JL, Wémeau-Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-β-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol 2012;181:2126-37.
DOI: https://doi.org/10.1016/j.ajpath.2012.08.019
Wang J, Li F, Yang M, Wu J, Zhao J, Gong W, et al. FIZZ1 promotes airway remodeling through the PI3K/Akt signaling pathway in asthma. Exp Therap Med 2014;7:1265-70.
DOI: https://doi.org/10.3892/etm.2014.1580
Wieczfinska J, Pawliczak R. Anti-fibrotic effect of ciglitazone in HRV-induced airway remodelling cell model. J Cell Mol Med 2023;31:133-58.
DOI: https://doi.org/10.1111/jcmm.17790
Y, Kang H, Bang JY, Shin JW, Kim HY, Cho SH, et al. Intratracheal administration of mesenchymal stem cells modulates lung macrophage polarization and exerts anti-asthmatic effects. Sci Rep 2022;12:11728.
DOI: https://doi.org/10.1038/s41598-022-14846-y
Ethics Approval
This study was approved by the Ethics Committee of Fuzhou No.1 Hospital Affiliated with Fujian Medical UniversityHow to Cite
Lin, Q., Yu, T., Li, X., Lin, X., Fan, Y., & Xu, L. (2023). Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway. European Journal of Histochemistry, 67(4). https://doi.org/10.4081/ejh.2023.3908
Copyright (c) 2023 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Andrea Amaroli, Sara Ferrando, Marina Pozzolini, Lorenzo Gallus, Steven Parker, Stefano Benedicenti, The earthworm Dendrobaena veneta (Annelida): A new experimental-organism for photobiomodulation and wound healing , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- Eetmad A. Arafat, Eman M.I. Youssef, Hanaa A. Khalaf, The possible alleviating effect of garlic supplement on the neural retina in a rat model of hypercholesterolemia: a histological and immunohistochemical study , European Journal of Histochemistry: Vol. 65 No. 4 (2021)
- A.M. Schläfli, S. Berezowska, O. Adams, R. Langer, M.P. Tschan, Reliable LC3 and p62 autophagy marker detection in formalin fixed paraffin embedded human tissue by immunohistochemistry , European Journal of Histochemistry: Vol. 59 No. 2 (2015)
- Chaojie He, Yi Yu, Feifan Wang, Wudi Li, Hui Ni, Meixiang Xiang, Pretreatment with interleukin-15 attenuates inflammation and apoptosis by inhibiting NF-κB signaling in sepsis-induced myocardial dysfunction , European Journal of Histochemistry: Vol. 68 No. 2 (2024)
- Lu Liu, Zhankui Liu, Liu Yang, Xue Wu, Jiaying Zhu, Lili Liu, Yang Liu, Lobetyolin suppressed lung cancer in a mouse model by inhibiting epithelial-mesenchymal transition , European Journal of Histochemistry: Vol. 66 No. 3 (2022)
- Sara Salucci, Sabrina Burattini, Francesca Buontempo, Alberto Maria Martelli, Elisabetta Falcieri, Michela Battistelli, Protective effect of different antioxidant agents in UVB-irradiated keratinocytes , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
- Yan Long, Yan Zhao, Xiaoqing Ma, Ya Zeng, Tian Hu, Weijie Wu, Chongtian Deng, Jinyue Hu, Yueming Shen, Endoplasmic reticulum stress contributed to inflammatory bowel disease by activating p38 MAPK pathway , European Journal of Histochemistry: Vol. 66 No. 2 (2022)
- G. Musumeci, C. Loreto, M.L. Carnazza, F. Coppolino, V. Cardile, R. Leonardi, Lubricin is expressed in chondrocytes derived from osteoarthritic cartilage encapsulated in poly(ethylene glycol) diacrylate scaffold , European Journal of Histochemistry: Vol. 55 No. 3 (2011)
- H. Kontro, J.J. Hulmi, P. Rahkila, H. Kainulainen, Cellular and tissue expression of DAPIT, a phylogenetically conserved peptide , European Journal of Histochemistry: Vol. 56 No. 2 (2012)
- E. Akat, H. Arıkan, B. Göçmen, Histochemical and biometric study of the gastrointestinal system of Hyla orientalis (Bedriaga, 1890) (Anura, Hylidae) , European Journal of Histochemistry: Vol. 58 No. 4 (2014)
<< < 15 16 17 18 19 20 21 22 23 24 > >>
You may also start an advanced similarity search for this article.
