https://doi.org/10.4081/ejh.2023.3908
Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway
Submitted: 7 November 2023
Accepted: 28 November 2023
Published: 12 December 2023
Accepted: 28 November 2023
Abstract Views: 729
PDF: 255
HTML: 3
HTML: 3
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This study aimed to explore the role and mechanism of umbilical cord mesenchymal stem cells (UCMSCs) in regulating inflammation of bronchial epithelial cells. Transforming growth factor beta-1 (TGF-β1) was used to induce inflammation in human bronchial epithelial cells. Cell proliferation was detected through CCK8 and cell apoptosis was detected by Annexin V and propidium iodide double staining. E-cadherin and α-smooth muscle actin (α-SMA) were detected by immunofluorescence, and tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in culture medium supernatant were detected by ELISA. The expression of E-cadherin, α-SMA, Sonic hedgehog (Shh), Gli1 and Snail was detected by Western blot analysis. Compared with the control group, bronchial epithelial cells treated with TGF-β1 showed significantly decreased proliferation, increased apoptosis, increased secretion of TNF-α and IL-6, increased expression of α-SMA, Shh, Gli1 and Snail and decreased E-cadherin expression. However, co-culture with UCMSCs inhibited TGF-β1-induced changes in human bronchial epithelial cell proliferation, apoptosis, secretion of TNF-α and IL-6 and activation of the Hedgehog pathway. In conclusion, UCMSCs have protective effects on TGF-β1-induced inflammation in human bronchial epithelial cells by regulating the Hedgehog pathway.
Holgate S, Wenzel S, Postma D, Weiss S, Renz H, Sly P. Asthma. Nature Rev Disease Primers 2015;1:15025.
DOI: https://doi.org/10.1038/nrdp.2015.25
Cohn L, Elias J, Chupp G. Asthma: mechanisms of disease persistence and progression. Ann Rev Immunol 2004;22:789-815.
DOI: https://doi.org/10.1146/annurev.immunol.22.012703.104716
Paim TC, Wink MR. The versatility of mesenchymal stem cells: From regenerative medicine to COVID, what is next? Biocell 2022;46:913-22.
DOI: https://doi.org/10.32604/biocell.2022.018498
Kim O-H, Kim E-R, Park J-H, Lee H-J. Applications of scaffolds: Tools for enhancing the immunomodulation of mesenchymal stromal cells. Biocell 2022;46:1439-43.
DOI: https://doi.org/10.32604/biocell.2022.018921
Tynecka M, Moniuszko M, Eljaszewicz A. Old Friends with unexploited perspectives: current advances in mesenchymal stem cell-based therapies in asthma. Stem Cell Rev Rep 2021;17:1323-42.
DOI: https://doi.org/10.1007/s12015-021-10137-7
Jiang J. Hedgehog signaling mechanism and role in cancer. Semin Cancer Biol 2021;85:107-22.
DOI: https://doi.org/10.1016/j.semcancer.2021.04.003
Xu X, Li Y, Liu G, Li K, Chen P, Gao Y, et al. MiR-378a-3p acts as a tumor suppressor in gastric cancer via directly targeting RAB31 and inhibiting the Hedgehog pathway proteins GLI1/2. Cancer Biol Med 2022;19:1662-82.
DOI: https://doi.org/10.20892/j.issn.2095-3941.2022.0337
Zeng L, Barkat M, Syed S, Shah S, Abbas G, Xu C, et al. Hedgehog signaling: linking embryonic lung development and asthmatic airway remodeling. Cells 2022;11:1774.
DOI: https://doi.org/10.3390/cells11111774
Chen Y, Dong Y, Du X. Lung development: AT1 and AT2 property. Biocell 2020;44:1-5.
DOI: https://doi.org/10.32604/biocell.2020.08041
Ramachandran J, Zhou W, Bardenhagen A, Nasr T, Yates E, Zorn A, et al. Hedgehog regulation of epithelial cell state and morphogenesis in the larynx. eLife 2022;18:e77055.
DOI: https://doi.org/10.7554/eLife.77055.sa2
Choi JY, Hur J, Jeon S, Jung CK, Rhee CK. Effects of human adipose tissue- and bone marrow-derived mesenchymal stem cells on airway inflammation and remodeling in a murine model of chronic asthma. Sci Rep 2022;12:12032.
DOI: https://doi.org/10.1038/s41598-022-16165-8
Bai L, Yu Z, Wang C, Qian G and Wang G. Dual role of TGF-β1 on Fas-induced apoptosis in lung epithelial cells. Respir Physiol Neurobiol 2011;177:241-6.
DOI: https://doi.org/10.1016/j.resp.2011.04.016
Permaul P, Peters MC, Petty CR, Cardet JC, Phipatanakul W. The association of plasma IL-6 with measures of asthma morbidity in a moderate-severe pediatric cohort aged 6-18 years. J Allergy Clin Immunol Pract 2021;9:2916-9.
DOI: https://doi.org/10.1016/j.jaip.2021.02.047
Zhang H, Wang M, Xu Y. Understanding the mechanisms underlying obesity in remodeling the breast tumor immune microenvironment: from the perspective of inflammation. Cancer Biol Med 2023;20:268-86.
