https://doi.org/10.4081/ejh.2024.4106
Low ozone concentrations do not exert cytoprotective effects on tamoxifen-treated breast cancer cells in vitro
Submitted: 11 July 2024
Accepted: 2 September 2024
Published: 9 September 2024
Accepted: 2 September 2024
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Medical treatment with low ozone concentrations proved to exert therapeutic effects in various diseases by inducing a cytoprotective antioxidant response through the nuclear factor erythroid derived-like 2 (Nrf2) transcription factor pathway. Low ozone doses are increasingly administered to oncological patients as a complementary treatment to mitigate some adverse side-effects of antitumor treatments. However, a widespread concern exists about the possibility that the cytoprotective effect of Nrf2 activation may confer drug resistance to cancer cells or at least reduce the efficacy of antitumor agents. In this study, the effect of low ozone concentrations on tamoxifen-treated MCF7 human breast cancer cells has been investigated in vitro by histochemical and molecular techniques. Results demonstrated that cell viability, proliferation and migration were generally similar in tamoxifen-treated cells as in cells concomitantly treated with tamoxifen and ozone. Notably, low ozone concentrations were unable to overstimulate the antioxidant response through the Nfr2 pathway, thus excluding a possible ozone-driven cytoprotective effect that would lead to increased tumor cell survival during the antineoplastic treatment. These findings, though obtained in an in vitro model, support the hypothesis that low ozone concentrations do not interfere with the tamoxifen-induced effects on breast cancer cells.
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How to Cite
Inguscio, C. R., Carton, F., Cisterna, B., Rizzi, M., Boccafoschi, F., Tabaracci, G., & Malatesta, M. (2024). Low ozone concentrations do not exert cytoprotective effects on tamoxifen-treated breast cancer cells <i>in vitro</i>. European Journal of Histochemistry, 68(3). https://doi.org/10.4081/ejh.2024.4106
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