https://doi.org/10.4081/ejh.2024.4021
The role of miRNA-29b1 on the hypoxia-induced apoptosis in mammalian cardiomyocytes
Submitted: 12 March 2024
Accepted: 6 May 2024
Published: 27 June 2024
Accepted: 6 May 2024
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Cardiomyocyte apoptosis is a complex biological process involving the interaction of many factors and signaling pathways. In hypoxic environment, cardiomyocytes may trigger apoptosis due to insufficient energy supply, increased production of oxygen free radicals, and disturbance of intracellular calcium ion balance. The present research aimed to investigate the role of microRNA-29b1 (miR-29b1) in hypoxia-treated cardiomyocytes and its potential mechanism involved. We established an in vitro ischemia model using AC16 and H9C2 cardiomyocytes through hypoxia treatment (1% O2, 48 h). Cell apoptosis was evaluated by flow cytometry using Annexin V FITC-PI staining assay. Moreover, we used Western blot and immunofluorescence analysis to determine the expression of Bcl-2, Bax caspase-3 and Cx43 proteins. We found that miR-29b1 protected AC16 and H9C2 cells from hypoxia-induced injury as evidence that miR-29b1 attenuated the effects of hypoxia treatment on AC16 and H9C2 cell apoptosis after hypoxia treatment. In conclusion, our findings suggest that miR-29b1 may have potential cardiovascular protective effects during ischemia-related myocardial injury.
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Supporting Agencies
National Natural Science Foundation of ChinaHow to Cite
Dai, B., Liu, H., Juan, D., Wu, K., & Cao, R. (2024). The role of miRNA-29b1 on the hypoxia-induced apoptosis in mammalian cardiomyocytes. European Journal of Histochemistry, 68(3). https://doi.org/10.4081/ejh.2024.4021
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