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Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway

Authors

Qiong Lin
Department of Respiratory Medicine, Fuzhou No.1 Hospital Affiliated with Fujian Medical University, Fuzhou, Fujian, China.
Tianxing Yu
Department of Respiratory Medicine, Fuzhou No.1 Hospital Affiliated with Fujian Medical University, Fuzhou, Fujian, China.
Xiaohua Li
Department of Respiratory Medicine, Fuzhou No.1 Hospital Affiliated with Fujian Medical University, Fuzhou, Fujian, China.
Xin Lin
Department of Respiratory Medicine, Fuzhou No.1 Hospital Affiliated with Fujian Medical University, Fuzhou, Fujian, China.
Yong Fan
Cent Lab, Fuzhou No.1 Hospital Affiliated with Fujian Medical University, Fuzhou, Fujian, China.
Liyu Xu
linqiong198911@163.com
Department of Respiratory Medicine, Fuzhou No.1 Hospital Affiliated with Fujian Medical University, Fuzhou, Fujian, China.

This study aimed to explore the role and mechanism of umbilical cord mesenchymal stem cells (UCMSCs) in regulating inflammation of bronchial epithelial cells. Transforming growth factor beta-1 (TGF-β1) was used to induce inflammation in human bronchial epithelial cells. Cell proliferation was detected through CCK8 and cell apoptosis was detected by Annexin V and propidium iodide double staining. E-cadherin and α-smooth muscle actin (α-SMA) were detected by immunofluorescence, and tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) in culture medium supernatant were detected by ELISA. The expression of E-cadherin, α-SMA, Sonic hedgehog (Shh), Gli1 and Snail was detected by Western blot analysis. Compared with the control group, bronchial epithelial cells treated with TGF-β1 showed significantly decreased proliferation, increased apoptosis, increased secretion of TNF-α and IL-6, increased expression of α-SMA, Shh, Gli1 and Snail and decreased E-cadherin expression. However, co-culture with UCMSCs inhibited TGF-β1-induced changes in human bronchial epithelial cell proliferation, apoptosis, secretion of TNF-α and IL-6 and activation of the Hedgehog pathway. In conclusion, UCMSCs have protective effects on TGF-β1-induced inflammation in human bronchial epithelial cells by regulating the Hedgehog pathway.

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Ethics Approval

This study was approved by the Ethics Committee of Fuzhou No.1 Hospital Affiliated with Fujian Medical University

How to Cite

Lin, Q., Yu, T., Li, X., Lin, X., Fan, Y., & Xu, L. (2023). Umbilical cord mesenchymal stem cells inhibited inflammation of bronchial epithelial cells by regulating Hedgehog pathway. European Journal of Histochemistry, 67(4). https://doi.org/10.4081/ejh.2023.3908
Copyright (c) 2023 The Author(s) Creative Commons License

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