https://doi.org/10.4081/ejh.2010.e16

Immunohistochemical localization of IGF-I, IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax)

Vol. 54 No. 2 (2010)
Submitted: 23 December 2009
Accepted: 12 February 2010
Published: 14 April 2010
IGF-I, IGF-II, MSTN, immunohistochemistry, triploid
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Authors

G. Radaelli
giuseppe.radaelli@unipd.it
University of Padua, Italy.
C. Poltronieri
C. Simontacchi
E. Negrato
F. Pascoli
A. Libertini
D. Bertotto
The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triploids. From day 30 of larval life, IGF-I immunoreactivity observed in skeletal muscle, intestine, gills and kidney was stronger in triploids than in diploids. At day 30, triploids exhibited a standard length significantly higher than the one of diploids. Although IGF-II and MSTN immunoreactivity was detectable in different tissues and organs, no differences between diploids and triploids were observed. The spatial localization of IGF-I, IGF-II and MSTN proteins detected in this study is in agreement with previous findings on the distribution of these proteins in diploid larvae and fry. The highest IGF-I immunoreactivity observed in triploids suggests a possible involvement of ploidy in their growth performance.

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Supporting Agencies

Italian Ministero dell’Università e della Ricerca Scientifica e Tecnologica (MIUR) and University of Padua (Progetto di Ateneo).
G. Radaelli, University of Padua
Department of Experimental Veterinary Sciences, University of Padua

How to Cite

Radaelli, G., Poltronieri, C., Simontacchi, C., Negrato, E., Pascoli, F., Libertini, A., & Bertotto, D. (2010). Immunohistochemical localization of IGF-I, IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax). European Journal of Histochemistry, 54(2), e16. https://doi.org/10.4081/ejh.2010.e16

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