https://doi.org/10.4081/ejh.2025.4159
Immunohistochemistry of carbonic anhydrases I, II and VI in the rat lingual serous salivary glands of von Ebner
Submitted: 7 November 2024
Accepted: 17 February 2025
Published: 4 March 2025
Accepted: 17 February 2025
Abstract Views: 460
PDF: 29
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Carbonic anhydrase (CA) has been localized to many structures involved in ion transport including the acini and ducts of the major (parotid, sublingual and submandibular) salivary glands of humans and rodents. It also has been localized by enzyme histochemistry and by immunohistochemistry for CA isoenzyme VI (CA VI) to the acini and ducts of rat serous lingual glands of von Ebner. The purpose of this study was to explore the intracellular distribution by cell type of three CA isoenzymes in these glands. Immunohistochemistry was undertaken with antibodies to human CAs I, II and VI in paraffin sections of rat tongues that had been fixed in Helly’s fluid. The density of the reaction product was scored as 0 (none) to 5 (strongest). Reactions in the acini with CA I and II antibodies were weak luminally to moderate basally and generally moderate, respectively, moderate in the intercalated ducts, and moderate basally to strong luminally in the excretory ducts. Weak to moderate CA VI reactions occurred in the acini and ducts. The stronger luminal reactions to CAs I and II in the excretory ducts suggest that they contribute to pH regulation in the saliva of von Ebner’s glands via HCO3- transport.
Downloads
Download data is not yet available.
Publication Facts
Metric
This article
Other articles
Peer reviewers
2
2.4
Reviewer profiles N/A
Author statements
Author statements
This article
Other articles
Data availability
N/A
16%
External funding
N/A
32%
Competing interests
N/A
11%
Metric
This journal
Other journals
Articles accepted
57%
33%
Days to publication
116
145
- Editor & editorial board
- profiles
- Academic society
- N/A
- Publisher
- PAGEPress Publications, Pavia, Italy
To learn about these publication facts, click 
PF is maintained by the Public Knowledge Project
Citations
1. Carpentier P, Chetail M, Fournie J. [Mise en evidence de deux anhydrases carbonique au niveau du complexe salivare sous-mandibulare-sublingual principal chez le rat].[Article in Fench]. J Biol Buccale 1980;8:33-47.
2. Ogawa Y, Fernley T, Ito R, Ijuhin N. Immnunohistochemistry of carbonic anhydrase isozymes VI and II during development of the rat salivary glands. Histochem Cell Biol 1998;110: 81-8.
DOI: https://doi.org/10.1007/s004180050268
3. Parkkila S, Kaunisto K, Rajaniemi L, Kumpulainan T, Jokinen K, Rajaniemi H. Immunohistochemical localization of carbonic anhydrase isoenzymes VI, II and I in human parotid and submandibular glands. J Histochem Cytochem 1990;38:941-7.
DOI: https://doi.org/10.1177/38.7.2113069
4. Piras M, Tandler B, Barbarossa IT, Piludu M. Immunogold labeling of carbonic anhydrase isozyme (CA-VI) in secretory granules of human parotid glands. Acta Histochem 2012;114: 406-8.
DOI: https://doi.org/10.1016/j.acthis.2011.08.004
5. Ohana E. Transepithelial transport across duct cells of the salivary gland. Oral Dis 2015;21:826-35.
DOI: https://doi.org/10.1111/odi.12201
6. Hennigar RA, Schulte BA, Spicer SS. Immunolocalization of carbonic anhydrase isozymes in rat and mouse salivary and exorbital lacrimal glands. Anat Rec 1983;207:605-14.
DOI: https://doi.org/10.1002/ar.1092070408
7. Noda Y, Sumitomo S, Orito T, Mori M. Histochemical localization of carbonic anhydrase I and II in the submandibular glands of mouse, rat, hamster and guinea pig. Arch Oral Biol 1986;31:795-800.
DOI: https://doi.org/10.1016/0003-9969(86)90130-5
8. Ogawa Y, Chang C-K, Kuwahara H, Hong SS, Toyosawa S, Yagi T. Immunoelectron microscopy of carbonic anhydrase isozyme VI in rat submandibular gland: comparison with isozymes I and II. J Histochem Cytochem 1992;40:807-17.
DOI: https://doi.org/10.1177/40.6.1588027
9. Noda Y, Sumitomo S, Hikosaka N, Mori M. Immunohistochemical observations on carbonic anhydrase I and II in human salivary glands and obstructive sialadenitis. J Oral Pathol 1986;15:187-90.
