Koilocytes are enriched for alkaline-labile sites

  • E. I. Cortés-Gutiérrez | elvacortes@cibinmty.net Instituto Mexicano del Seguro Social, Mexico.
  • M. I. Dávila-Rodríguez Instituto Mexicano del Seguro Social, Mexico.
  • J. L. Fernández Complejo Hospitalario Universitario Juan Canalejo, Spain.
  • C. López-Fernández Universidad Autónoma de Madrid, Spain.
  • J. Gosálvez Universidad Autónoma de Madrid, Spain.

Abstract

This study investigated possible variations in the chromatin structure of koilocytes resulting from human papillomavirus (HPV) infection. Alkaline-labile sites (ALS) were detected with the DNA breakage detection–fluorescence in situ hybridization (DBD-FISH) technique using a whole human genome DNA probe obtained from individuals without koilocytosis. The variable levels of ALS present were measured quantitatively using image analysis after whole-genome DNA hybridization. A significant increase in the number of ALS was observed in koilocytes compared with normal cells. We demonstrated that the presence of ALS could be an indicator of chromatin change in koilocytes caused by HPV infection.

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Author Biographies

E. I. Cortés-Gutiérrez, Instituto Mexicano del Seguro Social
División de Genética. Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social
M. I. Dávila-Rodríguez, Instituto Mexicano del Seguro Social
División de Genética. Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social
J. L. Fernández, Complejo Hospitalario Universitario Juan Canalejo
Sección de Genética y Unidad de Investigación, Hospital Teresa Herrera, Complejo Hospitalario, Universitario Juan Canalejo
C. López-Fernández, Universidad Autónoma de Madrid
Universidad Autónoma de Madrid
J. Gosálvez, Universidad Autónoma de Madrid
Universidad Autónoma de Madrid
Published
2010-10-13
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Section
Original Papers
Keywords:
koilocytes, binucleated and multinucleated, alkaline-labile sites (ALS), DNA breakage damage–fluorescence in situ hybridization (DBD–FISH).
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How to Cite
Cortés-GutiérrezE. I., Dávila-RodríguezM. I., Fernández, J. L., López-FernándezC., & Gosálvez, J. (2010). Koilocytes are enriched for alkaline-labile sites. European Journal of Histochemistry, 54(4), e32. https://doi.org/10.4081/ejh.2010.e32

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