Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR
AbstractSpecific viral laboratory diagnosis of primary Epstein-Barr Virus (EBV) infection is usually based on antibody-detection assays. However, molecular detection is also considered the reference standard assay for diagnosis of central nervous system infections and of most cases of nasopharyngeal carcinoma (NPC). One-step or nested polymerase chain reaction (PCR) has rapidly replaced immunological assays based on virus-specific Ig antibodies for the laboratory diagnosis of Herpesvirus infections, even if serological methods are considered an additional tool for defining clinical diagnosis. In this article, we will present a rapid, sensitive and robust molecular tool for the viral detection of EBV (EBNA-1) within tissue specimens by making use of in situ PCR (IS-PCR).
- Abstract views: 330
- PDF: 559
Copyright (c) 2009 N Marziliano, E Arbustini, M Rossi de Gasperis, S Crovella
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.