https://doi.org/10.4081/ejh.2024.4052
CCL18 promotes endometriosis by increasing endometrial cell migration and neuroangiogenesis
Submitted: 22 April 2024
Accepted: 10 July 2024
Published: 6 August 2024
Accepted: 10 July 2024
Abstract Views: 315
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Endometriosis is an estrogen-dependent inflammatory gynecological disease whose pathogenesis is unclear. C-C motif chemokine ligand 18 (CCL18), a chemokine, is involved in several inflammatory diseases. In this study, we aimed to investigate the role of CCL18 in endometriosis and its underlying mechanisms. Human endometrium and peritoneal fluid were obtained from women with and without endometriosis for molecular studies. The expression level of CCL18 in each tissue sample was examined by RNA sequencing analysis, quantitative PCR analysis and immunohistochemistry staining. The effects of CCL18 on cell migration, tube formation and neurite growth were investigated in vitro using primary endometrial cells, human umbilical vein endothelial cells (HUVECs) and dorsal root ganglion (DRG) neurons, respectively. Moreover, the development of endometriosis in mice was studied in vivo by blocking CCL18. CCL18 was shown to be overexpressed in endometrial foci and peritoneal fluid in women with endometriosis and was positively correlated with endometriosis pain. In vitro, CCL18 promoted the migration of ectopic endometrial cells, tube formation of HUVECs, and nerve outgrowth of DRG neurons. More importantly, inhibition of CCL18 significantly suppressed lesion development, angiogenesis, and nerve infiltration in a mouse model of endometriosis. In conclusion, CCL18 may play a role in the progression of endometriosis by increasing endometrial cell migration and promoting neuroangiogenesis.
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Ethics Approval
the experimental protocols were approved by the Ethics Committee of the Women’s Hospital, Zhejiang University School of Medicine., The treatment of the mice in this study was approved by the Ethics Committee of Animal Laboratory, Zhejiang UniversitySupporting Agencies
Natural Science Foundation of Zhejiang Province, National Natural Science Foundation of ChinaHow to Cite
Peng, Y., Ding, S., Xu, P., Zhang, X., Wang, J., Li, T., … Zhang, X. (2024). CCL18 promotes endometriosis by increasing endometrial cell migration and neuroangiogenesis. European Journal of Histochemistry, 68(3). https://doi.org/10.4081/ejh.2024.4052
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