DOI: https://doi.org/10.1111/j.1600-0714.1986.tb00604.x
10. Ogawa Y, Hong SS, Toyosawa S, Kuwahara H, Shimakazi M, Yagi T. Immunoelectron microscopy of carbonic anhydrase isozyme VI in human submandibular gland: comparison with isozymes I and II. J Histochem Cytochem 1993;41:343-51.
DOI: https://doi.org/10.1177/41.3.8429198
11. Brown D, Garcia-Segura LM, Orci L. Carbonic anhydrase is associated with taste buds in rat tongue. Brain Res 1984;324:346-8.
DOI: https://doi.org/10.1016/0006-8993(84)90046-5
12. Leinonen, Parkkila S, Kaunisto P, Rajniemi H. Secretion of carbonic anhydrase VI from human and rat lingual von Ebner’s glands. J Histochem Cytochem 2001;49:657-82.
DOI: https://doi.org/10.1177/002215540104900513
13. Tandler B. Ultrastructure of the duct system in mammalian salivary glands. Micros Res Tech 1993;28:57-74.
DOI: https://doi.org/10.1002/jemt.1070260107
14. Carpentier P, Chetail M, Fournie J. Carbonic anhydrase in the rat salivary glands: distribution and ultrastructural localization. Biol Cell 1985;54:241-50.
DOI: https://doi.org/10.1111/j.1768-322X.1985.tb00400.x
15. Hand AR. The fine structure of von Ebner’s gland of the rat. J Cell Biol 1970;44:340-53.
DOI: https://doi.org/10.1083/jcb.44.2.340
16. Peagler FD, Redman RS, McNutt RL, Kruse DH, Johansson I. Enzyme histochemical and immunohistochemical localization of carbonic anhydrase as a marker of ductal differentiation in the developing rat parotid gland. Anat Rec 1998;250:190-8.
DOI: https://doi.org/10.1002/(SICI)1097-0185(199802)250:2<190::AID-AR9>3.0.CO;2-7
17. Redman RS, Peagler FD, Johansson I. Immunohistochemical localization of carbonic anhydrases I, II and VI in the developing rat sublingual and submandibular glands. Anat Rec 2000;258:69-76.
DOI: https://doi.org/10.1002/(SICI)1097-0185(20000301)258:3<269::AID-AR6>3.3.CO;2-P
18. Hamosh M, Hand AR. Development of secretory activity in serous cells of the rat tongue. Cytological differentiation and accumulation of lingual lipase. Dev Biol 1978;65:100-13.
DOI: https://doi.org/10.1016/0012-1606(78)90183-5
19. Kock K, Bläker M, Schmale M. Postnatal development of von Ebner’s glands: accumulation of a protein of the lipocalin family in taste papillae of rat tongue. Cell Tissue Res 1992;267:313-20.
DOI: https://doi.org/10.1007/BF00302970
20. Murakami H, Sly WS. Purification and characterization of human salivary carbonic anhydrase. J Biol Chem 1987;262:1382-8.
DOI: https://doi.org/10.1016/S0021-9258(19)75797-4
21. Luna LG. Manual of histologic staining methods of the Armed Forces Institute of Pathology, 3rd ed., New York: McGraw-Hill; 1968.
22. Hewett-Emmett D, Tashian RE. Functional diversity, conservation, and convergence of the α, β and γ carbonic anhydrase gene families. Mol Phylogenet Evol 1996;5:50-77.
DOI: https://doi.org/10.1006/mpev.1996.0006
23. Carter N, Jeffery S, Shiels A. Immunoassay of carbonic anhydrase III in rat tissues. FEBS Lett 1982;139:265-66.
DOI: https://doi.org/10.1016/0014-5793(82)80866-1
24. Riley DA, Ellis S, Bain J. Carbonic anhydrase activity in skeletal muscle fiber types, spindles, and capillaries of rat soleus and extensor digitorum longus muscles. J Histochem Cytochem 1982;30:1275-88.
DOI: https://doi.org/10.1177/30.12.6218195
25. Jeffery S, Carter ND, Smith A. Immunocytochemical localization of carbonic anhydrase isozymes I, II and III in rat skeletal muscle. J Histochem Cytochem 1986;34: 13-6.
DOI: https://doi.org/10.1177/34.4.2936798
26. Bernfeld B. Amylases, α & β. In: Colowick SP, Kaplan NO (eds.), Methods in Enzymology, vol. 1. New York: Academic Press; 1955. pp. 149-58.
DOI: https://doi.org/10.1016/0076-6879(55)01021-5
27. Field RB, Hand AR. Secretion of lingual lipase and amylase from rat lingual serous glands. Am J Physiol 1987; 253: G217-25.
