https://doi.org/10.4081/ejh.2024.3950
Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles
Submitted: 22 December 2023
Accepted: 22 January 2024
Published: 29 January 2024
Accepted: 22 January 2024
Abstract Views: 808
PDF: 311
HTML: 2
HTML: 2
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This paper reviews some of the goals of our investigations published over the years on Rivista di Istochimica Normale e Patologica, Basic and Applied Histochemistry, and the European Journal of Histochemistry - EJH. In a series of papers, we published some of the basic cytochemical features of the sperm cytodifferentiation process for the first time. This was a conceptual and practical prerequisite to the in situ quantitative evaluation of sperm DNA content. We showed that the discrepancy between the expected 1:2 ratio when comparing sperm versus somatic cell DNA content (sperm DNA content is always far low from the theoretical value) is due to DNA losses caused by the hydrochloric treatment entailed by the Feulgen reaction. The knowledge of the specific losses that occur during the various steps of the Feulgen reaction has allowed us to use it critically in Genome Size studies to highlight: - sperm aneuploidy in chromosomally derived subfertility; - the broad variability range of Mammalian genome sizes; - that termites are roaches (after decades of discussion on this topic). In addition, in a seminal paper on human oocytes, we showed (by transmission electron microscopy) a specific chromatin and cytoplasmic organization (both essential for further embryo development) linked to oocyte maturation arrest, a datum quite relevant to treating unmet therapeutic needs in human and veterinary reproduction.
Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, Sutton GG, et al. The sequence of the human genome. Science 2001;291:1304-51.
DOI: https://doi.org/10.1126/science.1058040
Bernardi G. The compositional evolution of vertebrate genomes. Gene 2000;259:31-43.
DOI: https://doi.org/10.1016/S0378-1119(00)00441-8
Capanna E, Manfredi Romanini MG. Nuclear DNA content and morphology of the karyotype in certain Palearctic Microchiroptera. Caryologia 1971;24:471-82.
DOI: https://doi.org/10.1080/00087114.1971.10796455
Ohno S. Dispensable genes. Trends Genet 1985;1:160-4.
DOI: https://doi.org/10.1016/0168-9525(85)90070-8
Mazzini G. The Feulgen reaction: from pink-magenta to rainbow fluorescent. Eur J Histochem 2024 (in press).
Redi CA. [Limiti di quantitatività della reazione di Feulgen: analisi della interferenza esercitata dalla deistonizzazione e dai trattamenti di denaturazione e rinaturazione].[Article in Italian]. Riv Ist Norm Patol 1976;20:177-81.
Redi CA, Garagna S, Bottiroli G. Cytochemical evaluation of sperm and lymphocyte DNA content after treatment with 5 N HCl. Histochemistry 1986;84:41-4.
DOI: https://doi.org/10.1007/BF00493418
Davidson E, Hough B, Amenson S, Britten R. General interspersion of repetitive with non repetitive sequences elements in the DNA of Xenopus. J Mol Biol 1973;77:1-23.
DOI: https://doi.org/10.1016/0022-2836(73)90359-8
Redi CA, Manfredi Romanini MG. [Su alcune caratteristiche citochimiche della cromatina negli eritrociti di Xenopus laevis Daud].[Article in Italian]. Riv Ist Norm Patol 1976;20:183-96.
Redi CA, Garagna S, Pellicciari C. Cytochemical assessment of chromatin characteristics during sperm cytodifferentiation in the mouse. Bas Appl Histochem 1982;26:279-88.
Redi CA, Garagna S, Capanna E. Nature's experiments with in situ hybridation? A hypothesis for the mechanism of Rb fusion. J Evol Biol 1990;3:133-7.
DOI: https://doi.org/10.1046/j.1420-9101.1990.3010133.x
Capanna E, Redi CA. Chromosomes and microevolutionary processes. Boll Zool 1994;61:285-94.
DOI: https://doi.org/10.1080/11250009409355897
Redi CA, Garagna S, Zuccotti M, Capanna E. Genome size: a novel genome signature in support of Afrotheria. J Mol Evol 2007;64:484-6.
DOI: https://doi.org/10.1007/s00239-006-0237-1
Helgen KM. Evolution. The mammal family tree. Science 2011;334:458-9.
DOI: https://doi.org/10.1126/science.1214544
Redi CA, Capanna E. Genome size evolution: sizing mammalian genome. Cytogenet Genome Res 2012;137:97-112.
DOI: https://doi.org/10.1159/000338820
McKittrick FA. A contribution to the understanding of cockroach-termite affinities. Ann Entomol Soc Am 1965;58:18-22.
DOI: https://doi.org/10.1093/aesa/58.1.18
Djernaes M, Klass KD, Eggleton P. Identifying possible sister groups of Cryptocercidae + Isoptera: a combined molecular and morphological phylogeny of Dictyoptera. Mol Phylogenet Evol 2015;84:284-303.
