Original Papers
14 April 2010
Vol. 54 No. 2 (2010)
https://doi.org/10.4081/ejh.2010.e16

Immunohistochemical localization of IGF-I, IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax)

Authors

G. Radaelli
giuseppe.radaelli@unipd.it
University of Padua, Italy.
C. Poltronieri
C. Simontacchi
E. Negrato
F. Pascoli
A. Libertini
D. Bertotto
The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triploids. From day 30 of larval life, IGF-I immunoreactivity observed in skeletal muscle, intestine, gills and kidney was stronger in triploids than in diploids. At day 30, triploids exhibited a standard length significantly higher than the one of diploids. Although IGF-II and MSTN immunoreactivity was detectable in different tissues and organs, no differences between diploids and triploids were observed. The spatial localization of IGF-I, IGF-II and MSTN proteins detected in this study is in agreement with previous findings on the distribution of these proteins in diploid larvae and fry. The highest IGF-I immunoreactivity observed in triploids suggests a possible involvement of ploidy in their growth performance.

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Supporting Agencies

Italian Ministero dell’Università e della Ricerca Scientifica e Tecnologica (MIUR) and University of Padua (Progetto di Ateneo).
G. Radaelli, University of Padua
Department of Experimental Veterinary Sciences, University of Padua

How to Cite



Immunohistochemical localization of IGF-I, IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax). (2010). European Journal of Histochemistry, 54(2), e16. https://doi.org/10.4081/ejh.2010.e16

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