Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR

Abstract

Specific viral laboratory diagnosis of primary Epstein-Barr Virus (EBV) infection is usually based on antibody-detection assays. However, molecular detection is also considered the reference standard assay for diagnosis of central nervous system infections and of most cases of nasopharyngeal carcinoma (NPC). One-step or nested polymerase chain reaction (PCR) has rapidly replaced immunological assays based on virus-specific Ig antibodies for the laboratory diagnosis of Herpesvirus infections, even if serological methods are considered an additional tool for defining clinical diagnosis. In this article, we will present a rapid, sensitive and robust molecular tool for the viral detection of EBV (EBNA-1) within tissue specimens by making use of in situ PCR (IS-PCR).

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Published
2009-06-30
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How to Cite
Marziliano, N., Arbustini, E., Rossi de Gasperis, M., & Crovella, S. (2009). Detection of Epstein Barr virus in formalin-fixed paraffin tissues by fluorescent direct in situ PCR. European Journal of Histochemistry, 49(3), 309-312. https://doi.org/10.4081/958