DOI: https://doi.org/10.1016/j.ympev.2014.08.019
Monti M, Redi CA, Capanna E. Genome size evaluations in cockroaches: new entries. Eur J Histochem 2022;66:3400.
DOI: https://doi.org/10.4081/ejh.2022.3400
Monti M, Calligaro A, Behr B, Rejo Pera R, Redi CA, Wossidlo M. Functional topography of the fully grown human oocyte. Eur J Histochem 2017;61:32-5.
DOI: https://doi.org/10.4081/ejh.2017.2769
Veiga M, Camia B, Diepenbrock S, Redi CA, Casasco A, Monti M. Artificial intelligence selection of viable oocytes from phase contrast images for in vitro maturation and fertilization. Biol Reprod 2023;109:386-9.
DOI: https://doi.org/10.1093/biolre/ioad084
How to Cite
Capanna, E., & Redi, C. (2024). Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles. European Journal of Histochemistry, 68(1). https://doi.org/10.4081/ejh.2024.3950
Copyright (c) 2024 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Yu Wang, Ziyi Wang, Wenyang Yu, Xia Sheng, Haolin Zhang, Yingying Han, Zhengrong Yuan, Qiang Weng, Seasonal expressions of androgen receptor, estrogen receptors and cytochrome P450 aromatase in the uteri of the wild Daurian ground squirrels (Spermophilus dauricus) , European Journal of Histochemistry: Vol. 62 No. 1 (2018)
- I. Golic, K. Velickovic, M. Markelic, A. Stancic, A. Jankovic, M. Vucetic, V. Otasevic, B. Buzadzic, B. Korac, A. Korac, Calcium-induced alteration of mitochondrial morphology and mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes , European Journal of Histochemistry: Vol. 58 No. 3 (2014)
- G. Cutroneo, G. Vermiglio, A. Centofanti, G. Rizzo, M. Runci, A. Favaloro, M.G. Piancino, P. Bracco, G. Ramieri, F. Bianchi, F. Speciale, A. Arco, F. Trimarchi, Morphofunctional compensation of masseter muscles in unilateral posterior crossbite patients , European Journal of Histochemistry: Vol. 60 No. 2 (2016)
- C. Dall'Oca, T. Maluta, F. Cavani, G.P. Morbioli, P. Bernardi, A. Sbarbati, D. Degl'Innocenti, B. Magnan, The biocompatibility of porous vs non-porous bone cements: a new methodological approach , European Journal of Histochemistry: Vol. 58 No. 2 (2014)
- M.I. Dávila-RodrÃguez, Elva I. Cortés-Gutiérrez, R.M. Cerda Flores, M. Pita, J.L. Fernández, C. López-Fernández, J. Gosálvez, Constitutive heterochromatin polymorphisms in human chromosomes identified by whole comparative genomic hybridization , European Journal of Histochemistry: Vol. 55 No. 3 (2011)
- Proshanta Roy, Ilenia Martinelli, Michele Moruzzi, Federica Maggi, Consuelo Amantini, Maria Vittoria Micioni Di Bonaventura, Carlo Cifani, Francesco Amenta, Seyed Khosrow Tayebati, Daniele Tomassoni, Ion channels alterations in the forebrain of high-fat diet fed rats , European Journal of Histochemistry: Vol. 65 No. s1 (2021): Special Collection on Advances in Neuromorphology in Health and Disease
- H. Zhang, X. Guo, S. Zhong, T. Ge, S. Peng, P. Yu, Z. Zhou, Heterogeneous vesicles in mucous epithelial cells of posterior esophagus of Chinese giant salamander (Andrias davidianus) , European Journal of Histochemistry: Vol. 59 No. 3 (2015)
- V. Lodde, S. C. Modina, F. Franciosi, E. Zuccari, I. Tessaro, A. M. Luciano, Localization of DNA methyltransferase-1 during oocyte differentiation, in vitro maturation and early embryonic development in cow , European Journal of Histochemistry: Vol. 53 No. 4 (2009)
- A. Busato, P. Fumene Feruglio, P.P. Parnigotto, P. Marzola, A. Sbarbati, In vivo imaging techniques: a new era for histochemical analysis , European Journal of Histochemistry: Vol. 60 No. 4 (2016)
- F. Perdoni, M. Falleni, D. Tosi, D, Cirasola, S. Romagnoli, P. Braidotti, E. Clementi, G. Bulfamante, E. Borghi, A histological procedure to study fungal infection in the wax moth Galleria mellonella , European Journal of Histochemistry: Vol. 58 No. 3 (2014)
<< < 8 9 10 11 12 13 14 15 16 17 > >>
You may also start an advanced similarity search for this article.
