https://doi.org/10.4081/ejh.2024.4140

Effect of Danggui Buxue decoction on hypoxia-induced injury of retinal Müller cells in vitro

Vol. 68 No. 4 (2024)
Submitted: 10 September 2024
Accepted: 29 October 2024
Published: 25 November 2024
Danggui Buxue decoction, hypoxia, retinal Müller cells, diabetic retinopathy, ATF4/CHOP pathway
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Authors

Xilin Ge
Diabetes Research Institute, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen; Geriatric Department, Zhongshan Hospital (Xiamen), Fudan University, Xiamen, China.
Caoxin Huang
Diabetes Research Institute, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China.
Wenting Chen
Diabetes Research Institute, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Chen Yang
Diabetes Research Institute, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Wenfang Huang
Diabetes Research Institute, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Jia Li
Diabetes Research Institute, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Shuyu Yang
gexilin@aliyun.com
Traditional Chinese Medicine Studio, The First Affiliated Hospital of Xiamen University, Xiamen; Integrated Traditional Chinese and Western Medicine Center, School of Medicine, Xiamen University, Xiamen, China.

Retinopathy is a common complication of diabetes mellitus and the leading cause of visual impairment. Danggui Buxue decoction (RRP) has been used as a traditional drug for the treatment of diabetic nephropathy for many years. The aim of this study was to investigate the effects of RRP on hypoxia-induced retinal Müller cell injury. A model of retinal Müller cell damage was created using high glucose levels (25 mmol/L) and/or exposure to low oxygen conditions (1% O2). RRP was given to rats by continuous gavage for 7 days to obtain drug-containing serum. After sterilization, the serum was added to the culture medium at a ratio of 10%. Cell viability, apoptosis, and cell proliferation were assessed using the CCK-8 kit, Annexin V-FITC/propidium iodide apoptosis kit, and EdU kit. The mRNA levels of angiogenesis factors (ANGPTL4, VEGF) and inflammatory factors (IL-1B, ICAM-1) were detected by RT-qPCR. Western blot analysis was employed to assess the levels of proteins related to the ATF4/CHOP pathway. Following hypoxia for 48 h and 72 h, there was a significant decrease in cell viability and proliferation, as well as a notable increase in apoptosis compared to the control group (21% O2). However, high glucose stimulation had no significant effect, and high glucose combined with hypoxia had no further damage to cells. After 48 h of exposure to low oxygen levels, the mRNA expression levels of ANGPTL4, VEGF, IL-1B, and ICAM-1 in retinal Müller cells were significantly higher than in the control group (21% O2). RRP treatment significantly alleviated the increase of cell apoptosis and the upregulation of IL-1B and-1 in retinal Müller cells induced by hypoxia. RRP has the potential to reduce the suppression of the ATF4/CHOP pathway in hypoxia-induced retinal Müller cells, and it significantly alleviates cell apoptosis through regulating inflammatory factors and the ATF4/CHOP pathway.

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Ethics Approval

this study was approved by the Ethics Committee of Xiamen University

How to Cite

Ge, X., Huang, C., Chen, W., Yang, C., Huang, W., Li, J., & Yang, S. (2024). Effect of Danggui Buxue decoction on hypoxia-induced injury of retinal Müller cells <i>in vitro</i>. European Journal of Histochemistry, 68(4). https://doi.org/10.4081/ejh.2024.4140
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