Restorative effects of camellia oil on the skin-barrier function in a model of DNCB-induced atopic dermatitis

Shicheng Jiao; Lijun Deng; Mu Niu; Jie Yang

doi:10.4081/ejh.2025.4147

Authors

Shicheng Jiao

Jiao Shicheng Medical Beauty Clinic, Haikou, Hainan, China.

Lijun Deng

Department of Dermatology, Wuzhong People’s Hospital, Suzhou, Jiangsu, China.

Mu Niu

Department of Dermatology, The Fifth People’s Hospital of Hainan Province, Affiliated Dermatology Hospital of Hainan Medical University, Haikou, Hainan, China.

Jie Yang

13337564169@163.com

Department of Dermatology, The Fifth People’s Hospital of Hainan Province, Affiliated Dermatology Hospital of Hainan Medical University, Haikou, Hainan, China.

This study aimed to evaluate the therapeutic efficacy of camellia oil on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice, as well as its effect on the expression of skin-barrier-related proteins. A mouse model of AD was created via topical application of DNCB; subsequently, the animals were randomly divided into four groups: the blank control (Control), model (Model), moisturizing cream (Moisturizer), and camellia oil (Camellia) groups. The Camellia group received camellia oil, whereas the Moisturizer group was treated with moisturizing cream, as a positive control. Skin lesions, ear and back tissue morphology, and the serum levels of IgE, IL-4, and IFN-γ were analyzed. Compared with the Control group, AD mice exhibited erythema, papules, dryness, peeling, and significantly higher serum IgE and IL-4 levels. Compared with the Model group, treatment with camellia oil and moisturizing cream considerably reduced skin inflammation, ear thickness, and scratching frequency. A histopathological analysis revealed that camellia oil reduced inflammatory-cell infiltration and edema in the AD-affected skin. Furthermore, camellia oil upregulated filaggrin (FLG), thus aiding in skin-barrier repair. These findings suggest that camellia oil significantly improves AD symptoms, enhances FLG expression, and restores the damaged skin barrier in AD mouse models.

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Ethics Approval

the experiment was authorized by the Animal Ethics Committee of the Fifth people's Hospital of Hainan Province

Supporting Agencies

Hainan Province Clinical Medical Center , Hainan Provincial Natural Science Foundation of China

How to Cite

Jiao, S., Deng, L., Niu, M., & Yang, J. (2025). Restorative effects of camellia oil on the skin-barrier function in a model of DNCB-induced atopic dermatitis. European Journal of Histochemistry, 69(1). https://doi.org/10.4081/ejh.2025.4147

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