DOI: https://doi.org/10.1152/ajpgi.1987.253.2.G217
28. Henkin R, Martin BM, Agarwal RP. Efficacy of exogenous oral zinc in treatment of patients with carbonic anhydrase VI deficiency. Am J Med Sci 1999;318:392-405.
DOI: https://doi.org/10.1016/S0002-9629(15)40664-0
29. Ueno K, Hirono C, Kitagaw M, Shiba Y, Sugita M. Different rate-limiting activities of intracellular pH regulators for HCO3- secretion stimulated by forskolin and carbachol in rat parotid intralobular ducts. J Physiol Sci 2016;66:477-90.
DOI: https://doi.org/10.1007/s12576-016-0443-6
30. Azzali G, Bucci G, Gatti R, Orlandini G, Ferrari G. Fine structure of the excretory system of the deep posterior (Ebner’s) salivary glands of the human tongue. Acta Anat (Basel) 1989;136:257-68.
DOI: https://doi.org/10.1159/000146835
31. Testa Riva F, Cossu M, Lantini MS, Riva A. Fine structure of human deep posterior lingual glands. J Anat 1985;142:103-15.
32. Goto T, Shirakawa H, Furukawa Y, Komai M. Decreased expression of carbonic anhydrase isozyme II, rather than of isozyme VI, in submandibular glands in long-term zinc-deficient rats. Brit J Nutr 2007;99:248-53.
DOI: https://doi.org/10.1017/S0007114507801565
Supporting Agencies
United States Department of Veterans Affairs, Institute for Clinical Research, Inc., Washington, DCHow to Cite
Redman, R. S. (2025). Immunohistochemistry of carbonic anhydrases I, II and VI in the rat lingual serous salivary glands of von Ebner. European Journal of Histochemistry, 69(1). https://doi.org/10.4081/ejh.2025.4159
Copyright (c) 2025 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.
Similar Articles
- Manxiu Cao, Lei Zhang, Jiaqi Chen, Cangyu Wang, Junhong Zhao, Xiang Liu, Yongjing Yan, Yue Tang, Zixiu Chen, Haihong Li, Differential antigen expression between human apocrine sweat glands and eccrine sweat glands , European Journal of Histochemistry: Vol. 67 No. 1 (2023)
- D. Kluchova, A. Bolekova, C. Heichel, A. J. Bron, I. Kozak, NADPH-diaphorase expression in the Meibomian glands of rat palpebra in postnatal development , European Journal of Histochemistry: Vol. 54 No. 4 (2010)
- T. Karaca, Y. Hulya Uz, R. Karabacak, I. Karaboga, S. Demirtas, A. Cagatay Cicek, Effects of hyperthyroidism on expression of vascular endothelial growth factor (VEGF) and apoptosis in fetal adrenal glands , European Journal of Histochemistry: Vol. 59 No. 4 (2015)
- S. Gurzu, M. Krause, I. Ember, L. Azamfirei, G. Gobel, K. Feher, I. Jung, Mena, a new available marker in tumors of salivary glands? , European Journal of Histochemistry: Vol. 56 No. 1 (2012)
- W.J. Liu, J. Yang, Developmental expression of inositol 1, 4, 5-trisphosphate receptor in the post-natal rat cochlea , European Journal of Histochemistry: Vol. 59 No. 2 (2015)
- Tadashi Yasui, Hiroshi Gomi, Taishi Kitahara, Azuma Tsukise, Ultrastructure and immunohistochemical characterization of proteins concerned with the secretory machinery in goat ceruminous glands , European Journal of Histochemistry: Vol. 61 No. 3 (2017)
- A. Bolekova, D. Kluchova, L. Tomasova, N. Hvizdosova, Effect of retinoic acid on the nitrergic innervation of meibomian glands in rats , European Journal of Histochemistry: Vol. 56 No. 4 (2012)
- E. Tarantola, V. Bertone, G. Milanesi, E. Capelli, A. Ferrigno, D. Neri, M. Vairetti, S. Barni, I. Freitas, Dipeptidylpeptidase-ÂIV, a key enzyme for the degradation of incretins and neuropeptides: activity and expression in the liver of lean and obese rats , European Journal of Histochemistry: Vol. 56 No. 4 (2012)
- E. Lacunza, V. Ferretti, C. Barbeito, A. Segal-Eiras, M. V. Croce, Immunohistochemical evidence of Muc1 expression during rat embryonic development , European Journal of Histochemistry: Vol. 54 No. 4 (2010)
- Tadashi Yasui, Kenya Miyata, Chie Nakatsuka, Azuma Tsukise, Hiroshi Gomi, Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland , European Journal of Histochemistry: Vol. 65 No. 4 (2021)
You may also start an advanced similarity search for this article.