DOI: https://doi.org/10.20892/j.issn.2095-3941.2022.0547
Wang C, Cassandras M, Peng T. The role of Hedgehog Signaling in adult lung regeneration and maintenance. J Dev Biol 2019;7:14.
DOI: https://doi.org/10.3390/jdb7030014
Huang L, Jin M, Gu R, Xiao K, Lu M, Huo X, et al. miR-199a-5p Reduces chondrocyte hypertrophy and attenuates osteoarthritis progression via the Indian Hedgehog signal pathway. J Clin Med 2023;12:1313.
DOI: https://doi.org/10.3390/jcm12041313
Liu H, Shi Y, Wan X, Liu Y, Shu H, Huang F, et al. Effect of peroxiredoxin 1 on the biological function of airway epithelial cells and epithelial-mesenchymal transition. Biocell 2022;46:2671-80.
DOI: https://doi.org/10.32604/biocell.2022.018054
Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme JL, Wémeau-Stervinou L, Fabre A, et al. The hedgehog system machinery controls transforming growth factor-β-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis. Am J Pathol 2012;181:2126-37.
DOI: https://doi.org/10.1016/j.ajpath.2012.08.019
Wang J, Li F, Yang M, Wu J, Zhao J, Gong W, et al. FIZZ1 promotes airway remodeling through the PI3K/Akt signaling pathway in asthma. Exp Therap Med 2014;7:1265-70.
DOI: https://doi.org/10.3892/etm.2014.1580
Wieczfinska J, Pawliczak R. Anti-fibrotic effect of ciglitazone in HRV-induced airway remodelling cell model. J Cell Mol Med 2023;31:133-58.
DOI: https://doi.org/10.1111/jcmm.17790
Y, Kang H, Bang JY, Shin JW, Kim HY, Cho SH, et al. Intratracheal administration of mesenchymal stem cells modulates lung macrophage polarization and exerts anti-asthmatic effects. Sci Rep 2022;12:11728.
DOI: https://doi.org/10.1038/s41598-022-14846-y
Ethics Approval
This study was approved by the Ethics Committee of Fuzhou No.1 Hospital Affiliated with Fujian Medical UniversityHow to Cite
Lin, Q., Yu, T., Li, X., Lin, X., Fan, Y., & Xu, L. (2023). Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway. European Journal of Histochemistry, 67(4). https://doi.org/10.4081/ejh.2023.3908
Copyright (c) 2023 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- V. Pibiri, A. Ravarino, C. Gerosa, M.C. Pintus, V. Fanos, G. Faa, Stem/progenitor cells in the developing human cerebellum: an immunohistochemical study , European Journal of Histochemistry: Vol. 60 No. 3 (2016)
- M. Acosta, V. Filippa, F. Mohamed, Folliculostellate cells in pituitary pars distalis of male viscacha: immunohistochemical, morphometric and ultrastructural study , European Journal of Histochemistry: Vol. 54 No. 1 (2010)
- Jindong Li, Jie Kang, Weiyan Liu, Jiazhe Liu, Gaofeng Pan, Anwei Mao, Qing Zhang, Jingfeng Lu, Junbin Ding, Hongchang Li, Docetaxel-resistant triple-negative breast cancer cell-derived exosomal lncRNA LINC00667 reduces the chemosensitivity of breast cancer cells to docetaxel via targeting miR-200b-3p/Bcl-2 axis , European Journal of Histochemistry: Vol. 66 No. 4 (2022)
- H. Zhang, X. Guo, S. Zhong, T. Ge, S. Peng, P. Yu, Z. Zhou, Heterogeneous vesicles in mucous epithelial cells of posterior esophagus of Chinese giant salamander (Andrias davidianus) , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- E. Fantinato, L. Milani, G. Sironi, Sox9 expression in canine epithelial skin tumors , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- Francesca Diomede, Soundara Rajan Thangavelu, Ilaria Merciaro, Monica D'Orazio, Placido Bramanti, Emanuela Mazzon, Oriana Trubiani, Porphyromonas gingivalis lipopolysaccharide stimulation in human periodontal ligament stem cells: role of epigenetic modifications to the inflammation , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
- S. Nemolato, T. Cabras, M.U. Fanari, F. Cau, M. Fraschini, B. Manconi, I. Messana, M. Castagnola, G. Faa, Thymosin beta 4 expression in normal skin, colon mucosa and in tumor infiltrating mast cells , European Journal of Histochemistry: Vol. 54 No. 1 (2010)
- Yan Wang, Jie Sun, Ninghua Yao, Correlation of the AKT/mTOR signaling pathway with the clinicopathological features and prognosis of nasopharyngeal carcinoma , European Journal of Histochemistry: Vol. 65 No. 4 (2021)
- A.J.W. Pinto, I.F.G. de Amorim, L.J. Pinheiro, I.M.V.M. Madeira, C.C. Souza, H. Chiarini-Garcia, M.V. Caliari, W.L. Tafuri, Glycol methacrylate embedding for the histochemical study of the gastrointestinal tract of dogs naturally infected with Leishmania infantum , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- Gianluca Accogli, Giovanni Scillitani, Donatella Mentino, Salvatore Desantis, Characterization of the skin mucus in the common octopus Octopus vulgaris (Cuvier) reared paralarvae , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
<< < 1 2 3 4 5 6 7 8 9 10 > >>
You may also start an advanced similarity search for this article.
