Immunohistochemical localization of IGF-I, IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax)

  • G. Radaelli | giuseppe.radaelli@unipd.it University of Padua, Italy.
  • C. Poltronieri
  • C. Simontacchi
  • E. Negrato
  • F. Pascoli
  • A. Libertini
  • D. Bertotto

Abstract

The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triploids. From day 30 of larval life, IGF-I immunoreactivity observed in skeletal muscle, intestine, gills and kidney was stronger in triploids than in diploids. At day 30, triploids exhibited a standard length significantly higher than the one of diploids. Although IGF-II and MSTN immunoreactivity was detectable in different tissues and organs, no differences between diploids and triploids were observed. The spatial localization of IGF-I, IGF-II and MSTN proteins detected in this study is in agreement with previous findings on the distribution of these proteins in diploid larvae and fry. The highest IGF-I immunoreactivity observed in triploids suggests a possible involvement of ploidy in their growth performance.

Downloads

Download data is not yet available.

Author Biography

G. Radaelli, University of Padua
Department of Experimental Veterinary Sciences, University of Padua
Published
2010-04-14
Section
Original Papers
Supporting Agencies
Italian Ministero dell’Università e della Ricerca Scientifica e Tecnologica (MIUR) and University of Padua (Progetto di Ateneo).
Keywords:
IGF-I, IGF-II, MSTN, immunohistochemistry, triploid
Statistics
Abstract views: 568

PDF: 206
HTML: 4878
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Radaelli, G., Poltronieri, C., Simontacchi, C., Negrato, E., Pascoli, F., Libertini, A., & Bertotto, D. (2010). Immunohistochemical localization of IGF-I, IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax). European Journal of Histochemistry, 54(2), e16. https://doi.org/10.4081/ejh.2010.e16

Most read articles by the same author(s)