Distribution of NADPH-diaphorase and AChE activity in the anterior leaflet of rat mitral valve

  • K. Lovasova | kvetuse.lovasova@upjs.sk Department of Anatomy, Faculty of Medicine, P.J. Safarik University,, Slovakia.
  • D. Kluchova Department of Anatomy, Faculty of Medicine, P.J. Safarik University,, Slovakia.
  • A. Bolekova Department of Anatomy, Faculty of Medicine, P.J. Safarik University,, Slovakia.
  • F. Dorko Department of Anatomy, Faculty of Medicine, P.J. Safarik University,, Slovakia.
  • T. Spakovska Department of Anatomy, Faculty of Medicine, P.J. Safarik University,, Slovakia.

Abstract

The mitral valve, as an active flap, forms the major part of the left ventricular inflow tract and therefore plays an important function in many aspects of left ventricular performance. The anterior leaflet of this valve is the largest and most ventrally placed of two leaflets that come together during ventricular systole to close the left atrioventricular orifice. Various neurotransmitters are responsible for different functions including controlling valve movement, inhibiting or causing the failure of impulse conduction in the valve and the sensation of pain. Nitric oxide acts as a gaseous free radical neurotransmitter, neuromediator and effective cardiovascular modulator. Acetyl-choline is known to function as a typical neurotransmitter. Histochemical methods for detection of nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), as an indirect nitric oxide-synthase marker, and method for detection of acetylcholinesterase (AChE) were used. Both methods were performed on the same valve sample. A widespread distribution of nerve fibres was observed in the anterior leaflet of the mitral valve. The fine NADPH-d positive (nitrergic) nerve fibres were identified in all zones of valve leaflet. AChE positive (cholinergic) nerve fibres were identified forming dense network and fibres organized in stripes. Endocardial cells and vessels manifested heavy NADPH-d activity. Our observations suggest a different arrangement of nitrergic and cholinergic nerve fibres in the anterior leaflet of the mitral valve. The presence of nitrergic and cholinergic activity confirms the involvement of both neurotransmitters in nerve plexuses and other structures of mitral valve.

Downloads

Download data is not yet available.
Published
2010-02-04
Section
Original Papers
Keywords:
NADPH-diaphorase, acetylcholinesterase, heart, mitral valve, nerve fibres, vessels, rat
Statistics
Abstract views: 528

PDF: 103
HTML: 3029
Share it

PlumX Metrics

PlumX Metrics provide insights into the ways people interact with individual pieces of research output (articles, conference proceedings, book chapters, and many more) in the online environment. Examples include, when research is mentioned in the news or is tweeted about. Collectively known as PlumX Metrics, these metrics are divided into five categories to help make sense of the huge amounts of data involved and to enable analysis by comparing like with like.

How to Cite
Lovasova, K., Kluchova, D., Bolekova, A., Dorko, F., & Spakovska, T. (2010). Distribution of NADPH-diaphorase and AChE activity in the anterior leaflet of rat mitral valve. European Journal of Histochemistry, 54(1), e5. https://doi.org/10.4081/ejh.2010.e5